The monitoring of microvascular liver blood flow changes during ischemia and reperfusion using laser speckle contrast imaging

Li, Chong Hui; Wang, Hong Dong; Ji, Hu; Ge, Xin; Pan, Ke; Zhang, Ai Qun; Dong, Jia Hong

doi:10.1016/j.mvr.2014.04.010

Cited by 21 publications

(16 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…to the occlusion of the hepatic portal. 46 Hepatic ischemia-reperfusion injury reduces total hepatic blood flow and causes severe microcirculatory dysfunction, which results in venous stagnation, adversely affecting hepatic tissue oxygenation. 42 Since the hemoglobin absorbs light strongly in the wavelength range of 500 to 600 nm, the decrease in μ a at 500, 530, 570, and 585 nm [Figs.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of light scattering and absorption properties ofin vivorat liver using a single-reflectance fiber probe during preischemia, ischemia–reperfusion, and postmortem

et al. 2015

View full text Add to dashboard Cite

Diffuse reflectance spectroscopy (DRS) has been extensively used for characterization of biological tissues as a noninvasive optical technique to evaluate the optical properties of tissue. We investigated a method for evaluating the reduced scattering coefficient μ(s)', the absorption coefficient μ(a), the tissue oxygen saturation StO₂, and the reduction of heme aa3 in cytochrome c oxidase CcO of in vivo liver tissue using a single-reflectance fiber probe with two source-collector geometries. We performed in vivo recordings of diffuse reflectance spectra for exposed rat liver during the ischemia-reperfusion induced by the hepatic portal (hepatic artery, portal vein, and bile duct) occlusion. The time courses of μ a at 500, 530, 570, and 584 nm indicated the hemodynamic change in liver tissue as well as StO₂. Significant increase in μ(a)(605)/μ(a)(620) during ischemia and after euthanasia induced by nitrogen breathing was observed, which indicates the reduction of heme aa3, representing a sign of mitochondrial energy failure. The time courses of μ(s)' at 500, 530, 570, and 584 nm were well correlated with those of μ(a), which also reflect the scattering by red blood cells. On the other hand, at 700 and 800 nm, a temporary increase in μ(s)' and an irreversible decrease in μ(s)' were observed during ischemia-reperfusion and after euthanasia induced by nitrogen breathing, respectively. The change in μ(s)' in the near-infrared wavelength region during ischemia is indicative of the morphological changes in the cellular and subcellular structures induced by the ischemia, whereas that after euthanasia implies the hepatocyte vacuolation. The results of the present study indicate the potential application of the current DRS system for evaluating the pathophysiological conditions of in vivo liver tissue.

show abstract

Section: Discussionmentioning

confidence: 99%

Evaluation of light scattering and absorption properties ofin vivorat liver using a single-reflectance fiber probe during preischemia, ischemia–reperfusion, and postmortem

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Following PVF and HAF measurements, LBF of the whole liver was measured as previously described (18). Following ligation of the left and median liver lobes and clamping of the pedicle for the OPT group, or the portal vein for the OPV group, of the right liver lobe, the LBF of the right liver lobe was measured 5 min post-clamping.…”

Section: Methodsmentioning

confidence: 99%

Preserving hepatic artery flow during portal triad blood occlusion improves regeneration of the remnant liver in rats with obstructive jaundice following partial hepatectomy

Kong

et al. 2018

Exp Ther Med

Self Cite

View full text Add to dashboard Cite

In certain cases, major hepatectomy is essential and inevitable in patients with hilar cholangiocarcinoma and obstructive jaundice (OJ). The current study was designed to evaluate effects of a novel method of portal blood occlusion, where the portal vein was occluded (OPV) and the hepatic artery flow was preserved in rats with OJ that underwent partial hepatectomy. OJ was induced in rats by ligation of the common bile duct for 7 days. Subsequently, OJ rats underwent hepatectomy removing 76% of the liver following occlusion of the portal triad (OPT), OPV or without portal blood occlusion. Liver blood flow (LBF), liver damage and regeneration were assessed. The safety limit for the duration of liver ischemia was 20 min for OPT and 40 min for OPV in rats with OJ. OPT and OPV methods resulted in significantly decreased microvascular LBF in rats with OJ from 529.53±91.55 laser speckle perfusion units (LSPU) in the control to 136.89±32.32 and 183.99±49.25 LSPU, respectively. Liver damage was assessed analyzing levels of serum alanine transaminase and direct bilirubin, determining interleukin-1β and tumor necrosis factor-α expression and histological examination. It was demonstrated that liver damage and caspase-3 and −9 expression in the liver were substantially reduced in the OPV group compared with the OPT group. In addition, the OPV method significantly improved liver regeneration in OJ rats, as indicated by increased rates of liver regeneration and expression of proliferating cell nuclear antigen and Ki-67 compared with the OPT group. Therefore, the OPV method may prolong the duration of portal blood occlusion, reduce liver injury and improve liver regeneration by preserving hepatic arterial flow during portal blood control in rats with OJ undergoing partial hepatectomy. The current study describes a novel technique, which may be applied in liver surgery in patients with complex jaundice.

show abstract

“…15,16,18e21 This method is based on the laser Doppler method, but has a much higher spatial and temporal resolution and allows for imaging of large surface areas contact free in real time. 18 LSCI uses full field laser measurements for estimation of tissue perfusion and detects mean microcirculatory blood flow (mFlux) up to a tissue depth of 3 mm. It is contact free, non-invasive and has good reproducibility.…”

Section: Assessment Of Microcirculationmentioning

confidence: 99%

“…It is contact free, non-invasive and has good reproducibility. 22 To minimise measurement disturbances from organ movement, 18 the respiratory rate was held stable and a measurement period of several breathing cycles was used to reduce measurement variability. 23 The speckle laser was positioned 25 cm above the intestinal segment using a target laser.…”

Section: Assessment Of Microcirculationmentioning

confidence: 99%

Volume Based Resuscitation and Intestinal Microcirculation after Ischaemia/Reperfusion Injury: Results of an Exploratory Aortic Clamping Study in Pigs

Behem

Graessler

Pinnschmidt

et al. 2019

European Journal of Vascular and Endovascular Surgery

View full text Add to dashboard Cite

WHAT THIS PAPER ADDS The study provides further insight into the behaviour of intestinal microcirculatory blood flow after ischaemia/ reperfusion injury. The results of the study emphasise that volume therapy should be titrated with meticulous care, particularly after ischaemia/reperfusion injury, and that unnecessary fluid load may impair microcirculatory flow and worsen tissue oedema. Effects on tissue oxygen supply, tissue viability, and patient outcome should be addressed in further studies.Objectives: In the presence of ischaemia/reperfusion (I/R) induced endothelial injury, volume administration may not correlate with increased microcirculation. The aim of this study was to evaluate intestinal microcirculation after standardised sequential volume loading in an animal model of I/R injury following supracoeliac aortic clamping.Methods: This was a prospective exploratory pilot animal study. Intestinal I/R injury was induced in eight pigs during experimental thoraco-abdominal aortic repair. After 6 h of I/R, microcirculatory blood flow (mFlux, measured in the ileum using direct laser speckle contrast imaging) and macrohaemodynamic parameters (using trans-cardiopulmonary thermodilution) were measured and measurements were repeated after each of four sequential volume loading steps (VLS1 e 4). Each load was administered over 5 min followed by another 5 min for equilibration. Results: All animals survived until after VLS4. After 6 h of I/R cardiac output (CO) (p < .001) and mFlux (p < .001) had both decreased. CO increased again after VLS1 (p < .001) and VLS2 (p ¼ .036), whereas mFlux did not change. In contrast, mFlux further decreased after VLS3 (p < .01) and VLS4 (p < .001), whereas CO did not change anymore. Extravascular lung water continued to increase after VLS2 (p ¼ .046) and VLS4 (p ¼ .049). Conclusions: I/R leads to impaired intestinal microcirculation, which was not restored by volume administration in spite of improved CO. In contrast, further volume administration exceeding preload reserves was associated with additional decreases in the intestinal microcirculation. The potentially negative effect of excessive volume resuscitation after I/R injury should encourage further translational research.

show abstract

The monitoring of microvascular liver blood flow changes during ischemia and reperfusion using laser speckle contrast imaging

Cited by 21 publications

References 26 publications

Evaluation of light scattering and absorption properties ofin vivorat liver using a single-reflectance fiber probe during preischemia, ischemia–reperfusion, and postmortem

Evaluation of light scattering and absorption properties ofin vivorat liver using a single-reflectance fiber probe during preischemia, ischemia–reperfusion, and postmortem

Preserving hepatic artery flow during portal triad blood occlusion improves regeneration of the remnant liver in rats with obstructive jaundice following partial hepatectomy

Volume Based Resuscitation and Intestinal Microcirculation after Ischaemia/Reperfusion Injury: Results of an Exploratory Aortic Clamping Study in Pigs

Contact Info

Product

Resources

About