The Monitoring of Mycoplasma gallisepticum Minimum Inhibitory Concentrations during the Last Decade (2010–2020) Seems to Reveal a Comeback of Susceptibility to Macrolides, Tiamulin, and Lincomycin

Bottinelli, Marco; Gastaldelli, Michele; Picchi, Micaela; Dall'Ora, Arianna; Borges, Lorena Cristovao; Ramírez, Ana S.; Matucci, Andrea; Catania, Salvatore

doi:10.3390/antibiotics11081021

Cited by 6 publications

(2 citation statements)

References 77 publications

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“…The same study found four isolates with a higher MIC value of 1 mg × L −1 . The MIC value of 2 mg × L −1 was registered in another study [44]. These data confirm that doxycycline can be effective against M. gallisepticum and also indicate that it is advisable to use the tetracycline antibiotic in practice after the antimicrobial susceptibility test.…”

Section: Discussionsupporting

confidence: 61%

Population Pharmacokinetics of Doxycycline, Administered Alone or with N-Acetylcysteine, in Chickens with Experimental Mycoplasma gallisepticum Infection

2022

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Mycoplasmosis is a bacterial infection that significantly affects poultry production, and it is often controlled with antibiotics, including doxycycline. The conducted study aimed to determine population pharmacokinetic (PopPk) parameters of doxycycline in healthy (n = 12) and in Mycoplasma gallisepticum-challenged (n = 20) chickens after its oral administration via drinking water at the registered dose rate of 20 mg/kg b.w./24 h for five days, without or with co-administration of N-acetylcysteine (NAC, a dose of 100 mg/kg b.w./24 h) via the feed. Doxycycline concentrations in plasma were analyzed with the LC-MS/MS method. The values of tvV/F and tvke were 4.73 L × kg−1 and 0.154 h−1, respectively, and they showed low BSV. A high BSV of 93.17% was calculated for the value of tlag of 0.8 h, which reflects the inter-individual differences in the water consumption. PTA was computed after Monte Carlo simulation with the registered dose for doxycycline. The target of %fT > MIC ≥ 80% and 100% can be achieved in 90% of the broiler population, after a correction for protein binding, for bacteria with MIC ≤ 0.5 mg × L−1 and 0.25 mg × L−1, respectively. The applied PopPk model did not reveal significant effect of M. gallisepticum infection and co-administration of NAC on pharmacokinetic parameters of doxycycline.

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Section: Discussionsupporting

confidence: 61%

Population Pharmacokinetics of Doxycycline, Administered Alone or with N-Acetylcysteine, in Chickens with Experimental Mycoplasma gallisepticum Infection

2022

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“…The level of lincomycin in the liver has medicinal value in all groups, indicating the long-term persistence of the drug in the liver. Table-3 [ 12 , 13 , 17 , 18 , 34 , 35 ] compares the drug levels in broiler tissues with the MIC values of lincomycin from recent studies. Several antimicrobial drugs, such as tetracycline [ 25 ], enrofloxacin [ 36 ], florfenicol [ 37 ], and tilmicosin [ 38 ], have been reported to have higher doses in the liver than in the muscle or kidney of broilers.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic effects of lincomycin and level of drug degradation in broiler tissues after treatment

Wijayanti,

Muzaki,

Wibisono

et al. 2024

Vet World

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Background and Aim: Lincomycin is an antibiotic used in broiler farming and is commonly combined with other substances to achieve synergistic and complementary effects on the antibacterial spectrum and mechanism. We developed a specific high-performance liquid chromatography (HPLC) method to measure lincomycin levels in broiler tissues. This study aimed to determine the lincomycin level in tissues and compare it with the minimum inhibitory concentration (MIC) and maximum residue limit (MRL) of certain pathogenic bacteria. Materials and Methods: Three groups of broiler chickens were involved in the study (n = 20 in each group): A control group without lincomycin treatment and two groups (each further divided into two sub-groups) that received oral lincomycin at a dose of 1 g/10 kg of body weight daily for 7 and 14 consecutive days. Tissue samples were collected from each group 1 day and 1 week after lincomycin administration (ALA). This study validated the development of a technique for analyzing drug level degradation in tissues using HPLC. Descriptive and statistical analyses were performed for drug levels to assess their therapeutic value and safety based on lincomycin MIC of certain pathogenic bacteria and MRL. Results: The method validation resulted in linear regression and coefficient of determination for tissues with r2 > 0.99, with a recovery rate of 90%–110%, precision as the coefficient of variation 15%, and specificity with no peak overlap for lincomycin. The limits of detection for the liver and kidney were 0.01 μg/g, 0.05 μg/g, and 0.1 μg/g for the breast muscle and all tissues. Administration of lincomycin for 7 and 14 days resulted in therapeutic value concentrations. Lincomycin levels in the liver and kidney of ALA exceeded the MRL, whereas breast muscles were below the MRL for a week of ALA treatment. Conclusion: Administration of lincomycin for 7 and 14 consecutive days resulted in therapeutic value; however, after a week, most tissues showed high drug concentrations that exceeded the MRL. It is necessary to carefully consider the prolonged therapeutic dose of lincomycin in broilers. Antibiotic therapy must be guided in such a way as to protect the product from harmful residues. Keywords: : broiler tissues, lincomycin level, maximum residue limit, minimum inhibitory concentration.

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Potential biological active bacteriocin production by Bifidobacterium via eco‐friendly, low‐cost solid state fermentation principle

Priyanka,

Namasivayam,

Sudha

et al. 2024

Environmental Quality Mgmt

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The present study is undertaken to evaluate the potential biological activities of bacteriocin produced by the Lactic acid bacteria Bifidobacterium sp. adopting solid state fermentation. Bifidobacterium was isolated from cooked fermented rice water. Isolated bacterial strain was identified by morphological, biochemical, and molecular characteristics. Sterilized cooked rice was inoculated with bacterial inoculum followed by extraction of bacteriocin under aseptic condition. Extracted bacteriocin was screened for antibacterial, antioxidant, and anti‐inflammatory activities under invitro condition. Cooked fermentation rice medium used for solid state fermentation (SSF) supported growth and bacteriocin production by Bifidobacterium longum. Potential antibacterial activity of the extracted bacteriocin was confirmed by growth inhibition of Bacillus subtilis, and Escherichia coli strain. Notable free radical scavenging activity at 120 μg/mL concentration of bacteriocin clearly indicated antioxidant activity. Anti‐inflammatory activity of the bacteriocin was determined which showed maximum inhibition at 120 μg/mL concentration. All these finding suggest the possible utilization of bacteriocin extraction from Bifidobacterium as an effective, safe bioactive agent.

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The Monitoring of Mycoplasma gallisepticum Minimum Inhibitory Concentrations during the Last Decade (2010–2020) Seems to Reveal a Comeback of Susceptibility to Macrolides, Tiamulin, and Lincomycin

Cited by 6 publications

References 77 publications

Population Pharmacokinetics of Doxycycline, Administered Alone or with N-Acetylcysteine, in Chickens with Experimental Mycoplasma gallisepticum Infection

Population Pharmacokinetics of Doxycycline, Administered Alone or with N-Acetylcysteine, in Chickens with Experimental Mycoplasma gallisepticum Infection

Therapeutic effects of lincomycin and level of drug degradation in broiler tissues after treatment

Potential biological active bacteriocin production by Bifidobacterium via eco‐friendly, low‐cost solid state fermentation principle

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