“…When both P and Q are chiral, the universal amalgamation is in fact rooted. For example, the universal amalgamation of {4, 4} (1,3) with {4, 4} (1,3) has 24 vertices, whereas the universal amalgamation of {4, 4} (3,1) with {4, 4} (1,3) has 50 vertices [9].…”