The mononuclear phagocyte system of the mouse defined by immunohistochemical localization of antigen F4/80. Relationship between macrophages, Langerhans cells, reticular cells, and dendritic cells in lymphoid and hematopoietic organs.

Abstract

The immune response genes of the major histocompatibility gene complex code for surface antigens (Ia) now believed to be involved in the interaction between T cells and accessory or "antigen-presenting" cells (1, 2). Whilst some groups suggest that Ia antigens present on subpopulations of mononuclear phagocytes are involved in T cell activation (1-4), others have presented evidence for the involvement of a separate Ia + cell population (5-8) now generally referred to as dendritic cells. The in vivo correlate of the isolated antigen-presenting dendritic cell is not yet clear. The availability of a specific monoclonal antibody against mouse dendritic cells (9) will be helpful in clarifying this question. One obvious candidate is the Ia + "interdigitating cell" that has been described in thymus and in T cell-dependent areas of lymphoid organs (10-15). However, whilst the isolated splenic dendritic cell has virtually none of the functional characteristics of a mononuclear phagocyte (6), it has been suggested that interdigitating cells are members of the mononuclear phagocyte system and are related to epidermal Langerhans cells and similar cells .found in afferent lymphatics (14).Apart from T cell activation during an immune response, macrophages have also been implicated in the control of hematopoiesis in the bone marrow (16)(17)(18)(19)(20), the differentiation of thymocytes in the thymus (21),. proliferation and keratinization of squamous epithelial cells (Langerhans cells [22][23][24]), and the antibody response of B lymphocytes to T-independent antigens (25).In order to determine the roles of mononuclear phagocytes and other cells in these systems, it is necessary to have reliable surface markers analogous to those that have delineated subclasses of T lymphocytes (26). F4/80 is a hybridoma that secretes a noncytotoxic rat IgG2b directed against a 160-kdalton plasma membrane antigen of mouse mononuclear phagocytes (27).

Oncogenes belonging to the CSF-1 transduction pathway direct p53 tumor suppressor effects to monocytic differentiation in 32D cells

“…Consistently with the morphological data, 32D-fms [S301,F969] cells express higher levels of the CD11b and F4-80 monocyte/macrophage cell surface antigens (Larson and Springer, 1990;Austiyn and Gordon, 1981;Hume et al, 1983) than 32D-src cells (1.7-fold for CD11b, and 3.6-fold for F4-80). Wt-p53 over- expression was able to increase the levels of these two antigens in 32D-src cells after four days of incubation at the permissive temperature (1.9-fold for CD11b, and 1.4-fold for F4-80), while no signi®cant increase was found in the 32Dfms-transformed cells, even after 10 days of incubation at the permissive temperature (1.2-fold for CD11b and 0.9-fold for F4-80), or 3 weeks post-infection (1.06-fold for CD11b and 1.08-fold for F4-80).…”

EMR1, the human homolog of F4/80, is an eosinophil‐specific receptor

“…No expression of EMR1 was detected on any of these cells (data not shown). Next, we tested splenic and peritoneal macrophages, which are known to abundantly express F4/80 + in mice [2,6]. However, in humans, these resident cells were EMR1 -, even after permeabilization (Fig.…”

“…Due to its cell specificity, the F4/80 antigen has been of crucial importance, initially, for the characterization of the murine mononuclear phagocyte system [2,3] and, later, for the detection of macrophages in innumerable in vivo studies. F4/80 is highly expressed on resident tissue phagocytic cells including macrophages in the BM, the thymic cortex, the lymph node medulla, and the splenic red pulp, Kupffer cells in the liver, Langerhans cells in the skin, and microglia in the central nervous system.…”

Intracellular replication ofSalmonella typhimuriumstrains in specific subsets of splenic macrophagesin vivo

“…Three monoclonal antibodies raised in the same host (F4/80, anti-scavenger receptor/ 2F8 and anti-sialoadhesin/CD169) were pooled to provide a pan-macrophage marker. The F4/80 antibody recognizes mature macrophages mainly in the red pulp of the spleen (Austyn and Gordon, 1981;Hume et al, 1983), whereas the 2F8 and anti-sialoadhesin antibodies recognize different subsets of spleen marginal zone macrophages (Crocker and Gordon, 1989;Crocker et al, 1991;Fraser et al, 1993;Hughes et al, 1995). In all experiments, appropriate isotype controls were included for gate setting.…”