The monovalent cation "leak" transport in human erythrocytes: an electroneutral exchange process

Richter, Steffi; Hamann, J.; Kummerow, D.; Bernhardt, Ingolf

doi:10.1016/s0006-3495(97)78106-2

Cited by 39 publications

(13 citation statements)

References 40 publications

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“…The first evidence for the involvement of two separate pathways in the enhancement of unidirectional Na Although it is now recognised that the first pathway is electrogenic [6,7] whereas the latter is electroneutral [8,9], a common pathway underlying these two transport modes cannot be entirely discounted [14]. However, as has been speculated elsewhere [15,16], it was assumed that the increase of the cation fluxes for RBCs suspended in LIS solutions is mediated by the K It is important to note that, after transferring cells from HIS to LIS solutions, a new steady-state is quickly established, associated with an increase of the internal pH from 7.2 to approximately 7.7 [9] which results in a depolarization from -10 mV to 18.5 mV.…”

Section: Discussionmentioning

confidence: 99%

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Differential Effect of HOE642 on Two Separate Monovalent Cation Transporters in the Human Red Cell Membrane

Bernhardt¹,

Weiss²,

Robinson³

et al. 2007

Cell Physiol Biochem

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Residual K⁺ fluxes in red blood cells can be stimulated in conditions of low ionic strength. Previous studies have identified both the non-selective, voltage-dependent cation (NSVDC) channel and the K⁺ (Na⁺)/H⁺ exchanger as candidate pathways mediating this effect, although it is possible that these pathways represent different modes of operation of a single system. In the present study the effects of HOE642, recently characterised as an inhibitor of the K⁺(Na⁺)/H⁺ exchanger, on NSVDC has been determined to clarify this question. Radioisotope flux measurements and conductance determinations showed that HOE642 exerted differential effects on the NSVDC channel and the K⁺(Na⁺)/H⁺ exchanger, confirming that the salt loss observed in low ionic strength solutions represents contributions from at least two independent ion transport pathways. The findings are discussed in the context of red blood cell apoptosis (eryptosis) and disease.

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Section: Discussionmentioning

confidence: 99%

“…At present, 10 different cation transport pathways and 5 Na + -dependent amino acid transporters have been characterised that can translocate Na + and/or K + across the membrane [4,5] [8,9]. In different studies, both pathways have been identified as the 602 principal pathway responsible for enhanced cation fluxes in LIS media.…”

Section: Introductionmentioning

confidence: 99%

Differential Effect of HOE642 on Two Separate Monovalent Cation Transporters in the Human Red Cell Membrane

Bernhardt¹,

Weiss²,

Robinson³

et al. 2007

Cell Physiol Biochem

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“…The increased K + efflux of irradiated erythrocytes correlates with the increase of the K + concentration of the supernatant during the storage [22]. This K + 'leak' is due to a transport molecule and no passive event as assumed so far [23]. Energy doses in the range of 15 to 30 Gy provide comparable fluxes [21].…”

Section: Discussionmentioning

confidence: 99%

Influence of 30 Gy Gamma Irradiation on the Quality of Red Blood Cell Concentrates in Several Storage Media

Bäumler¹,

Radtke²,

Haas³

et al. 1999

Transfus Med Hemother

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Background: The transfusion-associated graft versus host disease (t-GvHD) occurring in immunosuppressed patients can be prevented by irradiation of red blood cell concentrates (RCCs) with gamma irradiation at a dose of 30 Gy prior to transfusion. The aim of this study was to investigate, whether the irradiation of leukocyte-depleted RCCs in different storage media (SAG-M, PAGGS-M, or Adsol) may cause deterioration of red blood cells (RBC), resulting in a shortened shelf life of the RCC. Material and Methods:According to manufacturing (manually, apheresis), storage medium used (SAG-M, PAGGS-M, Adsol) and processing of leukocyte depletion (filtered, nonfiltered), 192 RCCs were separated into 8 groups with 24 RCCs each. Each group was divided into two subgroups. One was irradiated (30 Gy), the other one was used as control. Until the end of shelf life, pH, the concentrations of Na⁺ , K⁺ , lactate, glucose, 2,3-diphosphoglycerate (2,3-DPG) and adenosine triphosphate (ATP), parameters of blood cell count, and external hemoglobin were measured every week. Results:The metabolic parameters did not show significant differences between nonirradiated and irradiated RCCs. K⁺ efflux and hemolysis were significantly higher in the irradiated RCCs after the first week of storage. The values of hemolysis remained below the recommended limit of 0.8% of the hemoglobin content of RCCs until the 28th day of storage. After 28 days of storage the mean values of K⁺ increased by 25% compared to the control samples at the end of shelf life. The mean corpuscular volume (MCV) was significantly higher in the irradiated RCCs with significant differences between the various storage media. Leukocyte-depleted RCCs showed partly smaller rates of hemolysis than unfiltered RCCs. Conclusions:The determined in vitro parameters of irradiated and leukocyte-depleted RCCs stored in SAG-M, PAGGS-M or Adsol revealed no significant quantitative changes until 28th day of storage compared to those of nonirradiated RCCs.

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“…In our previous investigations we were able to demonstrate that the LIS effect can be easily ascribed to a K + (Na + )/H + exchanger. This explanation is based on findings that the residual K + transport does not depend on the transmembrane potential [8,9,10], theoretical calculations [11], and a demonstration of a 1 : 1 exchange of K + and H + under LIS conditions [12]. In addition, the non-selective, voltage-dependent cation (NSVDC) channel has to be taken into consideration [13,14,15].…”

Section: Introductionmentioning

confidence: 99%

Transmembrane Potential of Red Blood Cells Under Low Ionic Strength Conditions

Moersdorf

Egée

Hahn

et al. 2013

Cell Physiol Biochem

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Background/Aims: In a variety of investigations described in the literature it was not clear to what extent the transmembrane potential red blood cells (RBCs) was changed after the cells have been transferred into low ionic strength (LIS) solutions. Another open question was to find out how fast the transmembrane potential of RBCs in LIS solution will change and which final new equilibrium value will be reached. Methods: The transmembrane potential of human and bovine RBCs was investigated using the potential-sensitive fluorescent dye DIBAC₄(3) (bis(1,3-dibutylbarbituric acid) trimethine oxonol) as well as the CCCP (carbonylcyanide-m-chlorophenylhydrazone) method. Results: Under physiological conditions the transmembrane potential was about -10 mV in agreement with literature data. However, when the RBCs were transferred into an isosmotic low ionic strength medium containing sucrose the transmembrane potential increased to +73 mV and +81 mV for human and bovine RBCs, respectively. In case of human RBCs it continuously decreased reaching finally an equilibrium state of -10 mV again after 30 - 60 min. For bovine RBCs the transmembrane potential declined more slowly reaching a value of +72 mV after 30 min. Conclusions: Investigations of parameters of RBCs depending on transmembrane potential cannot be performed with human RBCs in LIS media.

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The monovalent cation "leak" transport in human erythrocytes: an electroneutral exchange process

Cited by 39 publications

References 40 publications

Differential Effect of HOE642 on Two Separate Monovalent Cation Transporters in the Human Red Cell Membrane

Differential Effect of HOE642 on Two Separate Monovalent Cation Transporters in the Human Red Cell Membrane

Influence of 30 Gy Gamma Irradiation on the Quality of Red Blood Cell Concentrates in Several Storage Media

Transmembrane Potential of Red Blood Cells Under Low Ionic Strength Conditions

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