The Montpellier<i>Leishmania</i>Collection, from a Laboratory Collection to a Biological Resource Center: A 39-Year-Long Story

Pratlong, Francine; Balard, Yves; Lami, Patrick; Talignani, Loïc; Ravel, Christophe; Dereure, J.; Lefebvre, Michèle; Serres, Ghislaine; Bastien, Patrick; Dedet, Jean-Pierre

doi:10.1089/bio.2015.0101

Cited by 2 publications

(1 citation statement)

References 74 publications

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“…It can take days to weeks to produce a result by culture methods, which are also expensive and labour-intensive to set up [ 86 ]. Furthermore, the distribution, transport and storage of cultures and culture material, including antibiotics used to prevent contamination from other microorganisms, make it an impractical method in many clinical settings [ 93 , 94 ].…”

Section: Methods For the Detection And Diagnosis Of Leishmaniasismentioning

confidence: 99%

Laboratory diagnostics for human Leishmania infections: a polymerase chain reaction-focussed review of detection and identification methods

et al. 2022

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Leishmania infections span a range of clinical syndromes and impact humans from many geographic foci, but primarily the world’s poorest regions. Transmitted by the bite of a female sand fly, Leishmania infections are increasing with human movement (due to international travel and war) as well as with shifts in vector habitat (due to climate change). Accurate diagnosis of the 20 or so species of Leishmania that infect humans can lead to the successful treatment of infections and, importantly, their prevention through modelling and intervention programs. A multitude of laboratory techniques for the detection of Leishmania have been developed over the past few decades, and although many have drawbacks, several of them show promise, particularly molecular methods like polymerase chain reaction. This review provides an overview of the methods available to diagnostic laboratories, from traditional techniques to the now-preferred molecular techniques, with an emphasis on polymerase chain reaction-based detection and typing methods. Graphical abstract

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Section: Methods For the Detection And Diagnosis Of Leishmaniasismentioning

confidence: 99%

Laboratory diagnostics for human Leishmania infections: a polymerase chain reaction-focussed review of detection and identification methods

et al. 2022

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Leishmaniasis in deployed military populations: A systematic review and meta-analysis

Niba Rawlings,

Bailey,

Courtenay

2024

Preprint

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Leishmaniasis affects military personnel deployed to endemic areas following exposure to sand flies infected with the protozoa Leishmania. This systematic review and meta-analysis of data specific to military populations aims to identify knowledge gaps to mitigate sand fly exposure and Leishmania transmission during deployments. The review was registered on PROSPERO (CRD42023463687). Random-effects meta-analyses and narrative synthesis were performed. Thirty-six studies were included, most of which reported on cutaneous leishmaniasis (CL), showing a mean incidence of 10% (95% CI: 5–16), suggesting higher rates in the Eastern Mediterranean region (14% [95% CI: 12–16]) compared to the African region (8%) and American region (9%). Asymptomatic Leishmania infection had a cumulative incidence of 11% (95% CI: 6–17), with higher rates in Eastern Mediterranean countries (20% [95% CI: 14–25]). Diagnosis involved parasitological, serological, and molecular methods, with L. mexicana and L. (V.) braziliensis identified as the predominant CL pathogens in deployed troops in the Americas. Visceral leishmaniasis cases were less frequent, all reported from the Eastern Mediterranean and associated with the Leishmania donovani/infantum complex; whereas CL cases in the Old World were due predominantly to L. major and L. tropica. Regular use of long-lasting insecticidal nets to mitigate sand fly exposure demonstrated high potential effectiveness than other reported personal protective measures (PPMs) which yielded mixed or inconclusive results. In summary, the systematic review revealed the substantial variability between study designs and statistical integrity. There is need for more consistent and robustly designed studies including well-define controls and replication. Future studies would be advised to explore the long-term effectiveness and practicality of PPMs, both individually and in combination, across diverse deployment settings.

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The MontpellierLeishmaniaCollection, from a Laboratory Collection to a Biological Resource Center: A 39-Year-Long Story

Cited by 2 publications

References 74 publications

Laboratory diagnostics for human Leishmania infections: a polymerase chain reaction-focussed review of detection and identification methods

Laboratory diagnostics for human Leishmania infections: a polymerase chain reaction-focussed review of detection and identification methods

Leishmaniasis in deployed military populations: A systematic review and meta-analysis

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