The more insect trypanosomatids under study-the more diverse Trypanosomatidae appears

Podlipaev, S. A.

doi:10.1016/s0020-7519(01)00139-4

Cited by 75 publications

(61 citation statements)

References 14 publications

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“…It was anticipated that an additional problem might be associated with presumed 'mixed' infections (the cases where more than one trypanosomatid species is present simultaneously within the same individual host), especially when the parasites have different cultivation properties (Podlipaev, 2000(Podlipaev, , 2001). An uncultivable or poorly cultivable organism might be predominant in a natural infection, and its morphology would be used to describe a novel species.…”

Section: Introductionmentioning

confidence: 99%

Selective recovery of the cultivation-prone components from mixed trypanosomatid infections: a case of several novel species isolated from Neotropical Heteroptera

Yurchenko

Lukeš

Jirků

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Mixed trypanosomatid infections (a simultaneous presence of two or more parasites in the same host) have long been suspected to represent an obstacle for recovering cultures that would faithfully represent original species descriptions. However, without the means to directly compare the parasites in the host and in culture, this would remain just a possibility. Here we have used PCR-based genotyping of spliced leader RNA gene repeats to analyse several novel species of insect trypanosomatids isolated from heteropteran hosts and to compare them with the parasites that had been detected in the gut smears of the same hosts. We have found that, whereas the original infections were dominated by some blastocrithidia-like parasites, most of the respective axenic cultures contained novel species of Crithidia and Leptomonas. Therefore, we concluded that, in each case, this replacement was caused by differences in cultivation properties between the original predominant blastocrithidia and the less fastidious parasite that was later recovered in culture. The properties of the new organisms, including their morphology and ultrastructure, as well as their phylogenetic affinities within the family, were investigated and used to describe five novel species.

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Section: Introductionmentioning

confidence: 99%

Selective recovery of the cultivation-prone components from mixed trypanosomatid infections: a case of several novel species isolated from Neotropical Heteroptera

Yurchenko

Lukeš

Jirků

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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“…However, for identification purposes, these criteria proved to be impractical and insufficient, because the same trypanosomatid species may be recovered from diverse species of insects and the same insect species may harbor various species of trypanosomatids. In addition, the morphology of trypanosomatid cells can be modified by environmental factors (Podlipaev, 2001;Momen, 2001Momen, , 2002. Therefore, there is a need to develop more effective means of trypanosomatid identification.…”

Section: Proteases Produced By Herpetomonas Species: Taxonomic Markermentioning

confidence: 99%

Applications of Zymography (Substrate-SDS-PAGE) for Peptidase Screening in a Post-Genomic Era

d’Avila-Levy¹,

Santos²,

Cuervo³

et al. 2012

Gel Electrophoresis - Advanced Techniques

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“…Poinar and Poinar have described two species of trypanosome parasites from the gut and proboscis of sandflies (Diptera: Psychodidae) embedded in amber from the Dominican Republic and Myanmar, and Trypanosoma flagellates in a fecal pellet adjacent to an assassin bug (Hemiptera: Reduviidae) in Dominican amber (11)(12)(13)(14). Although many trypanosomes are restricted to a single insect host, a few genera are heteroxenous and have a life cycle that requires both blood-sucking insect and vertebrate hosts (15). Given the similarities of the fossilized trypanosomes to known extant heteroxenous species, and the hematophagic lifestyle of the extant relatives of the insect hosts, Poinar has concluded that these fossils represent examples of hematophagy.…”

mentioning

confidence: 99%

Hemoglobin-derived porphyrins preserved in a Middle Eocene blood-engorged mosquito

Greenwalt¹,

Goreva

Siljeström

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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Significance Fossils, in addition to documenting the existence of extinct species, can often provide information on the behavior of ancient organisms. The present study describes the fossil of a blood-engorged mosquito in oil shale from northwestern Montana. The existence of this rare specimen extends the existence of blood-feeding behavior in this family of insects 46 million years into the past. Heme, the oxygen-carrying group of hemoglobin in the host’s blood, was identified in the abdomen of the fossil mosquito by nondestructive mass-spectrometry analysis. Although large and fragile molecules such as DNA cannot survive fossilization, other complex organic molecules, in this case iron-stabilized heme, can survive intact and provide information relative to the mechanisms of the fossilization process.

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The more insect trypanosomatids under study-the more diverse Trypanosomatidae appears

Cited by 75 publications

References 14 publications

Selective recovery of the cultivation-prone components from mixed trypanosomatid infections: a case of several novel species isolated from Neotropical Heteroptera

Selective recovery of the cultivation-prone components from mixed trypanosomatid infections: a case of several novel species isolated from Neotropical Heteroptera

Applications of Zymography (Substrate-SDS-PAGE) for Peptidase Screening in a Post-Genomic Era

Hemoglobin-derived porphyrins preserved in a Middle Eocene blood-engorged mosquito

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