The morphology of porcine oocytes is associated with zona pellucida glycoprotein transcript contents

Jackowska, M.; Kempisty, Bartosz; Antosik, Paweł; Bukowska, Dorota; Budna, Joanna; Lianeri, Margarita; Rosińska, Ewa; Woźna, M.; Jagodzińśki, Paweł P.; Jaśkowski, J. M.

doi:10.1016/s1642-431x(12)60097-7

Cited by 28 publications

(31 citation statements)

References 13 publications

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“…The usefulness of this method was documented by Szczepanska et al (2009), who assessed the spectral characteristics of porcine oocytes isolated from different sizes of follicles. They showed that oocytes collected from each class of follicular size after classic optical microscopy evaluation had similar morphology ("looked the same") in relation to morphological assessed factors described previously by Jackowska et al (2009). However, the "ideal" group of gametes recovered from the same size follicles differed significantly with special respect to their transmittance, spectrum and absorption of specific wavelengths.…”

Section: Microfluidic Chip (Lab-on-chip) Evaluation Of Oocytes and Emmentioning

confidence: 66%

The invasive and new non-invasive methods of mammalian oocyte and embryo quality assessment: a review

Bukowska¹,

Kempisty²,

Piotrowska³

et al. 2012

Vet. Med. - Czech

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ABSTRACT:The quality of oocytes-embryos can be determined by several techniques, including morphological, molecular, cellular and biochemical ones. The morphological methods of female gamete or embryo quality assessment often use thе following in vitro manipulation procedures such as: in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro embryo production (IVP). However, these methods are highly subjective and the morphological classification of oocytes or embryos is not always compatible with their ability to grow and develop. Additionally, molecular biology methods are objective and present parametric results, which are more or less comparable to the real oocyte-embryo "health". Although these techniques enable us to determine markers of oocyte-embryo developmental potential, when applied they lead to destruction of the analysed cells. Therefore, the need still exists to search for new methods that will be highly objective (parametric) and, which is most important, non-invasive. In this review, the morphological and molecular methods of oocyte-embryo quality assessment are presented. Moreover, we described a new system based on microfluidic technology (Lab-on-Chip) which allows the creation of a new device for mammalian oocyte as well as embryo quality evaluation: by using their spectral characterisation following embryo transfer (ET) procedures in the cattle and the pig.

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Section: Microfluidic Chip (Lab-on-chip) Evaluation Of Oocytes and Emmentioning

confidence: 66%

The invasive and new non-invasive methods of mammalian oocyte and embryo quality assessment: a review

Bukowska¹,

Kempisty²,

Piotrowska³

et al. 2012

Vet. Med. - Czech

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“…Conversely, it was demonstrated that high quality oocytes used in IVF were not influenced by semen pre-incubation. This occurs because the oocytes of better quality are more efficient in blocking polyspermy, in comparison to their low quality counterparts (JACKOWSKA et al, 2009). This reinforces that pre incubation does not affect the viability of sperm cells, but only facilitates low quality oocytes recognition for sperm penetration.…”

Section: Discussionmentioning

confidence: 99%

Pre-incubation of porcine semen reduces the incidence of polyspermy on embryos derived from low quality oocytes

et al. 2016

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“…Thereafter, the ovaries were placed in 5% fetal bovine serum solution (FBS) (Sigma-Aldrich Co., St. Louis, MO, USA) in phosphate buffered saline (PBS), and COCs were recovered in a sterile Petri dish by puncturing individual follicles 3-5 mm in size. COCs were washed three times in modified PBS, supplemented with 36 µg/ml pyruvate, 50 µg/ml gentamicin and 0.5 mg/ml bovine serum albumin (BSA) (Sigma-Aldrich, St. Louis, MO, USA) and then morphologically evaluated under a stereoscopic microscope, using the four-grade scale suggested by Jackowska et al (2009). Only grade-I COCs (n =100), which have a homogeneous cytoplasm and a complete and compact cumulus oophorus, were used in further steps of the experiment.…”

Section: Collection Of Porcine Ovaries and Classification Of Cumulus-mentioning

confidence: 99%

“…Total RNA was isolated from oocytes before (n = 40) and after IVM (n = 40), using an RNeasy mini column (Qiagen GmbH, Hilden, Germany) (Kempisty et al, 2008;Kempisty et al, 2009;Jackowska et al, 2009). The RNA samples were resuspended in RNase-free water and stored in liquid nitrogen.…”

Section: Real-time Quantitative Pcr (Rq-pcr) Analysis Of Cdk4 and Cx4mentioning

confidence: 99%

Expression and cellular distribution of cyclin-dependent kinase 4 (Cdk4) and connexin 43 (Cx43) in porcine oocytes before and after in vitro maturation

Kempisty

Ziółkowska

Piotrowska

et al. 2014

Acta Veterinaria Hungarica

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It is recognised that connexin 43 (Cx43) and cyclin-dependent kinase 4 (Cdk4) are involved in the cumulus cell-oocyte communication via gap junctions and the control of cell cycle progress. However, little is known about their mRNA expression pattern and encoded proteins distribution in porcine oocytes during in vitro maturation (IVM). Cumulus-oocyte complexes (COCs) were collected from 31 puberal crossbred Landrace gilts and analysed for their Cdk4 and Cx43 mRNA expression using RQ-PCR and for the respective protein expression by confocal microscopic observations. An increased Cdk4 and Cx43 mRNA expression was found in oocytes after IVM (P < 0.001 and P < 0.05, respectively). Confocal microscopic observations revealed a significant increase of Cdk4 protein expression in the cytoplasm of oocytes during the maturation process. The localisation of Cx43 changed from zona pellucida before to cytoplasm of oocytes after IVM. It is supposed that the increased expression of Cdk4 and Cx43 mRNA in oocytes after IVM is linked with the accumulation of a large amount of templates during the process of oocyte maturation. The translocation especially of Cx43 from the zona pellucida into the cytoplasm may be associated with a decrease in gap junction activity in fully grown porcine oocytes. Both Cdk4 and Cx43 can be used as 'checkpoints' of oocyte maturation.

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The morphology of porcine oocytes is associated with zona pellucida glycoprotein transcript contents

Cited by 28 publications

References 13 publications

The invasive and new non-invasive methods of mammalian oocyte and embryo quality assessment: a review

The invasive and new non-invasive methods of mammalian oocyte and embryo quality assessment: a review

Pre-incubation of porcine semen reduces the incidence of polyspermy on embryos derived from low quality oocytes

Expression and cellular distribution of cyclin-dependent kinase 4 (Cdk4) and connexin 43 (Cx43) in porcine oocytes before and after in vitro maturation

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