The morphology of the polyhedra of a host range-expanded recombinant baculovirus and its parents

Woo, Soo Dong; Kim, W. J.; Kim, H. S.; Jin, Byung Rae; Lee, Y. H.; Kang, Soo‐Kyung

doi:10.1007/s007050050368

Cited by 19 publications

(14 citation statements)

References 9 publications

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“…We have reported that the morphology of polyhedra is controlled not only by the polyhedrin, but also by the host and/or viral factors other than polyhedrin (Woo et al, 1998). We also showed that substitution of the polyhedrin gene between NPVs results in a change in polyhedra morphology.…”

Section: Introductionmentioning

confidence: 74%

“…We also examine the polyhedra morphology of wild-type AcNPV and another recombinant virus, AcBmPol, which produces BmNPV polyhedrin instead of AcNPV polyhedrin and has been described previously (Woo et al, 1998). We suggest that the shape and uniformity of polyhedra depend on some viral gene other than the polyhedrin gene, and that the size of the polyhedra is influenced by a host cell factor.…”

Section: Introductionmentioning

confidence: 85%

“…The cells infected with virus were floated by gentle pipetting and harvested at 1, 2, 3, 4 and 5 d-p.i. Polyhedra were prepared based on the previous method (Woo et al, 1998). Cells and polyhedra were pelleted by centrifugation and then the cell pellets were resuspended in 0.05% SDS to lyse cells.…”

Section: Methodsmentioning

confidence: 99%

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Influence of polyhedrin and host cell on the polyhedra morphology of Autographa californica nucleopolyhedrovirus

Woo

Ahn

2006

Appl. Entomol. Zool.

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A substitution of the polyhedrin of Autographa californica nucleopolyhedrovirus (AcNPV) with that of Spodoptera exigua NPV (SeNPV) and Bombyx mori NPV, respectively, resulted in morphological changes of the polyhedra. Morphological features and quantity of polyhedra of recombinant AcNPVs were examined and compared in three cell lines: Spodoptera frugiperda (Sf9), S. exigua (Se301) and Trichoplusia ni (Hi5) cells. The shape of the polyhedra produced by each virus did not change significantly according to the cell line. The size of the polyhedra differed, however, with the largest in Se301 cells, followed by Hi5 and Sf9 cells in decreasing order. The yield of polyhedra and the appearance of polyhedra-positive cells in each cell line also showed significant differences. Especially notable was the change of polyhedra shapes from irregular to regular in the recombinant AcNPV containing SeNPV polyhedrin in all cell lines. These results suggest that the shape and uniformity of polyhedra depend on some viral gene other than the polyhedrin gene. It is also suggested that there is a host cell factor that is responsible for the determination of polyhedra size.

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Section: Introductionmentioning

confidence: 74%

Section: Introductionmentioning

confidence: 85%

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Influence of polyhedrin and host cell on the polyhedra morphology of Autographa californica nucleopolyhedrovirus

Woo

Ahn

2006

Appl. Entomol. Zool.

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“…However, the formation of any kind of viral polyhedra seems to depend on specific interactions between molecules of polyhedrin itself ( Carstens et al, 1992 andCheng et al, 1998) and polyhedrin with host cell and viral factors ( Woo et al, 1998). Cheng et al (1998) replaced the polyhedrin gene of AcMNPV, whose occlusion bodies (OBs) are polyhedral, by the polyhedrin gene of Thysanoplusia orichalcea nucleopolyhedrovirus (ThorNPV), which produces tetrahedral OBs, and showed that tetrahedral polyhedra with properly occluded virions were produced.…”

Section: Discussionmentioning

confidence: 99%

“…However, the polyhedra morphology seems to take into account not only polyhedrin interactions with other viral and or host cell proteins (Woo et al, 1998), but its own amino acid sequence (Carstens et al, 1992, Cheng et al, 1998and Hu et al, 1999.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a new Autographa californica multiple nucleopolyhedrovirus (AcMNPV) polyhedra mutant

et al. 2009

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Characterization of a new Autographa californica multiple nucleopolyhedrovirus (AcMNPV) polyhedra mutantBergmann Morais Ribeiro Ana Paula Montenegro Generino Cláudia Natércia Lima Acacio Evanguedes Kalapothakis Sônia Nair Báo AbstractIn the very late phase of baculovirus infection, virions are occluded in a crystalline matrix called polyhedra, which is mainly composed of polyhedrin. This protein is highly conserved among baculoviruses and changes in its amino acid sequence may lead to mutant polyhedra. During the purification of an AcMNPV recombinant virus, a mutant virus was isolated. Structural and ultrastrutural analysis by light and transmission electron microscopy (TEM) of insect cells infected with this mutant virus did not show polyhedra formation and differed from the wild-type infection by the presence of a proteinaceous mass dispersed in the cytoplasm and nucleus of the infected cells, which was confirmed by immunogold labelling to be polyhedrin. The polyhedrin gene was amplified by PCR and sequenced. The only change observed was the substitution of a G to a T at the nucleotide +352, which resulted in a Val to Phe change. A recombinant virus was constructed by transferring the mutant gene into a polyhedrin negative virus. The phenotype of this recombinant virus was the same as the mutant one, confirming that this single mutation alone was responsible for the mutant phenotype.

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Abnormal Formation of Polyhedra Resulting from a Single Mutation in the Polyhedrin Gene of Autographa californica Multicapsid Nucleopolyhedrovirus

Lin¹,

Zhong

Wang³

2000

Journal of Invertebrate Pathology

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The morphology of the polyhedra of a host range-expanded recombinant baculovirus and its parents

Cited by 19 publications

References 9 publications

Influence of polyhedrin and host cell on the polyhedra morphology of Autographa californica nucleopolyhedrovirus

Influence of polyhedrin and host cell on the polyhedra morphology of Autographa californica nucleopolyhedrovirus

Characterization of a new Autographa californica multiple nucleopolyhedrovirus (AcMNPV) polyhedra mutant

Abnormal Formation of Polyhedra Resulting from a Single Mutation in the Polyhedrin Gene of Autographa californica Multicapsid Nucleopolyhedrovirus

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