The Motility of Monomeric and Dimeric Variants of Eg5 studied in the Presence of the Kinesin-5-specific Inhibitor Monastrol

Abstract

likely to undergo exocytosis than those without such associations. When endocytosis was inhibited with Dynasore (inhibitor of dynamin 1 and 2), membrane p/s changes following fusion were long-lived (10s of seconds). This is consistent with endocytosis occuring at the site of exocytosis. These experiments are the first to directly visualize plasma membrane changes occuring with secretion. They reveal an association of the granule and plasma membrane for seconds before exocytosis and provide direct evidence for maintained curvature of the plasma membrane after exocytosis.