The mouse pattern electroretinogram

Purpose: To compare amblyopic eyes and other eyes of the unilaterally amblyopic patients in the terms of pattern visual evoked potentials (pVEP), pattern electroretinogram (pERG) and flash electroretinogram(fERG) tests. Methods:This study was performed at Ankara Numune Training and Research Hospital between November-2015 and August-2016. Forty-one patients above the age of 15 with anisometropic amblyopia were evaluated for their amblyopic and other eyes. The patients were tested by Metrovision brand monpack model visual electrophysiology device for pVEP, fERG and pERG tests. Mean latance and amplitudes were examined statistically.Results: A statistically significant difference was found in decrease of P100 amplitude (p<0.05) and increase of P100 latency (p<0.01) of amblyopic eyes in all 5 patterns pVEP recorded. In pERG results P50 and N95 wave amplitudes were decreased in amblyopic eyes (p<0.01) but there was no statistical difference in latency period between amblyopic eyes and other eyes (p>0.05). In fERG results, rod response b wave amplitudes was lower and latency was increased in amblyopic eyes (p<0.01). However cone responses were no statistical difference in amblyopic and other eyes (p>0.05). Conclusion:According to our results, amblyopia is not only a cortical pathology. Also cortico-retinal pathologies that can not be detected by routine opthalmologic examination may accompany amblyopia. pERG, fERG and pVEP are objective methods for diagnosis and follow up of amblyopic patients and valuable guides for clinicians.

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“…Porciatti et al searched in rats and found that pERG responses developed in parallel with pVEP results in postnatal period [31][32].…”

Section: Discussionmentioning

confidence: 99%

“…[21] On the other hand Guttob and his friends [29] and Hess et al [30] stated that PERG is normal in any type of amblyopia. This study was undertaken to investigate the effect of amblyopia on both the retinal and cortical pattern responses [31].…”

Section: Discussionmentioning

confidence: 99%

Electrophysiologic evaluation of amblyopia

Hamurcu¹,

Çelik²,

Saricaoğlu³

et al. 2017

Eye Care Vis

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“…Pattern electroretinography was measured as previously reported (22,23,89). Mice from our colony were shipped in a "masked" fashion to the University of Miami, Miami, Florida, USA.…”

Section: Methodsmentioning

confidence: 99%

Radiation treatment inhibits monocyte entry into the optic nerve head and prevents neuronal damage in a mouse model of glaucoma

et al. 2012

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“…Mitochondrial mCherry fluorescence was visualized using a Heidelberg laser-scanning ophthalmoscope outfitted with special filters for red or green fluorescence. PERGs were performed according to previous reports (49). Averaged PERGs were automatically analyzed to evaluate the major positive and negative waves with Sigma Plot (Systat Software Inc.).…”

Section: Methodsmentioning

confidence: 99%

Consequences of zygote injection and germline transfer of mutant human mitochondrial DNA in mice

Koilkonda

Chou

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Considerable evidence supports mutations in mitochondrial genes as the cause of maternally inherited diseases affecting tissues that rely primarily on oxidative energy metabolism, usually the nervous system, the heart, and skeletal muscles. Mitochondrial diseases are diverse, and animal models currently are limited. Here we introduced a mutant human mitochondrial gene responsible for Leber hereditary optic neuropathy (LHON) into the mouse germ line using fluorescence imaging for tissue-specific enrichment in the target retinal ganglion cells. A mitochondria-targeted adeno-associated virus (MTS-AAV) containing the mutant human NADH ubiquinone oxidoreductase subunit 4 (ND4) gene followed by mitochondrial-encoded mCherry was microinjected into zygotes. Female founders with mCherry fluorescence on ophthalmoscopy were backcrossed with normal males for eight generations. Mutant human ND4 DNA was 20% of mouse ND4 and did not integrate into the host genome. Translated human ND4 protein assembled into host respiratory complexes, decreasing respiratory chain function and increasing oxidative stress. Swelling of the optic nerve head was followed by progressive demise of ganglion cells and their axons, the hallmarks of human LHON. Early visual loss that began at 3 mo and progressed to blindness 8 mo after birth was reversed by intraocular injection of MTS-AAV expressing wild-type human ND4. The technology of introducing human mitochondrial genes into the mouse germ line has never been described, to our knowledge, and has implications not only for creating animal models recapitulating the counterpart human disorder but more importantly for reversing the adverse effects of the mutant gene using gene therapy to deliver the wild-type allele.gene therapy | adeno-associated virus | mitochondria | Leber hereditary optic neuropathy | blindness

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The mouse pattern electroretinogram

Cited by 99 publications

References 61 publications

Electrophysiologic evaluation of amblyopia

Electrophysiologic evaluation of amblyopia

Radiation treatment inhibits monocyte entry into the optic nerve head and prevents neuronal damage in a mouse model of glaucoma

Consequences of zygote injection and germline transfer of mutant human mitochondrial DNA in mice

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