“…Mainly two processes determine the changes in the phenolic composition: the first one involves enzymatic reactions and occurs mostly at the early stages of winemaking, while, in the second one, the chemical reactivity of the phenolic compounds plays an important role, beginning in the winemaking process and continues during aging (Cheynier, Moutounet & Sanri-Manchado, 2000;Cheynier & Ricardo Da Silva, 1991;Sarni-Manchado, Cheynier, & Moutounet, 1997). During both phases, polyphenols are involved in several chemical reactions influencing the color, the color stability, the body and the astringency of the wine (Gonzáles-Manzano, Dueñas, Rivas-Gonzalo, Escribano-Bailón, & Santos-Buelga, 2009;Vidal et al, 2003;Vidal et al, 2004;Fontoin, Saucier, Teissedre, & Glories, 2008). Given that the polyphenolic fraction is present in wines in very low concentration, the solid phase extraction (SPE) over C18 columns has been proved to be a powerful tool for the isolation, purification and concentration of these molecular compounds from the wine matrix prior the analytical investigation (Briviba, Pan, & Rechkemmer, 2002;Edelmann, Diewok, Schuster, & Lendl, 2001).…”