The mRNA decapping protein 2 (DCP2) is a major regulator of developmental events in Drosophila—insights from expression paradigms

Kunar, Rohit; Roy, Jagat Kumar

doi:10.1007/s00441-021-03503-x

Cited by 3 publications

(1 citation statement)

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“…They are critical for regulating the homeostasis of cellular mRNAs levels and play crucial roles in numerous cellular and life processes ( 2 ). In humans and various model systems, decapping enzymes are involved in cell migration, development, and carcinogenesis ( 3 – 7 ). The enzymatic activity of decapping enzymes lies in the Nudix motif that hydrolyzes nucleoside diphosphate linked to other moieties ( 8 ).…”

Section: Introductionmentioning

confidence: 99%

A Poxvirus Decapping Enzyme Colocalizes with Mitochondria To Regulate RNA Metabolism and Translation and Promote Viral Replication

Cao

Molina

Cantu

et al. 2022

mBio

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Section: Introductionmentioning

confidence: 99%

A Poxvirus Decapping Enzyme Colocalizes with Mitochondria To Regulate RNA Metabolism and Translation and Promote Viral Replication

Cao

Molina

Cantu

et al. 2022

mBio

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Rab11 regulates mitophagy signaling pathway of Parkin and Pink1 in the Drosophila model of Parkinson's disease

Rai

Roy

2022

Biochemical and Biophysical Research Communications

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A poxvirus decapping enzyme localizes to mitochondria to regulate RNA metabolism and translation, and promote viral replication

Cao

Molina

Cantu

et al. 2021

Preprint

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Decapping enzymes remove the five-primer cap of eukaryotic mRNA, leading to accelerated RNA decay. They are critical in regulating RNA homeostasis and play essential roles in many cellular and life processes. They are encoded in many organisms and viruses, including vaccinia virus, which was used as the vaccine to eradicate smallpox. Vaccinia virus encodes two decapping enzymes, D9 and D10, that are necessary for efficient viral replication and pathogenesis. However, the underlying molecular mechanism regulating vaccinia decapping enzyme functions is still largely elusive. Here we demonstrated that vaccinia D10 localized almost exclusively to mitochondria that are highly mobile cellular organelles, providing an innovative mechanism to concentrate D10 locally and mobilize it to efficiently decap mRNAs. As mitochondria were barely present in viral factories, where viral transcripts are produced, suggesting that mitochondrial localization provides a spatial mechanism to preferentially decap cellular mRNAs over viral mRNAs. We identified three amino acids responsible for D10 mitochondrial localization. Loss of mitochondrial localization significantly impaired viral replication, reduced D10 ability to resolve RNA five-primer cap aggregation during infection, diminished D10 gene expression shutoff and mRNA translation promotion abilities.

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The mRNA decapping protein 2 (DCP2) is a major regulator of developmental events in Drosophila—insights from expression paradigms

Cited by 3 publications

References 50 publications

A Poxvirus Decapping Enzyme Colocalizes with Mitochondria To Regulate RNA Metabolism and Translation and Promote Viral Replication

A Poxvirus Decapping Enzyme Colocalizes with Mitochondria To Regulate RNA Metabolism and Translation and Promote Viral Replication

Rab11 regulates mitophagy signaling pathway of Parkin and Pink1 in the Drosophila model of Parkinson's disease

A poxvirus decapping enzyme localizes to mitochondria to regulate RNA metabolism and translation, and promote viral replication

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