The mRNA derived MalH sRNA contributes to alternative carbon source utilization by tuning maltoporin expression in E. coli

Iosub, Ira Alexandra; Marchioretto, Marta; Nues, Rob W. van; McKellar, Stuart; Viero, Gabriella; Granneman, Sander

doi:10.1080/15476286.2020.1827784

Cited by 19 publications

(12 citation statements)

References 79 publications

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“…Nitrate is then reduced in nitrite by NarG, and to limit nitrite toxicity, NarS is transcribed together with narK to reduce the expression of nirC encoding the nitrite transporter (Wang et al, 2020). The sRNA MalH derived from the processing of the 3′UTR of the polycistron malEFG encoding the maltose ABC transporter stimulates the use of alternative carbon sources in the presence of maltose (Bar et al, 2021;Iosub et al, 2021).…”

Section: The Dual Ribonuclease J1/j2 Generates the 3′utr-derived Rsag Srnamentioning

confidence: 99%

The 3′UTR‐derived sRNA RsaG coordinates redox homeostasis and metabolism adaptation in response to glucose‐6‐phosphate uptake in Staphylococcus aureus

Desgranges

Barrientos

Herrgott

et al. 2021

Molecular Microbiology

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Staphylococcus aureus is usually described as an extracellular opportunistic pathogen, infecting a wide range of organs and tissues.However, it also invades and replicates in various phagocytic or nonphagocytic host cells (Hamza & Li, 2014). To be successful as a pathogen, this bacterium needs to adapt to the hostile environment of the host and must acquire imposed nutriments for its survival.Consequently, the staphylococcal genome encodes several transporters for metabolites (sugars, metals, amino acids, etc.), which are

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Section: The Dual Ribonuclease J1/j2 Generates the 3′utr-derived Rsag Srnamentioning

confidence: 99%

The 3′UTR‐derived sRNA RsaG coordinates redox homeostasis and metabolism adaptation in response to glucose‐6‐phosphate uptake in Staphylococcus aureus

Desgranges

Barrientos

Herrgott

et al. 2021

Molecular Microbiology

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“…In contrast to Gram-positive bacteria, for which relatively few 3′ UTR-derived sRNAs have been described so far, Gram-negative organisms appear to be replete with these regulatory sRNA species that are released from mRNAs by endonucleolytic cleavage ( 5 , 50 – 52 ). In the Gram-negative pathogen Vibrio cholerae , the 3′ UTR sRNA OppZ is encoded at the 3′ end of the oppABCDF operon (encoding an oligopeptide transporter).…”

Section: Processed 3′ Utrs That Act In Trans (Type II 3′ Utr Srnas)mentioning

confidence: 99%

Burning the Candle at Both Ends: Have Exoribonucleases Driven Divergence of Regulatory RNA Mechanisms in Bacteria?

Mediati

Lalaouna

Tree

2021

mBio

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“…RhlS is derived from its 5 UTR of rhII and is required for the production of normal levels of AHL [55]. In E. coli, MalH is derived from the 3 UTR of the maltose uptake operon malEFG and contributes to alternative carbon source utilization by affecting maltoporin expression [56]. The product of argR and the 3 UTR-derived ArgX, both regulate the arginine deiminase pathway in Lactococcus lactis [57].…”

Section: Discussionmentioning

confidence: 99%

Maturation of UTR-Derived sRNAs Is Modulated during Adaptation to Different Growth Conditions

Spanka

Klug

2021

IJMS

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Small regulatory RNAs play a major role in bacterial gene regulation by binding their target mRNAs, which mostly influences the stability or translation of the target. Expression levels of sRNAs are often regulated by their own promoters, but recent reports have highlighted the presence and importance of sRNAs that are derived from mRNA 3′ untranslated regions (UTRs). In this study, we investigated the maturation of 5′ and 3′ UTR-derived sRNAs on a global scale in the facultative phototrophic alphaproteobacterium Rhodobacter sphaeroides. Including some already known UTR-derived sRNAs like UpsM or CcsR1-4, 14 sRNAs are predicted to be located in 5 UTRs and 16 in 3′ UTRs. The involvement of different ribonucleases during maturation was predicted by a differential RNA 5′/3′ end analysis based on RNA next generation sequencing (NGS) data from the respective deletion strains. The results were validated in vivo and underline the importance of polynucleotide phosphorylase (PNPase) and ribonuclease E (RNase E) during processing and maturation. The abundances of some UTR-derived sRNAs changed when cultures were exposed to external stress conditions, such as oxidative stress and also during different growth phases. Promoter fusions revealed that this effect cannot be solely attributed to an altered transcription rate. Moreover, the RNase E dependent cleavage of several UTR-derived sRNAs varied significantly during the early stationary phase and under iron depletion conditions. We conclude that an alteration of ribonucleolytic processing influences the levels of UTR-derived sRNAs, and may thus indirectly affect their mRNA targets.

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The mRNA derived MalH sRNA contributes to alternative carbon source utilization by tuning maltoporin expression in E. coli

Abstract: Granneman (2020): The mRNA derived MalH sRNA contributes to alternative carbon source utilization by tuning maltoporin expression in E. coli, RNA Biology,

Cited by 19 publications

References 79 publications

The 3′UTR‐derived sRNA RsaG coordinates redox homeostasis and metabolism adaptation in response to glucose‐6‐phosphate uptake in Staphylococcus aureus

The 3′UTR‐derived sRNA RsaG coordinates redox homeostasis and metabolism adaptation in response to glucose‐6‐phosphate uptake in Staphylococcus aureus

Burning the Candle at Both Ends: Have Exoribonucleases Driven Divergence of Regulatory RNA Mechanisms in Bacteria?

Maturation of UTR-Derived sRNAs Is Modulated during Adaptation to Different Growth Conditions

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