The mRNA levels of thyrotropin receptor, thyroglobulin and thyroid peroxidase in neoplastic human thyroid tissues

Ohta, Kazuyasu; Endo, Toyoshi; Onaya, Toshimasa

doi:10.1016/0006-291x(91)91540-s

Cited by 92 publications

(62 citation statements)

References 11 publications

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“…Wollman (1963) has also shown some tendency towards progressive dedifferentiation among the more differentiated thyroid tumours in rats. Differentiated human thyroid carcinomas, however, express Tg, TPO and TSH receptor mRNA to varying degrees (Brabant et al, 1991;Ohta et al, 1991). Similar to FRTL-5 cells and some of previously reported mutant clones (Tramontano et al, 1986a;Endo et al, 1990), FRTC cells produced cAMP and Tg in a dose-dependent manner when stimulated with TSH.…”

Section: Discussionsupporting

confidence: 67%

“…The basis of this discrepancy, however, is unknown. In thyroid papillary or follicular carcinoma tissues, TSH receptor mRNA levels have been reported to vary from normal to markedly decreased (Brabant et al, 1991;Ohta et al, 1991). TSH receptor mRNA levels were high in benign thyroid tumours but not detectable in anaplastic cancers, indicating that the expression of TSH receptor mRNA levels may be dependent on the differentiation of thyroid cells.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, human thyroid cancers are often characterized by well-differentiated features that include Tg production and expression of Tg, thyroid peroxidase (TPO) and TSH receptor mRNA. (Brabant et al, 1991;Ohta et al, 1991).…”

mentioning

confidence: 99%

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Transplantable rat thyroid cancer cell line FRTC transformed with muramyl dipeptide

Iitaka

Fukasawa²,

Kitahama³

et al. 1997

Br J Cancer

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Section: Discussionsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 99%

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Transplantable rat thyroid cancer cell line FRTC transformed with muramyl dipeptide

Iitaka

Fukasawa²,

Kitahama³

et al. 1997

Br J Cancer

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“…In neoplastic thyroid tissue, however, its expression is considerably lower (Ohta et al, 1991;Hoang-Vu et al, 1992;Lazar et al, 1999;Ringel et al, 2001).…”

Section: Thyroglobulin Mrnamentioning

confidence: 97%

Detection of circulating Tg-mRNA in the follow-up of papillary and follicular thyroid cancer: how useful is it?

et al. 2004

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To investigate the usefulness of thyroglobulin mRNA (Tg-mRNA) detection in peripheral blood in the follow-up of papillary and follicular (differentiated) thyroid cancer, a literature study was performed. Both evidence for and evidence against the usefulness of Tg-mRNA detection were found. Also, evidence for the expression of Tg-mRNA in cells other than normal or neoplastic thyroid follicular cells was found. It is concluded that currently Tg-mRNA detection is not a useful tool in the follow-up of differentiated thyroid carcinoma, but that the concept of using RT -PCR measurements during follow-up still warrants further research.

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“…In the literature, species-specific responses of TSH-R mRNA expression to TSH exposure have been reported: down-regulation in rat thyrocytes (Akamizu et al, 1990; and the present findings), a slight down-regulation in dog thyrocytes and no marked down-regulation in human thyrocytes . Significantly reduced TSH-R gene expression has also been found in neoplastic human thyroid tissues in conjunction with reduced thyroglobulin gene expression (Ohta et al, 1990;Elisei et al, 1994). However, several laboratories have shown that functional TSH binding is, in general, not different between normal and neoplastic human thyroid tissues (Karlsson and Dahlberg, 1979;Matsuo et al, 1993 Civitareale et al, 1993;Shimura et al, 1994).…”

mentioning

confidence: 99%

Dissociation of thyrotropin receptor function and thyrotropin dependency in rat thyroid tumour cell lines derived from FRTL-5

Kallen

Coes²,

Grafhorst³

et al. 1996

Br J Cancer

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Summary Spontaneously transformed somatic thyrocyte mutants, FRTL-5/TA and FRTL-5/TP, are thyrotropin (TSH) independent for growth and show loss of the thyroid-specific phenotype, with absent thyroglobulin and thyroid peroxidase gene expression. To investigate the role of TSH-receptor (TSH-R) activation in rat thyroid growth and function, binding of TSH and TSH-induced cAMP production were measured in intact cells under identical assay conditions. TSH binding did not differ in terms of affinity and receptor number and presence of 5.6 kb and 3.3 kb mRNA rat TSH-R transcripts was determined in all variants. By contrast, basal cAMP was I1-fold lower in FRTL-5/TA and 6-fold lower in FRTL-5/TP than in wild-type FRTL-5 (1.1+0.4; P<0.01). Maximal cAMP production was similar between wild-type and cell variants and stimulation by bovine, rat and recombinant human TSH revealed normal activation patterns. Therefore, a dissociation was present between the loss of TSH control on growth and function, and the presence of a normally functioning TSH-R. Subsequent to TSH incubation FRTL-5/TP and FRTL-5/TA cells showed a different expression pattern of TSH-R and the proto oncogenes c-myc and fos than FRTL-5 wildtype. The data indicated that the cause of the TSH-independency is located down-stream of the cAMP cascade, influencing genes that control the expression of cell cycle-related proto-oncogenes and thyroid-specific genes.

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The mRNA levels of thyrotropin receptor, thyroglobulin and thyroid peroxidase in neoplastic human thyroid tissues

Cited by 92 publications

References 11 publications

Transplantable rat thyroid cancer cell line FRTC transformed with muramyl dipeptide

Transplantable rat thyroid cancer cell line FRTC transformed with muramyl dipeptide

Detection of circulating Tg-mRNA in the follow-up of papillary and follicular thyroid cancer: how useful is it?

Dissociation of thyrotropin receptor function and thyrotropin dependency in rat thyroid tumour cell lines derived from FRTL-5

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