2012
DOI: 10.1371/journal.pone.0045156
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The mTOR Inhibitor Rapamycin Has Limited Acute Anticonvulsant Effects in Mice

Abstract: ObjectiveThe mammalian target of rapamycin (mTOR) pathway integrates signals from different nutrient sources, including amino acids and glucose. Compounds that inhibit mTOR kinase activity such as rapamycin and everolimus can suppress seizures in some chronic animal models and in patients with tuberous sclerosis. However, it is not known whether mTOR inhibitors exert acute anticonvulsant effects in addition to their longer term antiepileptogenic effects. To gain insights into how rapamycin suppresses seizures,… Show more

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Cited by 50 publications
(33 citation statements)
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“…of rapamycin (?Rap) or vehicle (?veh) for 7 days beginning 30 min before soman exposure. This dose of rapamycin has previously shown to dramatically suppress mTOR activity in the cortex and hippocampus (Hartman et al 2012). …”
Section: Drug Treatmentsmentioning
confidence: 98%
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“…of rapamycin (?Rap) or vehicle (?veh) for 7 days beginning 30 min before soman exposure. This dose of rapamycin has previously shown to dramatically suppress mTOR activity in the cortex and hippocampus (Hartman et al 2012). …”
Section: Drug Treatmentsmentioning
confidence: 98%
“…Rapamycin was diluted to a final concentration of 4 % ethanol and 1 mg/ml rapamycin in a vehicle solution containing 5 % Tween 80 (Sigma), 5 % PEG 400 (low molecular weight grade of polyethylene glycol) (Sigma) immediately prior to use as previously described (Buckmaster and Lew 2011;Hartman et al 2012). Both rapamycin-and vehicle-treated rats received the same concentration of ethanol (4 %).…”
Section: Drug Treatmentsmentioning
confidence: 99%
“…A rapamycin stock solution was prepared by dissolving 11.25 mg/mL rapamycin in 99.5% ethanol. To prepare the rapamycin solution (0.45 mg rapamycin/mL (4% ethanol)) used for injection, the stock solution was diluted with 5% (w/v) polyethylene glycol-400 (PEG) and 5% (w/v) Tween 80 13) immediately prior to use. The solution without rapamycin was used as the corresponding Veh.…”
mentioning
confidence: 99%
“…The dose of rapamycin chosen was able to completely inhibit mTORC1 activity. 13) Three hours after injection of Rap or Veh, each group was subdivided into two groups: saline (Veh/Sal and Rap/Sal) and leucine (Veh/Leu and Rap/Leu) groups (n = 6-8 per group). These subgroups were orally administered saline or leucine (0.45 g/kg BW 14) ) respectively.…”
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confidence: 99%
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