The MUC6 secretory mucin gene is expressed in a wide variety of epithelial tissues

Bartman, Allen E.; Buisine, MarieâPierre; Aubert, JeanâPierre; Niehans, Gloria A.; Toribara, Neil W.; Kim, Young S.; Kelly, Eric J.; Crabtree, Jean E.; Ho, Samuel B.

doi:10.1002/(sici)1096-9896(199812)186:4<398::aid-path192>3.3.co;2-o

Cited by 27 publications

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“…In human, MUC6 was shown in mucous neck cells of the fundus, antral-type glands of the antrum and cardia, and Brunner's glands of the duodenum (de Bolos et al 1995;Ho et al 1995). Furthermore, in stomach, surface mucous cells throughout the entire stomach exclusively expressed MUC5AC mucin protein, whereas mucous neck cells and antral gland-type mucous cells only express MUC6 mucin (Bartman et al 1998;de Bolos et al 1995;Ho et al 1995). However, the presence of MUC6 protein in the adherent gastric mucous layer has been shown, with a segregation of MUC5AC and MUC6 within the mucous gel (Ho et al 2004).…”

Section: Distribution Of Muc6 Protein In Normal Mouse Tissuesmentioning

confidence: 99%

“…A stronger reactivity of CP4 was detected in centroacinar cells of pancreas of Cftr¡/¡ mice compared with their wild-type littermates. Nuclei are counterstained with propidium iodide Expression of Muc6 in pancreas of Cftr+/+ and Cftr¡/¡ mice In human, the MUC6 mucin is abundant in centroacinar cells and pancreatic ductules (Bartman et al 1998;Reid et al 1997). Moreover, MUC6 is a signiWcant component of material obstructing the small intralobular ducts in the pancreas of cystic Wbrosis patients (Reid and Harris 1998).…”

Section: Distribution Of Muc6 Protein In Normal Mouse Tissuesmentioning

confidence: 99%

“…The MUC6 mRNA is detected by in situ hybridisation in a variety of human tissues through foetal development (Reid et al 1997;Harris 1998, 1999). Moreover, MUC6 is one of the major high-molecular weight secreted mucins in postnatal and adult duct pancreas (Bartman et al 1998;Reid et al 1997) and has been shown to contribute to the material that obstructs the pancreatic ducts of cystic Wbrosis patients (Reid et al 1997). Pancreatic exocrine insuYciency occurs in 85% of individuals with cystic Wbrosis (Snouwaert et al 1992).…”

Section: Introductionmentioning

confidence: 99%

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The characterization of the first anti-mouse Muc6 antibody shows an increased expression of the mucin in pancreatic tissue of Cftr-knockout mice

Gouyer

Leir

Tétaert

et al. 2010

Histochem Cell Biol

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Gel-forming mucins are large high-molecular weight secreted O-glycoproteins responsible for the gel-properties of the mucus blanket. Five orthologous gel-forming mucins have been cloned in human and mouse. Among them, the mucin MUC6 has been less studied, particularly in rodents and no anti rodent-Muc6 antibody has been reported yet. In order to further study Muc6 in mice, our aims were to obtain a specific Muc6 antibody, to validate it and to test it in Cftr deficient mice. A polyclonal serum named CP4 was isolated from a rabbit immunized by a mouse Muc6 peptide. In Western blot experiments, the antibody detected a high-molecular weight molecule secreted by the gastric tissue. Using immunohistochemistry, we showed that the antibody reacted strongly with deep glands of duodenum and ileum and mucous neck cells of gastric body. CP4 also recognized Muc6 protein secreted at the surface of the stomach and renal collecting tubules. The centroacinar cells of pancreatic tissue also reacted with the antibody. Cftr-/- mice showed a higher expression of Muc6 at both protein and RNA levels compared with their control Cftr+/+ littermates suggesting that as in the human disease, Muc6 may contribute to the formation of materials that block pancreatic acini and ducts in mouse models of cystic fibrosis. The rabbit anti-mouse Muc6 polyclonal antibody seems highly specific to the mouse mucin and will be useful to study pancreatic pathology in cystic fibrosis.

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Section: Distribution Of Muc6 Protein In Normal Mouse Tissuesmentioning

confidence: 99%

Section: Distribution Of Muc6 Protein In Normal Mouse Tissuesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The characterization of the first anti-mouse Muc6 antibody shows an increased expression of the mucin in pancreatic tissue of Cftr-knockout mice

Gouyer

Leir

Tétaert

et al. 2010

Histochem Cell Biol

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“…In normal stomach, MUC1 is detected in mucous cells of the surface epithelium and neck regions of the gastric antrum, as well as in pyloric glands and oxynthic glands of the body region [23,24,41]. MUC5AC is highly expressed in foveolar epithelium of both antrum and body [2,8,24,39], whereas MUC6 protein expression is limited to mucous neck cells of the body and pyloric glands of the antrum [6,8,10,11,24,42]. In gastric carcinoma, the mucin expression is heterogeneous.…”

Section: Introductionmentioning

confidence: 99%

MUC5B expression in gastric carcinoma: relationship with clinico-pathological parameters and with expression of mucins MUC1, MUC2, MUC5AC and MUC6

et al. 2004

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Previous studies have shown that mucin expression can be used to evaluate differentiation patterns of gastric carcinoma: MUC5AC expression is associated with diffuse type and early gastric carcinomas, and MUC2 expression is associated with mucinous gastric carcinomas. The role played by MUC5B in the evaluation of differentiation and biological behaviour of gastric carcinoma is largely unknown. Our aim was to characterise the pattern of expression of mucins MUC1, MUC2, MUC5AC, MUC5B and MUC6 in a series of 50 gastric carcinomas to evaluate whether MUC5B expression was associated with the clinico-pathological characteristics of the cases and/or with the co-expression of other mucins. A panel of six monoclonal antibodies (HMFG1, SM3, PMH1, CLH2, EU-MUC5Ba and CLH5) was used to determine the expression of mucins (MUC1, MUC1 underglycosylated form, MUC2, MUC5B, MUC5AC and MUC6, respectively) using immunohistochemistry. Cases were considered positive if more than 5% of the cells expressed immunoreactivity for the several mucins evaluated. Our results showed that: (a) expression of MUC5B was observed in 11 cases (22.0%) and was associated with the "unclassified" histological type of gastric carcinoma according to Laurén ( P = 0.03) and with the absence of venous invasion ( P = 0.02); (b) in this series, MUC5B expression had no impact on survival of patients with gastric carcinoma; (c) the expression of MUC5B was associated with the co-expression of MUC5AC ( P = 0.02) and (d) none of the cases with the so-called complete intestinal phenotype of mucin expression expressed MUC5B.

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“…MUC1 is detected in mucous cells of the surface epithelium and neck region of the gastric antrum, as well as in pyloric glands and oxynthic glands of the body region [22,23,34]. MUC5AC is highly expressed in foveolar epithelium of both body and antrum [2,11,23,33], whereas MUC6 protein expression is limited to mucous neck cells of the body and pyloric glands of the antrum [4,7,11,23,37].…”

Section: Introductionmentioning

confidence: 99%

Mucins MUC1, MUC2, MUC5AC and MUC6 expression in the evaluation of differentiation and clinico-biological behaviour of gastric carcinoma

et al. 2001

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Normal gastric mucosa expresses MUC1 and MUC5AC in foveolar epithelium and MUC6 in mucous neck cells of the body and deep glands of the antrum. Several studies have reported aberrant expression of an under-glycosylated form of MUC1, decreased expression of "gastric" mucins and aberrant expression of "non-gastric" mucins in gastric carcinoma. In this study, we analysed the expression profile of mucins MUC1, MUC2, MUC5AC and MUC6 in 94 gastric carcinomas using immunohistochemistry. Our results showed that: (1) mucin expression is associated with tumour type (MUC5AC with diffuse and infiltrative carcinomas and MUC2 with mucinous carcinomas) but not with the clinico-biological behaviour of the tumours; and (2) mucin expression is associated with tumour location (MUC5AC with antrum carcinomas and MUC2 with cardia carcinomas), indirectly reflecting differences in tumour differentiation according to tumour location.

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The MUC6 secretory mucin gene is expressed in a wide variety of epithelial tissues

Cited by 27 publications

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The characterization of the first anti-mouse Muc6 antibody shows an increased expression of the mucin in pancreatic tissue of Cftr-knockout mice

The characterization of the first anti-mouse Muc6 antibody shows an increased expression of the mucin in pancreatic tissue of Cftr-knockout mice

MUC5B expression in gastric carcinoma: relationship with clinico-pathological parameters and with expression of mucins MUC1, MUC2, MUC5AC and MUC6

Mucins MUC1, MUC2, MUC5AC and MUC6 expression in the evaluation of differentiation and clinico-biological behaviour of gastric carcinoma

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