The mucosal expression signatures of g-type lysozyme in turbot (Scophthalmus maximus) following bacterial challenge

Gao, Chengbin; Fu, Qiang; Zhou, Shun; Song, Lin; Ren, Yichao; Dong, Xiaoyu; Su, Baofeng; Li, Chao

doi:10.1016/j.fsi.2016.05.015

Cited by 42 publications

(14 citation statements)

References 35 publications

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“…Interestingly, in this study, the expression of lysozyme was significantly upregulated in CpG-treated group that survived after infection in the kidney and gill, while shows the opposite expression pattern in the intestine. This suggested that lysozyme has different expression patterns in different tissues, which is consistent with the study results reported by Gao et al (Gao et al 2016).…”

Section: Discussionsupporting

confidence: 92%

“…It is speculated that CpG-ODN 2007 is mainly recognized in these two organs where it may reduce the overexpression of IL-1β (Tassakka et al 2006). Lysozyme is considered as one of the key components for the innate immune response to pathogen infection with their strong antibacterial properties, and its expression level is considered an important index for monitoring fish immunity (Gao et al 2016). Interestingly, in this study, the expression of lysozyme was significantly upregulated in CpG-treated group that survived after infection in the kidney and gill, while shows the opposite expression pattern in the intestine.…”

Section: Discussionmentioning

confidence: 99%

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CpG-ODN 2007 protects zebrafish against Vibrio vulnificus-induced infection

Chen

Zhang²,

S³

et al. 2019

Preprint

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Vibrio vulnificus (V. vulnificus) is an aquatic pathogen that can cause primary sepsis and soft tissue infection. CpG oligodeoxynucleotides (CpG-ODN) are a type of essential immunomodulators, which can trigger or enhance immune responses in mammals, fish, and humans. In this study, we evaluated the effect of CpG-ODN 2007 as a potential immunostimulant for zebrafish infected with V. vulnificus (FJ03-X2). Fish injected with the CpG-ODN 2007 showed lower mortality rate compared with the fish that did not receive treatment. The survival rates of CpG-ODN 2007-treated group and PBS-treated group were 85% and 57.9%, respectively. In addition, our in vitro results demonstrated that CpG-ODN 2007 can effectively reduce the toxicity of V. vulnificus (FJ03-X2) to zebrafish embryonic fibroblast (ZF4) cells. Furthermore, we assessed immune-related genes expression patterns in FJ03-X2 infected zebrafish or ZF4 cells with and without CpG-ODN 2007 treatment, such as TLRs and IL-1β. To sum up, our data indicated that CpG-ODN 2007 protects zebrafish against Vibrio vulnificus induced infection.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

CpG-ODN 2007 protects zebrafish against Vibrio vulnificus-induced infection

Chen

Zhang²,

S³

et al. 2019

Preprint

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“…It is well documented that fish lysozyme possesses lytic activity against both Gram-positive bacteria and Gram-negative bacteria ( 41 ). The g-type lysozyme in turbot has been shown to play an important role in the defense against most bacterial infections ( 42 ), in particular, g-type lysozyme increased the disease resistance in the mucosal surfaces of fish ( 43 ). Therefore, the expression and activity of turbot g-type lysozyme in HKMs were analyzed in our study.…”

Section: Discussionmentioning

confidence: 99%

Short-Chain Fatty Acids Promote Intracellular Bactericidal Activity in Head Kidney Macrophages From Turbot (Scophthalmus maximus L.) via Hypoxia Inducible Factor-1α

Zhang

Liu

et al. 2020

Front. Immunol.

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Short-chain fatty acids (SCFAs) are mainly produced by microbiota through the fermentation of carbohydrates in the intestine. Acetate, propionate, and butyrate are the most abundant SCFA metabolites and have been shown to be important in the maintenance of host health. In this study, head kidney macrophages (HKMs) were isolated and cultured from turbots. We found that the antibacterial activity of HKMs was increased after these cells were incubated with sodium butyrate, sodium propionate or sodium acetate. Interestingly, our results showed that all three SCFAs enhanced the expression of hypoxia inducible factor-1 α (HIF-1α) in HKMs, and further study confirmed that butyrate augmented the oxygen consumption of these cells. Moreover, HIF-1α inhibition diminished the butyrate-promoted intracellular bacterial killing activity of macrophages, and SCFAs also raised the gene expression and activity of lysozymes in HKMs via HIF-1α signaling. In addition, our results suggested that butyrate induced HIF-1α expression and the bactericidal activity of HKMs through histone deacetylase inhibition, while G protein-coupled receptors did not contribute to this effect. Finally, we demonstrated that butyrate induced a similar response in the murine macrophage cell line RAW264.7. In conclusion, our results demonstrated that SCFAs promoted HIF-1α expression via histone deacetylase inhibition, leading to the enhanced production of antibacterial effectors and increased bacterial killing of macrophages.

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“…Turbot (S. maximus) is one of the extensively maricultured species in China, suffering from widespread disease outbreaks due to several pathogens, including Vibrio anguillarum, Streptococcus iniae and Edwardsiella tarda. In order to develop disease control and prevention measures, much efforts have been made to identify the immune genes in turbot, as well as their associate immune activities following infection [19][20][21][22][23]. The mucosal surfaces constitute the first immune barrier of fish host defense, a better understanding of mucosal barriers is urgent for aquaculture research to improve vaccine delivery via immersion or feeding [24].…”

Section: Introductionmentioning

confidence: 99%

Characterization and expression analysis of chitinase genes (CHIT1, CHIT2 and CHIT3) in turbot (Scophthalmus maximus L.) following bacterial challenge

Gao

Cai

Zhang

et al. 2017

Fish & Shellfish Immunology

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Chitinases are hydrolytic enzymes which have been employed to breakdown chitin coats of pathogenic microorganisms, thereby weaken the defense system of several pathogens and insects. In this regard, we identified the chitinase genes of turbot and characterized their expression patterns in mucosal tissues following Vibrio anguillarum and Streptococcus iniae challenge. In present study, transcripts of three chitinase genes (CHIT1, CHIT2 and CHIT3) were captured, as well as their protein structures and expression patterns following different bacterial infection were also characterized. The chitinases were widely expressed in all tested tissues with the highest expression levels of CHIT1 and CHIT2 in intestine, and CHIT3 in skin. Finally, these three genes showed different expression patterns following bacterial challenge. The significant quick induction of chitinases in mucosal surfaces against infection indicated their key roles to prevent pathogen attachment and entry in mucosal immunity. Functional studies should further characterize the chitinases and avail utilization of their function to increase the disease resistance in maintaining the integrity of the mucosal barriers against infection and facilitating the disease resistant family/strain selection in turbot.

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The mucosal expression signatures of g-type lysozyme in turbot (Scophthalmus maximus) following bacterial challenge

Cited by 42 publications

References 35 publications

CpG-ODN 2007 protects zebrafish against Vibrio vulnificus-induced infection

CpG-ODN 2007 protects zebrafish against Vibrio vulnificus-induced infection

Short-Chain Fatty Acids Promote Intracellular Bactericidal Activity in Head Kidney Macrophages From Turbot (Scophthalmus maximus L.) via Hypoxia Inducible Factor-1α

Characterization and expression analysis of chitinase genes (CHIT1, CHIT2 and CHIT3) in turbot (Scophthalmus maximus L.) following bacterial challenge

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