The Multi-Template Molecularly Imprinted Polymer Based on SBA-15 for Selective Separation and Determination of Panax notoginseng Saponins Simultaneously in Biological Samples

Abstract

Abstract:The feasible, reliable and selective multi-template molecularly imprinted polymers (MT-MIPs) based on SBA-15 (SBA-15@MT-MIPs) for the selective separation and determination of the trace level of ginsenoside Rb 1 (Rb 1 ), ginsenoside Rg 1 (Rg 1 ) and notoginsenoside R 1 (R 1 ) simultaneously from biological samples were developed. The polymers were constructed by SBA-15 as support, Rb 1 , Rg 1 , R 1 as multi-template, acrylamide (AM) as functional monomer and ethylene glycol dimethacrylate (EGDMA) as cross-linker. The new synthetic SBA-15@MT-MIPs were satisfactorily applied to solid-phase extraction (SPE) coupled with high performance liquid chromatography (HPLC) for the separation and determination of trace Rb 1 , Rg 1 and R 1 in plasma samples. Under the optimized conditions, the limits of detection (LODs) and quantitation (LOQs) of the proposed method for Rb 1 , Rg 1 and R 1 were in the range of 0.63-0.75 ng·mL −1 and 2.1-2.5 ng·mL −1 , respectively. The recoveries of R 1 , Rb 1 and Rg 1 were obtained between 93.4% and 104.3% with relative standard deviations (RSDs) in the range of 3.3-4.2%. All results show that the obtained SBA-15@MT-MIPs could be a promising prospect for the practical application in the selective separation and enrichment of trace Panax notoginseng saponins (PNS) in the biological samples.

Multifiber solid‐phase microextraction using different molecularly imprinted coatings for simultaneous selective extraction and sensitive determination of organophosphorus pesticides

“…Selectivity factor is defined by comparison of the peak areas using the multifiber SPME device with a NIP fiber. The values were 3.89, 3.49, 2.51, 3.1, and 2.95 for diazinon, parathion‐methyl, pirimiphos‐methyl, quinalphos, and isocarbophos, respectively, similar to those reported in some literature by multi‐template MIP‐SPE method . The results indicated that the multi‐MIP‐fibers possessed good selectivity to template molecules and their structural analogs.…”

Recent Advances and Future Prospects of Molecular Imprinting Polymers as a Recognition Sensing System for Food Analysis: A Review

“…After the stacking stage, the washing stage is intended to eliminate unwanted debasements from the cartridge, but the dissolvable elution must be updated by its ability to undermine contact between analysts and polymers. SPME has shown itself to be a normal device for its more points of interest, for example, effortlessness of activity, dissolvable less nature, and the accessibility of business strands [151][152][153]. In other methods, the matrix solid-phase dispersion is set up with olaquindox as the format, methacrylic corrosive as a utilitarian monomer, and EGDMA as the crosslinking specialist.…”

Strategies of molecular imprinting-based solid-phase extraction prior to chromatographic analysis

“…Sun and coworkers fabricated mt-MIPs by using SBA-15 as solid support, the components of ginsenoside Rb1 (Rb1), ginsenoside Rg1 (Rg1) and notoginsenoside R1 (R1) as templates, acrylamide as a functional monomer and EGDMA as cross-linker [180]. According to chemical structure and functional groups of templates, the ratio of Rb1 to Rg1 to R1 of 44:43:13 in ethanol porogen was selected for preparation of mt-MIPs.…”