The multi-xenobiotic resistance (MXR) efflux activity in hemocytes of Mytilus edulis is mediated by an ATP binding cassette transporter of class C (ABCC) principally inducible in eosinophilic granulocytes

Rioult, Damien; Pasquier, Jennifer; Boulangé-Lecomte, Céline; Poret, Agnès; Abbas, Imane; Marin, Matthieu; Minier, Christophe; Foll, Frank Le

doi:10.1016/j.aquatox.2013.11.012

Cited by 20 publications

(13 citation statements)

References 61 publications

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“…Specimens of P. serratus were collected with a hand-net from the intertidal rocky shore of Yport during low tide (49°44′ 27″ N, 0°18′ 35″ E), a known slightly polluted station according to ROCCH data records (Réseau d'Observation de la Contamination Chimique du littoral) and used as a reference site in our laboratory for biomonitoring studies in the Seine Bay (e.g. Rioult et al 2014). Prawns were brought to the laboratory within 1 h at the most in 30-Lplastic containers under oxygenation.…”

Section: Collection Of Prawns and Maintenance Of Animalsmentioning

confidence: 99%

Assessment of sperm quality in palaemonid prawns using Comet assay: methodological optimization

Erraud

Bonnard

Duflot

et al. 2017

Environ Sci Pollut Res

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The aim of this study was to adapt the Comet assay in spermatozoa of the marine prawn Palaemon serratus to use it as a marker of sperm quality. Indeed, due to the characteristics of their spermatozoa, the measurement of DNA integrity is one of the few markers which can be transferred to crustaceans to assess the quality of their semen. In the first step, the methods of collecting and maintaining spermatozoa were optimized. Cell survival was estimated during kinetics of preservation (i.e. 1, 2, 4 and 8 h) in various suspension media to define artificial seawater (ASW) as optimal. Several methods in the releasing of spermatozoa from the spermatophore of prawns were estimated with regard to their incidence both on the efficiency of extraction and the survival of cells. Pipetting up and down turned out to be the most successful and the least invasive technique. Secondly, the transfer of Comet assay was optimized by studying various times in both cell lysis (i.e. 1, 6, 18 h) and DNA denaturation (i.e. 15, 30 and 45 min), after in vitro exposure of spermatozoa to an H2O2 gradient as model genotoxicant. Results revealed that a minimum of 1 h in cell lysis and 15 min of DNA denaturation were sufficient to obtain valuable results, linked with a low compaction of DNA in spermatozoa of Palaemon sp. Finally, the sensitivity of P. serratus spermatozoa was assessed after in vitro exposures to model genotoxicants displaying various modes of interaction with DNA (i.e. UV-C, 13.3-79.5 J m; HO, 5-10 μM and MMS, 0.5-5 mM) and some environmental contaminants known or suspected to be genotoxic (i.e. cadmium and diuron, 0.015-1.5 μg L; carbamazepine, 0.1-10 μg L) for invertebrates. The low variability of the baseline level of DNA strand breaks recorded in controls highlighted the robustness of the method. P. serratus spermatozoa displayed significant DNA damage from the lowest doses tested for all model genotoxicants, but conversely, no genotoxic effect of tested environmental contaminants was observed. These results, which are discussed according to the protocol tested in the present study and the comparison with literature data, could suggest a difference in the response or sensitivity of spermatozoa to environmental genotoxicity between invertebrate species, and therefore the interest of Palaemonidae prawns in ecogenotoxicology. In conclusion, the present study underlines the potential of the Comet assay as a marker to assess the contamination impact on the sperm quality in Palaemonidae prawns in view to a potential application for in situ biomonitoring surveys.

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Section: Collection Of Prawns and Maintenance Of Animalsmentioning

confidence: 99%

Assessment of sperm quality in palaemonid prawns using Comet assay: methodological optimization

Erraud

Bonnard

Duflot

et al. 2017

Environ Sci Pollut Res

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“…The presence and activity of the MXR mechanism were also established in mussels, including Dreissena sp. (Cornwall et al, 1995;Smital et al, 2000;Pain and Parant 2003;Rioult et al, 2014). Alteration (activation or inhibition) of this cellular defence system may also serve as a biological indicator of environmental pollution (Pain and Parant 2007;Contardo-Jara and Wiegand 2008;Luckenbach and Epel, 2008;Ács et al, 2015;Jeong et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Neonicotinoid insecticides are potential substrates of the multixenobiotic resistance (MXR) mechanism in the non-target invertebrate, Dreissena sp.

et al. 2018

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Mussels are among the most frequently used invertebrate animals in aquatic toxicology to detect toxic exposure in the environment. The presence and activity of a cellular defence system, the multixenobiotic resistance (MXR) mechanism, was also established in these organisms.In isolated gill tissues of dreissenid mussels (D. bugensis) the MXR activity was assayed after treatment by commercially available insecticides (formulated products) which contain neonicotinoids as their active ingredients: Actara (thiamethoxam), Apacs (clothianidin), Calypso (thiacloprid) and Kohinor (imidacloprid), respectively. While applying the accumulation assay method, 0.5 μM rhodamine B was used as model substrate and 20 μM verapamil as model inhibitor of the MXR mechanism.In acute (in vitro) experiments when isolated gills were co-incubated in graded concentrations of insecticides and rhodamine B simultaneously, Calypso and Kohinor treatment resulted increasing rhodamine accumulation. Chemical analysis of gills in vitro incubated in insecticides demonstrated higher tissue concentrations of thiamethoxam, clothianidin and thiacloprid in the presence of verapamil suggesting that the active ingredients of Actara, Apacs and Calypso are potential substrates of the MXR mediated cellular efflux. In contrast, verapamil did significantly alter the accumulated imidacloprid concentrations in gills, suggesting that the active component of Kohinor is not transported by the MXR mechanism.Chronic (in vivo) exposures of the intact animals in lower, 1, 10 mg/L concentration of neonicotinoid products, resulted in a decreased level of both rhodamine accumulation and verapamil inhibition by the 12 th -14 th days of treatment. These results suggest an enhancement of MXR activity (chemostimulation), building up gradually in the animals exposed to Actara, Apacs and Kohinor, respectively.Neonicotinoid-type insecticides are generally considered as selective neurotoxins for insects, targeting the nicotinic type acetylcholine receptors (nAChRs) in their central nervous system. Our present results provide the first evidences that neonicotinoid insecticides are also able to alter the transmembrane transport mechanisms related to the MXR system.

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“…Among the arsenal of enzymes involved in animal cell detoxification, some members of the ATP Binding Cassette (ABC) superfamily are found (Bard, 2000;Rioult et al, 2014). ABC transporters were first described for their role in multidrug resistance (MDR) to chemotherapeutic drugs (Gottesman and Ling, 2006;Nielsen and Skovsgaard, 1992;Sharom, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…The MXR phenotype is supposedly ubiquitous in aquatic invertebrates. Functional and molecular assays allowed the identification and characterization of some ABC transporters members in several species including sponges (Kurelec et al, 1992), innkeeper worms (Toomey and Epel, 1993), molluscs (Faria et al, 2011;McFadzen et al, 2000;Navarro et al, 2012;Rioult et al, 2014) and crabs (Köhler et al, 1998;Minier et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Multixenobiotic resistance in Mytilus edulis : Molecular and functional characterization of an ABCG2- type transporter in hemocytes and gills

et al. 2018

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Among the cellular protection arsenal, ABC transporters play an important role in xenobiotic efflux in marine organisms. Two pumps belonging to B and C subfamily has been identified in Mytilus edulis. In this study, we investigated the presence of the third major subtype ABCG2/BCRP protein in mussel tissues. Transcript was expressed in hemocytes and with higher level in gills. Molecular characterization revealed that mussel ABCG2 transporter shares the sequence and organizational structure with mammalian and molluscan orthologs. Overall identity of the predicted amino acid sequence with corresponding homologs from other organisms was between 49% and 98%. Moreover, protein efflux activity was demonstrated using a combination of fluorescent allocrites and specific inhibitors. The accumulation of bodipy prazosin and pheophorbide A was heterogeneous in gills and hemocytes. Most of the used blockers enhanced probe accumulation at different levels, most significantly for bodipy prazosin. Moreover, Mrp classical blocker MK571 showed a polyspecificity. In conclusion, our data demonstrate that several ABC transporters contribute to MXR phenotype in the blue mussel including ABCG2 that forms an active pump in hemocytes and gills. Efforts are needed to distinguish between the different members and to explore their single function and specificity towards allocrites and chemosensitizers.

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The multi-xenobiotic resistance (MXR) efflux activity in hemocytes of Mytilus edulis is mediated by an ATP binding cassette transporter of class C (ABCC) principally inducible in eosinophilic granulocytes

Cited by 20 publications

References 61 publications

Assessment of sperm quality in palaemonid prawns using Comet assay: methodological optimization

Assessment of sperm quality in palaemonid prawns using Comet assay: methodological optimization

Neonicotinoid insecticides are potential substrates of the multixenobiotic resistance (MXR) mechanism in the non-target invertebrate, Dreissena sp.

Multixenobiotic resistance in Mytilus edulis : Molecular and functional characterization of an ABCG2- type transporter in hemocytes and gills

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