The multifaceted nature of HIV latency

A highly multiplexed droplet digital PCR assay to measure the intact HIV-1 proviral reservoir

“…There are few publications about the presence of replicationcompetent proviruses in tissues [47][48][49][50] and none comparing intact to defective proviruses in the mucosa. Thus, it remains unknown whether mucosal sites, 51 especially the gut, [52][53][54][55] are clinically relevant reservoirs that cause intermittent plasma viremia during suppressive ART and/or viral rebound after ART cessation. 56,57 Therefore, optimizing our protocol for use with tissue specimens was a crucial step to expand future reservoir studies beyond peripheral blood.…”

SARS-CoV-2 Portrayed Against HIV: Contrary Viral Strategies in Similar Disguise

“…HIV-1 achieves productive infection by preferential integration of its viral genome in intron regions of highly expressed host genes [116,119]. The chronicity of HIV-1 is caused by latent infection of long-lived memory CD4 + T cells and constitutes a major barrier towards a cure of HIV-1 infection [120]. Latent infection is accomplished by integrating HIV-1 into transcriptionally silent regions of the genome of quiescent CD4 + T cells [121,122].…”

SARS-CoV-2 Portrayed against HIV: Contrary Viral Strategies in Similar Disguise

“…HIV-1 achieves productive infection by preferential integration of its viral genome in intron regions of highly expressed host genes [ 147 , 150 ]. The chronicity of HIV-1 is caused by latent infection of long-lived memory CD4 + T cells and constitutes a major barrier towards a cure of HIV-1 infection [ 151 ]. Latent infection is accomplished by integrating HIV-1 into transcriptionally silent regions of the genome of quiescent CD4 + T cells [ 152 , 153 ].…”