The Multifaceted Nature of Nucleobindin-2 in Carcinogenesis

Skorupska, Anna; Lenda, Rafał; Ożyhar, Andrzej; Bystranowska, Dominika

doi:10.3390/ijms22115687

Cited by 10 publications

(6 citation statements)

References 101 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In recent years, the potential link between NUCB2/ nesfatin-1 and tumorigenesis has been progressively revealed [11]. NUCB2/nesfatin-1 contributes to the development and progression of various cancers, such as prostate, colon, and breast cancers [30][31][32], and the underlying mechanism may be related to its ability to promote cell proliferation, migration, and invasion [33].…”

Section: Discussionmentioning

confidence: 99%

“…NUCB2/nesfatin-1 has been reported to promote cell proliferation, migration, and invasion in various cancers, including breast, colon, prostate, endometrial, thyroid, and bladder cancers [10]. Moreover, high expression of NUCB2/nesfatin-1 is associated with key traits of cancer as well as poor prognoses and outcomes [11]. Mechanistically, it has been shown that NUCB2/nesfatin-1 improved the migration, invasion, and EMT characteristics of colon and renal cancer cells through stimulation of the AMPK/TOC1/ZEB1 pathway [12,13].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Inhibition of NUCB2 suppresses the proliferation, migration, and invasion of rheumatoid arthritis synovial fibroblasts from patients with rheumatoid arthritis in vitro

Zhang

et al. 2022

J Orthop Surg Res

View full text Add to dashboard Cite

Rheumatoid arthritis (RA) is an autoimmune polyarthritis in which synovial fibroblasts (SF) play a major role in cartilage and bone destruction through tumorlike proliferation, migration, and invasion. Nesfatin-1, an 82-amino-acid-long peptide discovered by Oh-I in 2006, is derived from the precursor protein nucleobindin-2 (NUCB2). NUCB2/nesfatin-1 promotes cell proliferation, migration, and invasion in various tumors. We have previously shown that increased nesfatin-1 levels in the synovium may be associated with disease severity in patients with RA. However, the effect of NUCB2 on the tumorlike transformation of RASF has not yet been reported. The expression of NUCB2 mRNA in the synovium of RA and non-RA patients was further confirmed using three individual datasets from the NCBI GEO database. Gene set enrichment analysis (GSEA) was employed to explore the association between NUCB2 mRNA and RA-related gene signatures or signaling pathways in the GSE77298 dataset. Cell proliferation, migration, and invasion abilities were determined using Cell Counting Kit-8 (CCK-8), 5-ethynyl-2'-deoxyuridine (EdU), wound healing, and transwell assays, respectively. The results showed that the levels of NUCB2 mRNA in the synovium were significantly elevated in patients with RA. Moreover, GSEA showed that high expression of NUCB2 mRNA was related to gene signatures, including those involved in the cell cycle, DNA replication, extracellular matrix–receptor interaction, and focal adhesion. Furthermore, the results of CCK-8 and EdU assays indicated that inhibition of NUCB2 markedly repressed RASF proliferation. Additionally, the results of wound healing and transwell assays demonstrated that inhibition of NUCB2 significantly suppressed the migratory and invasive abilities of RASFs. Our findings are the first to demonstrate that the inhibition of NUCB2 suppresses the proliferation, migration, and invasion of RASFs in vitro.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Inhibition of NUCB2 suppresses the proliferation, migration, and invasion of rheumatoid arthritis synovial fibroblasts from patients with rheumatoid arthritis in vitro

Zhang

et al. 2022

J Orthop Surg Res

View full text Add to dashboard Cite

show abstract

“…Reinforcement 7 was applied throughout the analysis. The use of a higher reinforcements (e.g., [16][17][18], would allow the identification and quantification of nesfatin-1 at concentrations that would allow its determination directly in human fluids, e.g. plasma, serum, liquid cerebrospinal.…”

Section: Calculation Of Relative Standard Deviation Values (Rsd) and ...mentioning

confidence: 99%

“…Nucleobindin-2/Nesfatin-1 is also a component of various secreted body fluids, including saliva, synovial fluid, milk, and plasma or serum [15,16]. Therefore, there is a nowadays need to define new prognostic and predictive markers to make treatment options more personalized [6,17].…”

Section: Introductionmentioning

confidence: 99%

Application of solid-phase extraction (SPE) and high-performance liquid chromatography (HPLC) and comparison of both detection techniques (DAD and FLD) to analyse nesfatin-1 in fetal bovine serum samples

Tuzimski,

Szubartowski

2023

AChrom

View full text Add to dashboard Cite

In this study, we propose a simple, cost-effective, and sensitive high-performance liquid chromatography with both detection techniques such as diode-array detection and fluorescence detection (HPLC-DAD-FLD) for the determination of nesfatin-1 in fetal bovine serum samples. The limit of detection (LOD) and limit of quantification (LOQ) for nesfatin-1 were set at satisfactory values in the range 0.22–0.35 mg mL−1 and in the range 0.67–1.05 mg mL−1, respectively (at two different wavelengths (DAD) and at four different wavelengths (FLD)). Analyte concentrations were determined as the average value from fetal bovine serum matrix samples. The preliminary results show that the SPE procedure on Isolute Si-TsOH (SCX-3) could be used for further nesfatin-1 analyses in human serum samples. Both the SPE technique, chromatographic analysis with gradient elution mode and detection technique are fast and convenient.

show abstract

“…Nucleobindin 2 (NUCB2) is a precursor protein of nesfatin-1, which was ordinally identi ed in hypothalamic nuclei and associated with food intake and energy homeostasis [7,8]. The protein has characteristic functional domains such as a Leu/Ile rich region, two Ca 2+ binding EF-hand domains separated by an acidic amino acid-rich region, and a leucine zipper [9]. The role of NUCB2 in tumor development and metastasis is Janus-like.…”

Section: Introductionmentioning

confidence: 99%

Nucleobindin 2 inhibits senescence in gastric carcinoma

Ishibashi,

Itoh,

Oguri

et al. 2023

Preprint

View full text Add to dashboard Cite

Background Nucleobindin 2 (NUCB2) is a multifunctional protein that is associated with a variety of biological processes. Here, we focused on the functional role of NUCB2 on progression of gastric carcinoma (GC). Methods NUCB2 expression was investigated in 150 GC cases by immunohistochemistry (IHC), as well as in situ hybridization for detection of the mRNA in 10 cases. GC cell lines were used to determine whether NUCB2 expression was associated with specific cellular phenotypes. Results In GC clinical samples, NUCB2 expression was transcriptionally upregulated when compared to the non-tumoral lesions. High NUCB2 expression was significantly associated with several aggressive clinicopathological factors including deep tumor invasion, lymphovascular invasion, lymph node metastasis, and advanced clinical stages, and was a significant independent prognostic factor for predicting unfavorable progression-free survival in GC patients. In cell lines, NUCB2 knockout inhibited proliferation, susceptibility to apoptosis, and migration capability through an induction of cellular senescence; this was consistent with the significantly higher proliferation and apoptotic indices in NUCB2 IHC-high category as compared to NUCB2 IHC-low GC cases. Conclusions NUCB2-dependent inhibition of senescence in GC leads to aggressive tumor behavior by modulating proliferation, apoptosis, and migration.

show abstract

The Multifaceted Nature of Nucleobindin-2 in Carcinogenesis

Cited by 10 publications

References 101 publications

Inhibition of NUCB2 suppresses the proliferation, migration, and invasion of rheumatoid arthritis synovial fibroblasts from patients with rheumatoid arthritis in vitro

Inhibition of NUCB2 suppresses the proliferation, migration, and invasion of rheumatoid arthritis synovial fibroblasts from patients with rheumatoid arthritis in vitro

Application of solid-phase extraction (SPE) and high-performance liquid chromatography (HPLC) and comparison of both detection techniques (DAD and FLD) to analyse nesfatin-1 in fetal bovine serum samples

Nucleobindin 2 inhibits senescence in gastric carcinoma

Contact Info

Product

Resources

About