1989
DOI: 10.1002/hep.1840090221
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The multiple-indicator dilution technique for characterization of normal and retrograde flow in once-through rat liver perfusions†

Abstract: The technique of normal and retrograde rat liver perfusion has been widely used to probe zonal differences in drug-metabolizing activities. The validity of this approach mandates the same tissue spaces being accessed by substrates during both normal and retrograde perfusions. Using the multiple-indicator dilution technique, we presently examine the extent to which retrograde perfusion alters the spaces accessible to noneliminated references. A bolus dose of 51Cr-labeled red blood cells, 125I-albumin, 14C-sucro… Show more

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Cited by 36 publications
(31 citation statements)
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“…Due to the reported changes to hepatic architecture in MI (Francis & Warrell, 1993;Garnham, 1966), we used the multiple indicator technique (St-Pierre et al, 1989) to determine cellular volume of the livers in the single-pass perfusions. We found that the cellular volume of the liver comprised 49% (controls) and 53% (MI) of liver wet weight.…”
Section: Discussionmentioning
confidence: 99%
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“…Due to the reported changes to hepatic architecture in MI (Francis & Warrell, 1993;Garnham, 1966), we used the multiple indicator technique (St-Pierre et al, 1989) to determine cellular volume of the livers in the single-pass perfusions. We found that the cellular volume of the liver comprised 49% (controls) and 53% (MI) of liver wet weight.…”
Section: Discussionmentioning
confidence: 99%
“…The mean transit times of the tracers through the liver was calculated (Equations 4 ± 8; Appendix) and corrected for the mean transit time through the system without a liver (14.6+1.6 s; n=8). Cellular water space (functional volume) was determined from the dierence between total water space (obtained from [ 3 H]-H 2 O mean transit time) and the sinusoidal plus Disse volume (obtained from 14 C-sucrose mean transit time) (St-Pierre et al, 1989). At the completion of each perfusion, the livers werē ushed with chilled 0.9% sodium chloride solution, dried between absorbent towels and weighed.…”
mentioning
confidence: 99%
“…The data, when compared with previous prograde data of Schwab et al (2001), revealed that the values for total water volumes (sum of both cellular and extracellular water spaces) were greater than unity (Table 4). The apparently high value is due to the distention of the vasculature during retrograde flow, as described by St-Pierre et al (1989) and Xu et al (1990). The MTTs for labeled red blood cells, albumin, and sucrose were all increased with retrograde flow, especially for data of the first injection, although changes in the second injection were attenuated.…”
Section: Resultsmentioning
confidence: 81%
“…In situ single-pass liver perfusion under retrograde flow was carried out as previously described (St-Pierre et al, 1989) with perfusate (12 ml/min) at 37°C entering via the hepatic vein and exiting via the portal vein; the hepatic artery was ligated. Perfusate consisted of bovine erythrocytes (20%), freshly obtained and washed (kind gift of Ryding-Regency Meat Packers, Toronto, ON, Canada), 5% bovine serum albumin, and 17 mM glucose (Baxter Travenol Laboratories, Deerpark, IL) in Krebs-Henseleit bicarbonate solution (pH 7.4).…”
Section: Rat Liver Perfusionmentioning
confidence: 99%
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