The multiple interactions between growth factors and microenvironment in vivo

Bo, Jin’gen; Yang, Jian; Zhu, Dunwan; Li, Junjie; Yao, Kangde

doi:10.1007/s11434-006-0761-9

Cited by 3 publications

(2 citation statements)

References 55 publications

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“…The proliferation of osteoblast is related to the growth factors and intracellular calcium [3,14] . The light can affect the intracellular calcium [15] , and the proliferation of osteoblasts stimulated by EMF is also related to the growth factors, intra-and extra-cellular calcium [6][7][8] , but whether the communication between stimulated and un-stimulated cells is related to calcium and the growth factors is still obscure for lack of evidence.…”

Section: Discussionmentioning

confidence: 99%

Communication between osteoblasts stimulated by electromagnetic fields

Zhang

2007

CHINESE SCI BULL

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Pulsed electromagnetic field can affect the proliferation of osteoblasts, but the mechanism is obscure yet. The communication between osteoblasts, isolated from calvaria bone of newborn SD rats and stimulated with the rectangular electromagnetic field of 15 Hz and 4 mT, was studied. Our results showed that the osteoblasts radiated a kind of light after they were stimulated with the electromagnetic field and it is the light that promotes the proliferation of un-stimulated osteoblasts. electromagnetic field, osteoblast, proliferation, light, communication Pulsed electromagnetic fields (PEMF) are effective for patients with delayed fracture healing and non-unions [1][2][3] and there are many reports about the effects of PEMF on osteoblasts [4][5][6][7][8][9] . The effects of PEMF on the proliferation of osteoblasts are related to the intracellular Calcium pathway [6 -8] and DNA transcription [9] . The frequency and intensity of PEMF were also dominant to its biological effects. The mechanism is obscure yet although the biological effects of PEMF on osteoblasts have been studied for many decades. A funny and important phenomenon, which was concerned with the communication between osteoblasts after they were treated with PEMF, was demonstrated in this paper.Stimulating unit composed of the waveform generator, amplifier and coils, which can provide a uniform electromagnetic field (EMF) for treated cells, was developed. The waveform generator was an extremely low frequency function generator that can generate the potential signals with sinusoidal, triangular and rectangular waveforms. After being amplified, the signals were output to the coils. The coils formed a cylindrical solenoid (25 cm long and 5 cm in inner diameter) which was consisted of 1000 turns of copper wire (diameter 1 mm) surrounding on a plastic tube. EMF in the solenoid is approximately uniform. The cells contained in a chamber can be placed in the middle of the solenoid with a plastic shelf and the magnetic flux is vertical to the chamber. The intensity of EMF in the solenoid was computed with the formula B = μ 0 nI (n = 4000, μ 0 = 4π×10 −7 H/m) and the current I was measured with A622 current probe (Tektronix, USA). The frequency, intensity and duty cycle of the rectangular electromagnetic field used in the study are respectively 15 Hz, 4 mT and 15%.

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Section: Discussionmentioning

confidence: 99%

Communication between osteoblasts stimulated by electromagnetic fields

Zhang

2007

CHINESE SCI BULL

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“…Angiogenesis is a multi-step dynamic process involving complex interactions between vascular cells and the corresponding extracellular environment [2–4]. During this process, numerous pro-angiogenic factors, such as fibroblast growth factor (FGF), vascular endothelial growth factor (VEGF), angiopoietin-1, and stromal-derived factor 1, are secreted from new blood vessel formation sites.…”

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confidence: 99%

A novel in vitro angiogenesis model based on a microfluidic device

Dai

Cai

et al. 2011

Chin. Sci. Bull.

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Angiogenesis is very important for many physiological and pathological processes. However, the molecular mechanisms of angiogenesis are unclear. To elucidate the molecular mechanisms of angiogenesis and to develop treatments for “angiogenesis- dependent” diseases, it is essential to establish a suitable in vitro angiogenesis model. In this study, we created a novel in vitro angiogenesis model based on a microfluidic device. Our model provides an in vivo-like microenvironment for endothelial cells (ECs) cultures and monitors the response of ECs to changes in their microenvironment in real time. To evaluate the potential of this microfluidic device for researching angiogenesis, the effects of pro-angiogenic factors on ECs proliferation, migration and tube-like structure formation were investigated. Our results showed the proliferation rate of ECs in 3D matrix was significantly promoted by the pro-angiogenic factors (with an increase of 59.12%). With the stimulation of pro-angiogenic factors gradients, ECs directionally migrated into the Matrigel from low concentrations to high concentrations and consequently formed multi-cell chords and tube-like structures. These results suggest that the device can provide a suitable platform for elucidating the mechanisms of angiogenesis and for screening pro-angiogenic or anti-angiogenic drugs for “angiogenesis-dependent” diseases.

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Fibrin Glues in Combination with Mesenchymal Stem Cells to Develop a Tissue-Engineered Cartilage Substitute

Ahmed

Giulivi²,

Griffith³

et al. 2011

Tissue Engineering Part A

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Damage of cartilage due to traumatic or pathological conditions results in disability and severe pain. Regenerative medicine, using tissue engineering-based constructs to enhance cartilage repair by mobilizing chondrogenic cells, is a promising approach for restoration of structure and function. Fresh fibrin (FG) and platelet-rich fibrin (PR-FG) glues produced by the CryoSeal(®) FS System, in combination with human bone marrow-derived mesenchymal stem cells (BM-hMSCs), were evaluated in this study. We additionally tested the incorporation of heparin-based delivery system (HBDS) into these scaffolds to immobilize endogenous growth factors as well as exogenous transforming growth factor-β(2). Strongly, CD90+ and CD105+ hMSCs were encapsulated into FG and PR-FG with and without HBDS. Encapsulation of hMSCs in PR-FG led to increased expression of collagen II gene at 2.5 weeks; however, no difference was observed between FG and PR-FG at 5 weeks. The incorporation of HBDS prevented the enhancement of collagen II gene expression. BM-hMSCs in FG initially displayed enhanced aggrecan gene expression and increased accumulation of Alcian blue-positive extracellular matrix; incorporation of HBDS into these glues did not improve aggrecan gene expression and extracellular matrix accumulation. The most significant effect on cartilage marker gene expression and accumulation was observed after encapsulation of hMSCs in FG. We conclude that FG is more promising than PR-FG as a scaffold for chondrogenic differentiation of hMSCs; however, immobilization of growth factors inside these fibrin scaffolds with the HBDS system has a negative impact on this process. In addition, BM-hMSCs are valid and potentially superior alternatives to chondrocytes for tissue engineering of articular cartilage.

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The multiple interactions between growth factors and microenvironment in vivo

Cited by 3 publications

References 55 publications

Communication between osteoblasts stimulated by electromagnetic fields

Communication between osteoblasts stimulated by electromagnetic fields

A novel in vitro angiogenesis model based on a microfluidic device

Fibrin Glues in Combination with Mesenchymal Stem Cells to Develop a Tissue-Engineered Cartilage Substitute

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