The multiplication of transmissible gastroenteritis viruses in several cell lines originated from porcine kidney and effects of trypsin on the growth of the viruses.

Abstract

Plaque formation, replication and related cytopathic function of 9 strains of transmissible gastroenteritis (TGE) virus were examined in primary cells and cell lines such as CPK, IB-RS-2, ESK, and PK-15 originated from porcine kidney and the effects of trypsin on the replication of TGE virus were examined in CPK cells. All strains produced a cytopathic effect and grew well in CPK cells as well as in primary porcine kidney cells. The effect of trypsin on the plaque formation was different from strains. The number of plaques produced by strains TO-163, Ukiha and Niigata increased from 2.6 to 3.52 times when trypsin was present in the medium during incubation at 37 degrees C for 1 hr after adsorption of the virus at 4 degrees C for 2 hr. The plaque sizes of TO-163, h-5, Ukiha and Niigata became larger from 1.4 to 1.7 times, when trypsin was present in the agar MEM overlay.

Sequence Comparison of the ORF 7 Region of Transmissible Gastroenteritis Viruses Isolated in Japan

“…These nine strains were isolated in Japan from 1956 to 1997 [6]. American strains of Miller and Purdue were used as reference strains [9].…”

Development of Reverse Transcriptase PCR and Nested PCR to Detect Porcine Hemagglutinating Encephalomyelitis Virus

“…Viruses: HEV 67N strain (18) and ONS204 strain (6), coronaviruses of bovine coronavirus (BCV) Mebus strain [17], mouse hepatitis virus (MHV) JHM strain [16], transmissible gastroenteritis virus (TGEV) h5 strain [12], and the arterivirus porcine reproductive and respiratory syndrome virus (PRRSV) EDRD strain [19] were used.…”

“…DBT cell was used for the preparation of MHV [16]. CPK cell was used for the preparation of TGEV [12]. MARC145 cell was used for the preparation of PRRSV [19].…”

Reverse transcription-loop-mediated isothermal amplification for the detection of rodent coronaviruses

“…Porcine hemagglutinating encephalomyelitis virus strain 67N (HEV-67N), HEV-NT9, and HEV-VW3 were grown in SK-K cells cultured in EMEM . Porcine epidemic diarrhea virus strain 3 (PEDV-3) and porcine transmissible gastroenteritis virus strain TO (TGEV-TO) were grown in Vero and CPK cells, respectively, cultured in EMEM (Hofmann and Wyler, 1988;Honda et al, 1990). Two strains of bovine coronavirus (BCV-Kakegawa and BCV-Nebraska) were grown in BEK-1 cells cultured in EMEM containing 10% (v/v) tryptose phosphate broth (Inaba et al, 1976).…”