2013
DOI: 10.1523/jneurosci.5128-12.2013
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The Munc13 Proteins Differentially Regulate Readily Releasable Pool Dynamics and Calcium-Dependent Recovery at a Central Synapse

Abstract: The Munc13 gene family encodes molecules located at the synaptic active zone that regulate the reliability of synapses to encode information over a wide range of frequencies in response to action potentials. In the CNS, proteins of the Munc13 family are critical in regulating neurotransmitter release and synaptic plasticity. Although Munc13-1 is essential for synaptic transmission, it is paradoxical that Munc13-2 and Munc13-3 are functionally dispensable at some synapses, although their loss in other synapses … Show more

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Cited by 97 publications
(124 citation statements)
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“…Quantal parameters were estimated from GC-mediated uEPSCs recorded at different extracellular calcium concentrations ([Ca 2ϩ ] o : 1, 2, 5, or 10 mM, Ն80 repetitions at each concentration, 5 s stimulus interval) by a multiple-probability fluctuation analysis (MPFA) well suited for unitary connections (Clements and Silver, 2000). In the MPFA, the variance of charges (corrected for the variance of visually identified failures) is plotted versus the mean charge (including failures), which allows extracting the quantal size q, p r , and the binomial parameter N from a parabolic fit to the data (for details, see Hallermann et al, 2010).…”
Section: Methodsmentioning
confidence: 99%
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“…Quantal parameters were estimated from GC-mediated uEPSCs recorded at different extracellular calcium concentrations ([Ca 2ϩ ] o : 1, 2, 5, or 10 mM, Ն80 repetitions at each concentration, 5 s stimulus interval) by a multiple-probability fluctuation analysis (MPFA) well suited for unitary connections (Clements and Silver, 2000). In the MPFA, the variance of charges (corrected for the variance of visually identified failures) is plotted versus the mean charge (including failures), which allows extracting the quantal size q, p r , and the binomial parameter N from a parabolic fit to the data (for details, see Hallermann et al, 2010).…”
Section: Methodsmentioning
confidence: 99%
“…Identical parameters were applied to analyze both genotypes. The ratio of Munc13-XFP/bassoon signal intensity (Chen et al, 2013) was calculated for each pixel within the delineated active zone for creating ratio images (see Fig. 4 A, B) or averaged per active zone for statistical analysis (see Fig.…”
Section: Methodsmentioning
confidence: 99%
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“…On the other hand, Munc13-4 is involved in regulated exocytosis in hematopoietic secretory cells, such as cytotoxic T lymphocytes (CTLs), platelets, neutrophils, and mast cells (7,12,27,30,33,35). It is also known that in neurons, chromaffin cells, and CTLs, multiple Munc13 isoforms are endogenously expressed, and play some redundant roles in the promotion of the docking and priming of secretory vesicles/granules (8,10,24,36). In mast cells, Munc13-4 is shown to be crucial for degranulation (11,35,38); however, the involvement of other Munc13 isoform(s) still remains unknown.…”
Section: Rt-pcr and Quantitative Real-time Rt-pcrmentioning
confidence: 99%