The murine heat‐stable antigen: a differentiation antigen expressed in both the hematolymplioid and neural cell lineages

Rougon, Geneviève; Alterman, Lesley A.; Dennis, K. Neeld; Guo, Xiajun; Kinnon, Christine

doi:10.1002/eji.1830210611

Cited by 64 publications

(56 citation statements)

References 31 publications

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“…Sections were processed as described previously (C alaora et al, 1996). Briefly, they were incubated overnight at 4°C with one of the following antibodies: anti-mCD24, rat monoclonal IgG (dilution 1:100, prepared in Rougon's laboratory; Rougon et al, 1991), anti-polysialic acid (PSA)-neural cell adhesion molecule (NCAM), mouse monoclonal IgM (dilution 1:100, prepared in Rougon's laboratory; Rougon et al, 1986), anti-GFAP, rabbit polyclonal IgG (1:100; Sigma, St. Quentin Fallavier, France), or monoclonal mouse IgG (1:2000; Sigma) and anti-S100, rabbit polyclonal IgG (1:100; Dako, Glostrup, Denmark). Sections were then washed in PBS before incubation in the corresponding fluorescent secondary antibodies (1:50; Immunotech S.A., Marseille, France) for 1 hr at room temperature.…”

Section: Methodsmentioning

confidence: 99%

“…mCD24 (mouse Cluster of Differentiation 24) is a small protein of 30 amino acids exhibiting several highly glycosylated tissue-specific isoforms that was originally described as a pre-B lymphocyte marker (Kay et al, 1991;Wenger et al, 1991). mCD24 was also described in other cell types such as T-cells (Crispe and Bevan, 1987), regenerating muscle cells (Figarella-Branger et al, 1993), or neurons (Rougon et al, 1991;Kadmon et al, 1992). Among the three isolated genes susceptible to encode mCD24, only one has been shown to be expressed (Wenger et al, 1991).…”

Section: Abstract: Mcd24; Proliferation; Adult Neurogenesis; Neural mentioning

confidence: 99%

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Increased Neurogenesis in Adult mCD24-Deficient Mice

2002

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mCD24, a glycosylphosphatidylinositol-anchored highly glycosylated molecule, is expressed on differentiating neurons during development. In the adult CNS, its expression is restricted to immature neurons located in two regions showing ongoing neurogenesis: the subventricular zone (SVZ) of the lateral ventricle pathway and the dentate gyrus (DG) of the hippocampal formation. Here, combining bromodeoxyuridine (BrdU) and proliferating cell nuclear antigen labelings we confirmed that mCD24 is expressed on proliferating cells. To determine whether the inactivation of the molecule may affect adult neurogenesis, we analyzed the phenotype of mCD24-deficient mice (mCD24Ϫ/Ϫ). We labeled cells in S-phase with a pulse, a long, or a cumulative administration of BrdU and analyzed cells in different zones according to their dividing rate (rapid and slow) both in the control and mCD24Ϫ/Ϫ. We found a significant increase in the number of rapid (in the SVZ and the DG) and slow (in the SVZ) proliferating cells. Cumulative assays revealed a global reduction of the total cell cycle duration of rapidly proliferating precursors of SVZ. We investigated the fate of supernumerary cells and observed an increased number of apoptotic cells (terminal deoxynucleotidyl transferasemediated biotinylated UTP nick end labeling) in the mutant SVZ. Furthermore, we found no difference in the size of the olfactory bulb between wild-type (WT) and mutant mice. In support, mCD24 deletion did not appear to affect migration in the migratory stream. A comparison of the organization of migrating precursors between WT and mCD24 Ϫ/Ϫ, both in vivo at the optic and electron microscopic levels and in SVZ cultured explants, did not show any changes in the arrangement of neuroblasts in chain-like structures.Altogether, our data suggest that mCD24 regulates negatively cell proliferation in zones of secondary neurogenesis.

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Section: Methodsmentioning

confidence: 99%

Section: Abstract: Mcd24; Proliferation; Adult Neurogenesis; Neural mentioning

confidence: 99%

Increased Neurogenesis in Adult mCD24-Deficient Mice

2002

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“…The human CD24 molecule has additional serine and threonine residues, rendering the molecule like a mucin. 3 CD24 protein isolated from different tissues or cell types has different molecular weights, ranging from 20 to 70 kd, 3,[8][9][10] demonstrating that the glycosylation of heat-stable antigen and CD24 is highly variable and cell-type dependent. The saccharide structure of CD24 from mouse brain is now being elucidated.…”

Section: Introductionmentioning

confidence: 99%

CD24: from A to Z

et al. 2010

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As a testament to the importance of CD24, researchers with diverse interests, including adaptive immunity, inflammation, autoimmune diseases and cancer, have encountered CD24. CD24 is overexpressed in many cancers and appears oncogenic. In the adaptive immune response, CD24 is a redundant costimulatory molecule in costimulation-rich lymphoid organs but is essential in selected target organs tested, such as brain and skin. More recent studies suggest it may have a role in discriminating danger and pathogen-associated molecular patterns by dendritic cells. The biology of CD24 is intriguing but poorly understood. Here we summarize the major findings associated with CD24 to stimulate new ideas for further research that may reveal the underlying link among the diverse processes mediated by CD24.

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“…Recent studies from several groups revealed that HSA plays a major role in regulating interaction between T and B lymphocytes (12,19,28,29), T-cell clonal expansion (10,18,29), and induction of immunological memory (30,41). The expression of HSA is largely restricted to cells of hematopoietic (1,5,34,36) and neuronal (19,36) origins. Among the hematopoietic cells, HSA is expressed on a variety of cell types, including erythrocytes (39), thymocytes (5), B cells (5,19,29,36), macrophages (8), and Langerhans cells (10).…”

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confidence: 99%

Regulation of the Stability of Heat-Stable Antigen mRNA by Interplay between Two Novel cis Elements in the 3′ Untranslated Region

Zhou

Guo

Yang

1998

Molecular and Cellular Biology

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The heat-stable antigen (HSA) is a costimulatory molecule for T-cell activation. Its expression is strictly regulated during lymphocyte development and differentiation. Recent studies using HSA-transgenic mice have demonstrated that this regulated expression is critical for normal development of T and B lymphocytes. However, the mechanisms that control the expression of HSA are largely unknown. HSA mRNA is comprised of a 0.23-kb open reading frame and a 1.5-kb 3 untranslated region (3UTR). The function of the long 3UTR has not been addressed. Here we investigate the role of the 3UTR of HSA mRNA. We show that a 160-bp element, located in the region of nucleotides 1465 to 1625 in the 3UTR of HSA mRNA, promotes RNA degradation and that this effect is neutralized by a 43-bp fragment approximately 1 kb upstream of the negative cis element. Both positive and negative cis elements in the HSA mRNA are distinct from other sequences that are known to modulate mRNA stability. These results provide direct evidence that the interplay between two novel cis elements in the 3UTR of HSA mRNA determines cell surface HSA expression by modulating its RNA stability.

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The murine heat‐stable antigen: a differentiation antigen expressed in both the hematolymplioid and neural cell lineages

Cited by 64 publications

References 31 publications

Increased Neurogenesis in Adult mCD24-Deficient Mice

Increased Neurogenesis in Adult mCD24-Deficient Mice

CD24: from A to Z

Regulation of the Stability of Heat-Stable Antigen mRNA by Interplay between Two Novel cis Elements in the 3′ Untranslated Region

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