The mutagenic potency of 1,8-dinitropyrene in cultured mouse lymphoma cells

The genotoxicity, metabolism and carcinogenicity of nitrated polycyclic aromatic hydrocarbons

“…Moreover, in some cell lines (ejj., mouse lymphoma) prolonged exposure is required for expression of the mutagenicity (136,137). This trend is reversed in human HepG2 cells (but not in other human cells) in which the highly mutagenic (in Salmonella) dinitropyrenes are inactive and 1-nitropyrene is active (119,149).…”

Short-Term Bioassays in the Analysis of Complex Environmental Mixtures III

“…On the other hand, only the standard incubation period (3 h) was required for the demonstration of the mutagenicity of 2-nitrofluorene, 4-nitroquinoline-l-oxide, and nitrofurans in these cells (Amacher et al, 1979;Cole et al, 1982); thus implying that the nitroreductases recognizing nitrofluorene, 4-nitroquinoline-l-oxide, and nitrofurans were present constitutively and did not require induction.…”

“…This provided the rationale for the prolonged incubation of mammalian cells and the subsequent demonstration of the mutagenicity of 1,8-dinitropyrene in mouse lymphoma cells, when incubation was for 48 h (Cole et al, 1982). On the other hand, only the standard incubation period (3 h) was required for the demonstration of the mutagenicity of 2-nitrofluorene, 4-nitroquinoline-l-oxide, and nitrofurans in these cells (Amacher et al, 1979;Cole et al, 1982); thus implying that the nitroreductases recognizing nitrofluorene, 4-nitroquinoline-l-oxide, and nitrofurans were present constitutively and did not require induction.…”

Nitropyrene: DNA Binding and Adduct Formation in Respiratory Tissues

“…Nitro-substituted PAHs (NO2-PAH) have been found in extracts from diesel particles (2)(3)(4)(5) and ambient air particulates (6). Many of these NO2-PAH are mutagenic in mammalian cells (7)(8)(9)(10) as well as in bacteria (8)(9)(10)(11)(12) and may, therefore, be a potential risk to human health.…”