The mvp2 mutation affects the generative transition through the modification of transcriptome pattern, salicylic acid and cytokinin metabolism in Triticum monococcum

Boldizsár, Ákos; Vaňková, Radomı́ra; Novák, Alíz; Kalapos, Balázs; Gulyás, Zsolt; Pál, Magda; Floková, Kristýna; Janda, Tibor; Galiba, Gábor; Kocsy, Gábor

doi:10.1016/j.jplph.2016.07.005

Cited by 6 publications

(4 citation statements)

References 55 publications

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“…Several components of the low temperature-induced CBF pathway have already been revealed, primarily in Arabidopsis (Chinnusamy et al 2003; Agarwal et al 2006; Badawi et al 2008; Boldizsár et al 2016). To collect more details about the regulation of CBF14 gene expression by low temperature and light, we tested transcriptional responses of genes, which act in the low temperature pathway upstream of CBF14 in Arabidopsis and possess homologs in wheat and/or barley.…”

Section: Resultsmentioning

confidence: 99%

“…Synthesis of cDNA was done from 1 μg of total RNA using M-MLV Reverse Transcriptase (Affymetrix, Santa Clara, CA, USA) according to supplier’s protocol. The KAPA SYBR ® FAST Universal 2× qPCR Master Mix (Kapabiosystems, Wilmington, USA), gene specific and house-keeping primers ( S1 Table; (Burton et al 2004; Paolacci et al 2009; Campoli et al 2009; Dhillon et al 2010; Morran et al 2011; Boldizsár et al 2016; Novák et al 2016)) and CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA) were used for quantitative real-time PCR reactions. The relative gene expressions were calculated using the ΔΔCt method (Livak and Schmittgen 2001), where Ct values were normalized by the Ct values of house-keeping genes ( cyclophilin for barley and the Ta30797 phosphogluconate dehydrogenase for wheat) and relative to the control samples.…”

Section: Methodsmentioning

confidence: 99%

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Light and Temperature Signalling at the Level of CBF14 Gene Expression in Wheat and Barley

et al. 2017

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The wheat and barley CBF14 genes have been newly defined as key components of the light quality-dependent regulation of the freezing tolerance by the integration of phytochrome-mediated light and temperature signals. To further investigate the wavelength dependence of light-induced CBF14 expression in cereals, we carried out a detailed study using monochromatic light treatments at an inductive and a non-inductive temperature. Transcript levels of CBF14 gene in winter wheat Cheyenne, winter einkorn G3116 and winter barley Nure genotypes were monitored. We demonstrated that (1) CBF14 is most effectively induced by blue light and (2) provide evidence that this induction does not arise from light-controlled CRY gene expression. (3) We demonstrate that temperature shifts induce CBF14 transcription independent of the light conditions and that (4) the effect of temperature and light treatments are additive. Based on these data, it can be assumed that temperature and light signals are relayed to the level of CBF14 expression via separate signalling routes.Electronic supplementary materialThe online version of this article (doi:10.1007/s11105-017-1035-1) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Light and Temperature Signalling at the Level of CBF14 Gene Expression in Wheat and Barley

et al. 2017

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“…Studied genes related to amino acid metabolism: arginine decarboxylase ( ADC ), argininosuccinate lyase ( ArgSUL ), aspartate transaminase ( AspTA ), glutamate dehydrogenase ( GluDH ), nitrate reductase ( NR ), ornithine aminotransferase ( OrnATF ), pyrroline-5-carboxylate reductase ( P5CR , Pro synthesis) and serine hydroxymethyltransferase ( SerHMTF ). The values of the relative gene expression changes were calculated as described earlier applying Ta30797 gene reference, which is similar to phosphogluconate dehydrogenase protein [33].…”

Section: Methodsmentioning

confidence: 99%

Light intensity and spectrum affect metabolism of glutathione and amino acids at transcriptional level

et al. 2019

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The effects of various light intensities and spectral compositions on glutathione and amino acid metabolism were compared in wheat. Increase of light intensity (low—normal—high) was accompanied by a simultaneous increase in the shoot fresh weight, photosynthetic activity and glutathione content. These parameters were also affected by the modification of the ratios of blue, red and far-red components (referred to as blue, pink and far-red lights) compared to normal white light. The photosynthetic activity and the glutathione content decreased to 50% and the percentage of glutathione disulfide (characterising the redox state of the tissues) in the total glutathione pool doubled in far-red light. The alterations in the level and redox state of the antioxidant glutathione resulted from the effect of light on its synthesis as it could be concluded from the changes in the transcription of the related genes. Modification of the light conditions also greatly affected both the amount and the ratio of free amino acids. The total free amino acid content was greatly induced by the increase of light intensity and was greatly reduced in pink light compared to the normal intensity white light. The concentrations of most amino acids were similarly affected by the light conditions as described for the total free amino acid content but Pro, Met, Thr, ornithine and cystathionine showed unique response to light. As observed for the amino acid levels, the expression of several genes involved in their metabolism also enhanced due to increased light intensity. Interestingly, the modification of the spectrum greatly inhibited the expression of most of these genes. Correlation analysis of the investigated parameters indicates that changes in the light conditions may affect growth through the adjustment of photosynthesis and the glutathione-dependent redox state of the tissues. This process modifies the metabolism of glutathione and amino acids at transcriptional level.

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“…This process is generally called frost hardening or cold hardening. Cold hardening is the result of various physical and biochemical processes, including the adjustment of membrane composition and the accumulation of certain protective substances, among them stabilising compounds such as polyamines, osmoprotectants or antioxidants (Winfield et al, 2010 ; Boldizsár et al, 2016a , b ). The synthesis of these compounds and the regulation of acclimation mechanisms are mediated by a complex signal transduction network (Van Buskirk and Thomashow, 2006 ).…”

Section: Introductionmentioning

confidence: 99%

Janus-Faced Nature of Light in the Cold Acclimation Processes of Maize

et al. 2018

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Exposure of plants to low temperature in the light may induce photoinhibitory stress symptoms, including oxidative damage. However, it is also known that light is a critical factor for the development of frost hardiness in cold tolerant plants. In the present work the effects of light during the cold acclimation period were studied in chilling-sensitive maize plants. Before exposure to chilling temperature at 5°C, plants were cold acclimated at non-lethal temperature (15°C) under different light conditions. Although exposure to relatively high light intensities during cold acclimation caused various stress symptoms, it also enhanced the effectiveness of acclimation processes to a subsequent severe cold stress. It seems that the photoinhibition induced by low temperature is a necessary evil for cold acclimation processes in plants. Greater accumulations of soluble sugars were also detected during hardening at relatively high light intensity. Certain stress responses were light-dependent not only in the leaves, but also in the roots. The comparison of the gene expression profiles based on a microarray study demonstrated that the light intensity is at least as important a factor as the temperature during the cold acclimation period. Differentially expressed genes were mainly involved in most of assimilation and metabolic pathways, namely photosynthetic light capture via the modification of chlorophyll biosynthesis and the dark reactions, carboxylic acid metabolism, cellular amino acid, porphyrin or glutathione metabolic processes, ribosome biogenesis and translation. Results revealed complex regulation mechanisms and interactions between cold and light signalling processes.

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The mvp2 mutation affects the generative transition through the modification of transcriptome pattern, salicylic acid and cytokinin metabolism in Triticum monococcum

Cited by 6 publications

References 55 publications

Light and Temperature Signalling at the Level of CBF14 Gene Expression in Wheat and Barley

Light and Temperature Signalling at the Level of CBF14 Gene Expression in Wheat and Barley

Light intensity and spectrum affect metabolism of glutathione and amino acids at transcriptional level

Janus-Faced Nature of Light in the Cold Acclimation Processes of Maize

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