The myokine irisin: localization and effects in swine late medium and large antral ovarian follicle

Basini, Giuseppina; Bussolati, Simona; Iannarelli, Mattia; Ragionieri, Luisa; Grolli, Stefano; Ramoni, Roberto; Dodi, Alessandra; Gazza, Ferdinando; Grasselli, Francesca

doi:10.1016/j.domaniend.2020.106576

Cited by 13 publications

(13 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Irisin can promote the expression of LH in the pituitary gland of tilapia at a concentration of 1 nM, while the lowest concentration that promotes the expression of FSH is 10 nM [ 96 ]. In porcine ovarian granulosa cells, irisin at a low concentration (50 ng/ml) could promote progesterone secretion, while a high concentration (150 ng/ml) could inhibit the production of progesterone [ 103 ]. In addition, the effect of irisin is time-dependent.…”

Section: Irisin and Ovarian/testicular And Sex Hormonesmentioning

confidence: 99%

Irisin: circulating levels in serum and its relation to gonadal axis

Luo

Qiao

et al. 2022

Endocrine

View full text Add to dashboard Cite

Irisin is an exercise-induced myokine/adipokine in mice and humans that plays an important role in ‘browning’ of white adipose tissue and has shown great potential as a treatment for some metabolic diseases, such as obesity, insulin resistance, and inflammation. The circulating irisin level is reported to be associated with exercise, obesity, diet, diseases, and exposure to different pharmacological agents. Several studies have attempted to characterize the role of irisin in PCOS and other reproductive diseases, but contradictory results have been reported. Our previous study showed that irisin may serve further functions in folliculogenesis and fertility. In this review, we present the current knowledge on the physiology of irisin and its role in gonadal axis. Firstly, we describe irisin circulating levels and speculate on the potential mechanisms involved in irisin secretion and regulation. Then, we focus on the irisin levels in PCOS, and explore the relationships between, BMI, insulin resistance, and hyperandrogenism. Finally, we present the results from animal interventional studies and in vitro experiments to investigate the relationship between irisin and gonadal axis, indicating its novel effects on reproduction and fertility.

show abstract

Section: Irisin and Ovarian/testicular And Sex Hormonesmentioning

confidence: 99%

Irisin: circulating levels in serum and its relation to gonadal axis

Luo

Qiao

et al. 2022

Endocrine

View full text Add to dashboard Cite

show abstract

“…Earlier studies investigated the FNDC5/irisin expression in rat [ 64 ] and human [ 65 , 66 , 67 ] ovaries, in addition to the placenta, testis, pituitary, skeletal and cardiac muscles, brain, and brown adipose tissue [ 65 ]. A recent study by Basini et al has also provided experimental evidence of irisin presence in the pig ovary [ 68 ]. The irisin presence or local expression in the follicle of the pig ovary verified its probable regulatory role in the main functional aspect of granulosa cells [ 68 ].…”

Section: Discussionmentioning

confidence: 99%

“…A recent study by Basini et al has also provided experimental evidence of irisin presence in the pig ovary [ 68 ]. The irisin presence or local expression in the follicle of the pig ovary verified its probable regulatory role in the main functional aspect of granulosa cells [ 68 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative Genomic Characterization of Buffalo Fibronectin Type III Domain Proteins: Exploring the Novel Role of FNDC5/Irisin as a Ligand of Gonadal Receptors

Hassan

Fatima

et al. 2021

Biology

View full text Add to dashboard Cite

FN-III proteins are widely distributed in mammals and are usually involved in cellular growth, differentiation, and adhesion. The FNDC5/irisin regulates energy metabolism and is present in different tissues (liver, brain, etc.). The present study aimed to investigate the physiochemical characteristics and the evolution of FN-III proteins and FNDC5/irisin as a ligand targeting the gonadal receptors including androgen (AR), DDB1 and CUL4 associated factor 6 (DCAF6), estrogen-related receptor β (ERR-β), estrogen-related receptor γ (ERR-γ), Krüppel-like factor 15 (KLF15), and nuclear receptor subfamily 3 group C member 1 (NR3C1). Moreover, the putative role of irisin in folliculogenesis and spermatogenesis was also elucidated. We presented the molecular structure and function of 29 FN-III genes widely distributed in the buffalo genome. Phylogenetic analysis, motif, and conserved domain pattern demonstrated the evolutionary well-conserved nature of FN-III proteins with a variety of stable to unstable, hydrophobic to hydrophilic, and thermostable to thermo-unstable properties. The comparative structural configuration of FNDC5 revealed amino acid variations but still the FNDC5 structure of humans, buffalo, and cattle was quite similar to each other. For the first time, we predicted the binding scores and interface residues of FNDC5/irisin as a ligand for six representative receptors having a functional role in energy homeostasis, and a significant involvement in folliculogenesis and spermatogenesis in buffalo.

show abstract

“…We studied cell viability by means of MTT test (Sigma, St. Louis, MO, USA), which is a colorimetric assay based on tetrazolium salt 3-[4,5-dimethylthrazol-2-yl]-2,5-dipheniltetrazolium bromide (MTT) reduction by mitochondrial dehydrogenase [ 32 ]. A total of 10 4 cells were seeded in 96-well plates in 200 μL CM and treated with OXB for 48 h, as indicated above.…”

Section: Methodsmentioning

confidence: 99%

“…The amplification was performed at the same conditions as described above except for cycle number (34). Actin (ACT) was chosen as housekeeping gene co-amplified with the interest genes [ 17 , 32 , 37 ].…”

Section: Methodsmentioning

confidence: 99%

Effects of Orexin B on Swine Granulosa and Endothelial Cells

Grasselli

Bussolati

Grolli

et al. 2021

Animals

View full text Add to dashboard Cite

In addition to the well-known central modulatory role of orexins, we recently demonstrated a peripheral involvement in swine granulosa cells for orexin A and in adipose tissue for orexin B (OXB). The aim of present research was to verify immunolocalization of OXB and its potential role in modulating the main features of swine granulosa cells. In particular, we explored the effects on granulosa cell proliferation (through the incorporation of bromodeoxyuridine), cell metabolic activity (as indirect evaluation by the assessment of ATP), steroidogenic activity (by immunoenzymatic examination) and redox status (evaluating the production of superoxide anion by means of the WST test, production of nitric oxide through the use of the Griess test and the non-enzymatic reducing power by FRAP test). Our data point out that OXB does not modify granulosa cell growth, steroidogenesis and superoxide anion generation. On the contrary, the peptide stimulates (p < 0.05) nitric oxide output and non-enzymatic reducing power. Since new vessel growth is crucial for ovarian follicle development, a further aim of this study was to explore the expression of prepro-orexin and the effects of OXB on swine aortic endothelial cells. We found that the peptide is ineffective in modulating cell growth, while it inhibits redox status parameters. In addition, we demonstrated a stimulatory effect on angiogenesis evaluated in fibrin gel angiogenesis assay. Taken together, OXB appears to be potentially involved in the modulation of redox status in granulosa and endothelial cells and we could argue an involvement of the peptide in the follicular angiogenic events.

show abstract

The myokine irisin: localization and effects in swine late medium and large antral ovarian follicle

Cited by 13 publications

References 65 publications

Irisin: circulating levels in serum and its relation to gonadal axis

Irisin: circulating levels in serum and its relation to gonadal axis

Comparative Genomic Characterization of Buffalo Fibronectin Type III Domain Proteins: Exploring the Novel Role of FNDC5/Irisin as a Ligand of Gonadal Receptors

Effects of Orexin B on Swine Granulosa and Endothelial Cells

Contact Info

Product

Resources

About