ObjectivesMuscle activity during crouched walking has been previously studied in the context of the evolution of hominin bipedalism and human movement disorders. However, crouched walking could also be used in approach hunting where postural height (actual height of the body from the ground to the top of the head during locomotion) is the limiting factor. Here, we aim to analyze the relationship between relative postural height (%stature), kinematics, and muscle activity during crouched walking.Materials and MethodsAdult males (n = 19) walked with extended limbs and at three degrees of crouch while their 3D motion capture kinematics and lower limb muscle electromyography were recorded. We measured activation of tibialis anterior, soleus, gastrocnemius medialis, gastrocnemius lateralis, vastus lateralis, rectus femoris, biceps femoris, and gluteus maximus. We analyzed the effects of postural height on kinematics and muscle activation using linear mixed effects model.ResultsFlexion angles, individual muscle activation (except for medial gastrocnemius), and total muscle activation were negatively related to relative postural height, that is, were greater at more crouched postures. Relative postural height had a stronger effect on the activation of the thigh and gluteal muscles compared to shank muscles.DiscussionGeneral increase in lower limb muscle activation at lower postural heights suggests a negative relationship between relative postural height and fatigue, and may indicate a possible mechanism by which short stature could benefit the hunter in approach hunting. Greater activation of thigh and gluteal muscles relative to shank muscles may help to identify crouched walking in past human populations.