The N-<i>ras</i>oncogene assigned to the short arm of human chromosome 1

Martinville, B de; Cunningham, James M.; Murray, Mark J.; Francke, Uta

doi:10.1093/nar/11.15.5267

Cited by 35 publications

(6 citation statements)

References 43 publications

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“…Restriction-endonuclease digested DNA samples were subjected to electrophoresis in 0.7-1% agarose gels and transferred to nitrocellulose as described (4,5,16). Prehybridization, hybridization and washing of filters under stringent conditions of 65°C in 0.1% SDS, 0.1 x SSC (1 x SSC is 0.15 M NaCl, 15 mM sodium citrate), and the 32P-labeling of DNA have been detailed (4,5,16). RNA Isolation and Blot Hybridization Total cellular RNA was prepared with guanidine-hydrochloride (17).…”

Section: Dna Blot Hybridizations and Restriction Endonuclease Analysismentioning

confidence: 99%

Amplified DNA in Y1 mouse adrenal tumor cells: isolation of cDNAs complementary to an amplified c-Ki-rasgene and localization of homologous sequences to mouse chromosome 6

George¹,

Scott

Martinville

et al. 1984

Nucl Acids Res

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We have isolated cDNA clones complementary to a c-Ki-ras cellular oncogene that is amplified in Y1 mouse adrenal tumor cells, with the amplified sequences located on double-minute chromatin bodies (DMs) and homogeneously staining chromosomal regions (HSRs). Characterization of the cDNAs included the isolation of corresponding genomic clones, Northern blot analysis of RNA, and DNA sequence analysis. Our studies demonstrate that the c-Ki-ras gene amplified in the Y1 cells is homologous to the human c-Ki-ras2 gene. We have also obtained evidence that, in addition to c-Ki-ras, at least one other transcription unit has been amplified in the mouse adrenal tumor cells. Moreover, by Southern blot analysis of Chinese hamster-mouse somatic cell hybrids, we have determined that the amplified DNA sequences associated with DMs and HSRs, including the c-Ki-ras gene, are present in normal mouse cells on chromosome 6.

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Section: Dna Blot Hybridizations and Restriction Endonuclease Analysismentioning

confidence: 99%

Amplified DNA in Y1 mouse adrenal tumor cells: isolation of cDNAs complementary to an amplified c-Ki-rasgene and localization of homologous sequences to mouse chromosome 6

George¹,

Scott

Martinville

et al. 1984

Nucl Acids Res

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“…We found a complex rearrangement of the short arm in two of the four copies of chromosome 11 present in this heteroploid cell line. In addition, numerous other chromosomes were rearranged (18). Furthermore, the t(3; 1 1) translocation found in tumor cells of a patient with hereditary renal cell carcinoma also involved a breakpoint in the short arm of 11 (region llpl5) (19).…”

Section: Presencementioning

confidence: 99%

“…Cytochalasins and colchicine derivatives have been used to study the roles of microfilaments and microtubules, respectively, in cell morphology, motility, and division (17)(18)(19)(20). These antimitotic drugs have been tested for indirect effects on the various IF systems with limited success (7,21).…”

Section: Presencementioning

confidence: 99%

Oncogene from Human EJ Bladder Carcinoma Is Located on the Short Arm of Chromosome 11

Martinville

Giacalone

Shih

et al. 1983

Science

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“…D20S4, a 1.2-kb HindIII fragment derived from human chromosome 20 (4,5), was obtained in a recombinant pBR322 plasmid from Peter O'Connell (University of Utah). The probe recognizes a two-allele Msp I restriction fragment length polymorphism (RFLP).…”

Section: Methodsmentioning

confidence: 99%

Detection of human DNA polymorphisms with a simplified denaturing gradient gel electrophoresis technique.

Noll¹,

Collins²

1987

Proc. Natl. Acad. Sci. U.S.A.

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Single base pair differences between otherwise identical DNA molecules can result in altered melting behavior detectable by denaturing gradient gel electrophoresis. We have developed a simplified procedure for using denaturing gradient gel electrophoresis to detect base pair changes in genomic DNA. Genomic DNA is digested with restriction enzymes and hybridized in solution to labeled single-stranded probe DNA. The excess probe is then hybridized to complementary phage M13 template DNA, and the reaction mixture is electrophoresed on a denaturing gradient gel. Only the genomic DNA-probe hybrids migrate into the gel. Differences in hybrid mobility on the gel indicate base pair changes in the genomic DNA. We have used this technique to identify two polymorphic sites within a 1.2-kilobase region of human chromosome 20. This approach should greatly facilitate the identification of DNA polymorphisms useful for gene linkage studies and the diagnosis of genetic diseases.The ability to identify DNA sequence heterogeneity by restriction endonuclease analysis has revolutionized gene linkage studies and genetic disease diagnosis. However, one significant limitation of this technique is that sequence differences must be present at restriction enzyme recognition sites in order to be identified in most cases. Differences occurring outside the recognition sites cannot be detected, and even large panels of restriction enzymes recognize only a fraction of the sequence heterogeneity that is present.In contrast, denaturing gradient gel electrophoresis (DGGE) allows detection of any single-base change that occurs within the long stretches of DNA that form earlymelting domains (see ref. 1 for a review of DGGE). An analysis of predicted melting domain structure for a 35-kilobase (kb) human DNA segment suggests that up to 50-70% of all base pair substitutions should be detectable (2). In the DGGE procedure, DNA molecules are held at the temperature of incipient denaturation and electrophoresed through a polyacrylamide gel containing a gradient of increasing concentrations of urea and formamide. DNA fragments moving through the gel eventually encounter a concentration of denaturants where the early melting domain becomes unstable and abruptly melts, producing molecules that are partially double-stranded and partially disordered. These partially melted molecules migrate more slowly, if at all, through the gel. The position in the gradient at which migration becomes altered is unique and characteristic for each individual fragment. Remarkably, molecules that differ by only a single base pair in the early-melting domain can be separated on these gels because of the alteration in domain melting temperature.Recently Myers et al. reported that DGGE could be used to examine known single-base mutations in the globin genes of patients with /3-thalassemia (3). Here we show that DGGE can be used to detect previously unidentified genomic DNA polymorphisms. We also describe a simple technique for reducing probe background that improves the qu...

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The N-rasoncogene assigned to the short arm of human chromosome 1

Cited by 35 publications

References 43 publications

Amplified DNA in Y1 mouse adrenal tumor cells: isolation of cDNAs complementary to an amplified c-Ki-rasgene and localization of homologous sequences to mouse chromosome 6

Amplified DNA in Y1 mouse adrenal tumor cells: isolation of cDNAs complementary to an amplified c-Ki-rasgene and localization of homologous sequences to mouse chromosome 6

Oncogene from Human EJ Bladder Carcinoma Is Located on the Short Arm of Chromosome 11

Detection of human DNA polymorphisms with a simplified denaturing gradient gel electrophoresis technique.

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