The N-terminal domain of Arabidopsis proline dehydrogenase affects enzymatic activity and protein oligomerization

Fabro, Georgina; Cislaghi, Ana Paula; Condat, Félix; Borau, Germán Deza; Alvarez, María Elena

doi:10.1016/j.plaphy.2020.04.019

Cited by 9 publications

(2 citation statements)

References 30 publications

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“…Purification of the GST:ProDH fusion proteins strongly increased the specific activity of prolinedependent DCPIP reduction, whereas the proline-dependent reduction of NAD + at pH 10 was depleted (Figure 3 and Supplementary Figure S1). The specific activities of the purified GST:ProDH1 N12 and GST:ProDH2 N13 were similar to the activities reported recently for ProDH1 N39 and ProDH2 N29 expressed as fusion proteins with the maltose binding protein (Fabro et al, 2020).…”

Section: Discussionsupporting

confidence: 86%

Appropriate Activity Assays Are Crucial for the Specific Determination of Proline Dehydrogenase and Pyrroline-5-Carboxylate Reductase Activities

et al. 2020

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Accumulation of proline is a widespread plant response to a broad range of environmental stress conditions including salt and osmotic stress. Proline accumulation is achieved mainly by upregulation of proline biosynthesis in the cytosol and by inhibition of proline degradation in mitochondria. Changes in gene expression or activity levels of the two enzymes catalyzing the first reactions in these two pathways, namely pyrroline-5-carboxylate (P5C) synthetase and proline dehydrogenase (ProDH), are often used to assess the stress response of plants. The difficulty to isolate ProDH in active form has led several researchers to erroneously report proline-dependent NAD+ reduction at pH 10 as ProDH activity. We demonstrate that this activity is due to P5C reductase (P5CR), the second and last enzyme in proline biosynthesis, which works in the reverse direction at unphysiologically high pH. ProDH does not use NAD+ as electron acceptor but can be assayed with the artificial electron acceptor 2,6-dichlorophenolindophenol (DCPIP) after detergent-mediated solubilization or enrichment of mitochondria. Seemingly counter-intuitive results from previous publications can be explained in this way and our data highlight the importance of appropriate and specific assays for the detection of ProDH and P5CR activities in crude plant extracts.

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Section: Discussionsupporting

confidence: 86%

Appropriate Activity Assays Are Crucial for the Specific Determination of Proline Dehydrogenase and Pyrroline-5-Carboxylate Reductase Activities

et al. 2020

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“…The synthesis of proline in plants is catalyzed by P5CS and P5CR. For example, P5CS plays a critical role in the synthesis of proline when plants suffer stress [38]. As the key enzyme in proline synthesis, the activity of P5CS was reinforced by NFT storage in peach fruits while the activity of P5CR was weakened, indicating that NFT accelerated proline synthesis.…”

Section: Discussionmentioning

confidence: 99%

Near-Freezing Temperature Storage Improves Peach Fruit Chilling Tolerance by Regulating the Antioxidant and Proline Metabolism

Zhao,

Meng,

et al. 2024

Horticulturae

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Chilling injury (CI) in peach fruit (Prunus persica cv. Yuhualu) is generally caused by long-time low temperature (5 °C or 0 °C) storage. However, peach fruit stored at near-freezing temperature (NFT in this research is −1 °C), defined as within 0.5 °C above the biological freezing point of biological tissue, does not exhibit CI symptoms. The effect of NFT on the CI, proline metabolism, and antioxidant capability of peach fruit during storage was studied and compared with 5 °C and 0 °C storage as controls. The results exhibit that NFT completely inhibited the occurrence of CI in peach fruit. NFT significantly (p < 0.05) enhanced the activities of superoxide dismutase, catalase, ascorbate peroxidase, and 1,1-diphenyl-2-picrylhydrazyl scavenging capacity. Moreover, the increase of malondialdehyde, ion leakage, and H2O2 accumulation were inhibited remarkably by NFT, and decreases in the contents of phenolics and ascorbic acid were slowed significantly in peach fruit stored at NFT (p < 0.05). Additionally, NFT storage enhanced proline accumulation by modulating the activity of proline metabolizing enzymes. In conclusion, the above results suggest that NFT storage can improve the chilling tolerance of peach fruit by regulating the antioxidant defense and proline metabolism, which might represent a potential novel method to store fruits and vegetables for longer storage times.

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Insight into the transcriptional regulation of key genes involved in proline metabolism in plants under osmotic stress

Yan,

Zhan,

Liu

et al. 2024

Biochimie

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The N-terminal domain of Arabidopsis proline dehydrogenase affects enzymatic activity and protein oligomerization

Cited by 9 publications

References 30 publications

Appropriate Activity Assays Are Crucial for the Specific Determination of Proline Dehydrogenase and Pyrroline-5-Carboxylate Reductase Activities

Appropriate Activity Assays Are Crucial for the Specific Determination of Proline Dehydrogenase and Pyrroline-5-Carboxylate Reductase Activities

Near-Freezing Temperature Storage Improves Peach Fruit Chilling Tolerance by Regulating the Antioxidant and Proline Metabolism

Insight into the transcriptional regulation of key genes involved in proline metabolism in plants under osmotic stress

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