The N-terminal region of organic anion transporting polypeptide 1B3 (OATP1B3) plays an essential role in regulating its plasma membrane trafficking

Chun, Se-Eun; Thakkar, Nilay; Park, Ji Eun; Han, Songhee; Hahn, Hyunggu; Maeng, Sang Hyun; Lim, Young-Ran; Han, Byung Woo; Lee, Wooin

doi:10.1016/j.dmpk.2016.10.397

Cited by 1 publication

(1 citation statement)

References 24 publications

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“…However, transporter proteins resident in the plasma membrane are preferentially quantified in purified fractions compared with total transporter protein, which can also be distributed into intracellular membranes. Plasma membrane fractionation techniques work under the assumption that only proteins localized on the plasma membrane at the time of extraction are available to carry out the relevant activity …”

Section: Sample Preparation Approachesmentioning

confidence: 99%

Toward a Consensus on Applying Quantitative Liquid Chromatography‐Tandem Mass Spectrometry Proteomics in Translational Pharmacology Research: A White Paper

Prasad

Achour

Artursson

et al. 2019

Clin Pharma and Therapeutics

129

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Quantitative translation of information on drug absorption, disposition, receptor engagement, and drug–drug interactions from bench to bedside requires models informed by physiological parameters that link in vitro studies to in vivo outcomes. To predict in vivo outcomes, biochemical data from experimental systems are routinely scaled using protein quantity in these systems and relevant tissues. Although several laboratories have generated useful quantitative proteomic data using state‐of‐the‐art mass spectrometry, no harmonized guidelines exit for sample analysis and data integration to in vivo translation practices. To address this gap, a workshop was held on September 27 and 28, 2018, in Cambridge, MA, with 100 experts attending from academia, the pharmaceutical industry, and regulators. Various aspects of quantitative proteomics and its applications in translational pharmacology were debated. A summary of discussions and best practices identified by this expert panel are presented in this “White Paper” alongside unresolved issues that were outlined for future debates.

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Section: Sample Preparation Approachesmentioning

confidence: 99%