The NAM1/MTF2 nuclear gene product is selectively required for the stability and/or processing of mitochondrial transcripts of the atp6 and of the mosaic, cox1 and cytb genes in Saccharomyces cerevisiae

Groudinsky, Olga; Bousquet, Isabelle; Wallis, Mary G.; Słonimski, Piotr P.; Dujardin, Geneviève

doi:10.1007/bf00280396

Cited by 39 publications

(4 citation statements)

References 36 publications

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“…In this scenario, interaction of the HN31 product with the tscA/chlN precursor would establish a secondary structure necessary for tscA 3′ end processing, whereas binding to the second psaA intron would stabilize the conserved group II intron structure, and thus allow exon 2‐exon 3‐splicing. Similar bifunctional RNA‐binding proteins have been found in yeast and Neurospora ( Akins & Lambowitz 1987;Groudinsky et al . 1993 ;Herbert et al .…”

Section: Resultssupporting

confidence: 65%

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A single nuclear locus is involved in both chloroplast RNAtrans‐splicing and 3′ end processing

et al. 1998

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SummaryThe complex maturation of the chloroplast psaA mRNA in Chlamydomonas reinhardtii requires the splicing of three separate exons in trans. At least 14 nuclear gene products and a small chloroplast RNA (tscA) seem to be involved in psaA trans-splicing. To further elucidate the mechanisms that regulate trans-splicing in C. reinhardtii, we have analyzed four different nuclear mutants; two affected in trans-splicing of the psaA exons 1 and 2 (class C) and two blocked in both psaA trans-splicing steps (class B). While mutants M18 (class C) and L118 (class B) have a mature chloroplast tscA RNA, no such transcripts could be detected in mutants TR72 (class C) and HN31 (class B). Northern hybridizations indicate that the absence of mature tscA transcripts in HN31 and TR72 results from a defect in the processing of a tscA/chlN precursor transcript. Since the tscA locus has been shown to mediate psaA exon 1 and 2 trans-splicing, the nucleus-encoded factor affected in the class C mutant TR72 may be required for tscA 3Ј end maturation and only indirectly for psaA exon 1 and 2 trans-splicing. Moreover, further genetic analysis revealed that the nuclear HN31 locus is involved in both maturation of the tscA/chlN precursor and transsplicing of the psaA exons 2 and 3.

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Section: Resultssupporting

confidence: 65%

“…1988 ). The NAM1 nuclear gene product of S. cerevisiae , for instance, is supposed to be a stem‐loop specific RNA‐binding protein required for splicing as well as for processing and/or stability of different mitochondrial transcripts ( Groudinsky et al . 1993 ).…”

Section: Resultsmentioning

confidence: 99%

A single nuclear locus is involved in both chloroplast RNAtrans‐splicing and 3′ end processing

et al. 1998

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“…Besides, the increase in liver content of COI and cyt b mRNAs observed during the first hour post-natal ( Figure 2 and Table 1) suggests the existence of specific stabilization of these transcripts during organelle differentiation. In the specific case of COI and cyt b mRNAs, it seems reasonable to suggest that the mammalian homologue of the protein product of the yeast nuclear gene NAM1 [45,46] could perform such a task. However, although cyt b and COI mRNAs significantly increase in the neonatal liver ( Figure 2 and Table 1), the relative amount of their translated products does not change in parallel (Figure 4), which might suggest that stabilization of these transcripts affects their translation (see below).…”

Section: Replication and Transcription Of Mt-dna During Liver Developmentioning

confidence: 99%

Transient activation of mitochondrial translation regulates the expression of the mitochondrial genome during mammalian mitochondrial differentiation

Ostronoff

Izquierdo

Enríquez

et al. 1996

Biochemical Journal

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Regulation of the expression of the nuclear-encoded beta-subunit of H(+)-ATP synthase (beta-F1-ATPase) gene of oxidative phosphorylation during differentiation of liver mitochondria is mainly exerted at two post-transcriptional levels affecting both the half-life [Izquierdo, Ricart, Ostronoff, Egea and Cuezva (1995) J. Biol. Chem. 270, 10342-10350] and translational efficiency [Luis, Izquierdo, Ostronoff, Salinas, Santarén and Cuezva (1993) J. Biol. Chem. 268, 1868-1875] of the transcript. Herein, we have studied the expression of the mitochondrial (mt) genome during differentiation of rat liver mitochondria in an effort to elucidate the mechanisms of nucleo-mitochondrial cross-talk during biogenesis of the organelle. Estimation of the relative cellular representation of met-DNA in liver reveals a negligible increase in mt-DNA copy number during organelle differentiation. Concurrently, the lack of changes in transcription rates of the mt-DNA "in organello', as well as in steady-state levels of the mt-transcripts, suggests that organelle differentiation is not controlled by an increase in transcription of the mt-genome. However, translation rates in isolated mitochondria revealed a transient 2-fold increase immediately after birth. Interestingly, the transient activation of mitochondrial translation at this stage of liver development is dependent on the synthesis of proteins in cytoplasmic polyribosomes. These findings support the hypothesis that the expression of nuclear and mitochondrial genes during biogenesis of mammalian mitochondria is developmentally regulated by a post-transcriptional mechanism that involves concerted translational control of both genomes.

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“…Still far too little is known about the mitochondrial degradosome – a complex responsible for the 3′‐5′ degradation of almost all mRNAs and containing Suv3p and Dss1p together with the product of a third as yet unidentified gene [6]. The same can be said of a number of factors, which have undefined roles in mRNA‐specific stabilisation, sometimes with additional (and undefined) roles in other processes like transcription (Nam1p/Mtf 2 p [7]), processing (Cbp1p, Pet127p [8,9]), or translation (Pet309p [10])…”

Section: Processing Splicing and Stabilisation Of Mrnasmentioning

confidence: 99%

Mitochondrial assembly in yeast

Grivell¹,

Artal‐Sanz²,

Hakkaart³

et al. 1999

FEBS Letters

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The yeast Saccharomyces cerevisiae is likely to be the first organism for which a complete inventory of mitochondrial proteins and their functions can be drawn up. A survey of the 340 or so proteins currently known to be localised in yeast mitochondria reveals the considerable investment required to maintain the organelle's own genetic system, which itself contributes seven key components of the electron transport chain. Translation and respiratory complex assembly are particularly expensive processes, together requiring around 150 of the proteins so far known. Recent developments in both areas are reviewed and approaches to the identification of novel mitochondrial proteins are discussed.z 1999 Federation of European Biochemical Societies.

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The NAM1/MTF2 nuclear gene product is selectively required for the stability and/or processing of mitochondrial transcripts of the atp6 and of the mosaic, cox1 and cytb genes in Saccharomyces cerevisiae

Cited by 39 publications

References 36 publications

A single nuclear locus is involved in both chloroplast RNAtrans‐splicing and 3′ end processing

A single nuclear locus is involved in both chloroplast RNAtrans‐splicing and 3′ end processing

Transient activation of mitochondrial translation regulates the expression of the mitochondrial genome during mammalian mitochondrial differentiation

Mitochondrial assembly in yeast

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