The nanos gene of Bombyx mori and its expression patterns in developmental embryos and larvae tissues

Zhao, Guoli; Chen, Keping; Yao, Qin; Wang, Weihua; Wang, Yong; Mu, Runhong; Chen, Huiqing; Yang, Huajun; Zhou, Hongliang

doi:10.1016/j.gep.2007.12.006

Cited by 14 publications

(10 citation statements)

References 32 publications

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“…For instance, the vasa gene, originally identified in Drosophila , is essential for abdomen development in early embryogenesis [39], and has been identified as a marker gene of germ cells [40]. The nanos gene is a core component of germplasm and is involved in the formation of anterior-posterior body axis during embryogenesis [41]. One of maternal genes, Mago nashi , is required for germ cell determination and delineation of the longitudinal axis of the embryo [42].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Characterization of Dendrolimus punctatus and Expression Profiles at Different Developmental Stages

Yang

et al. 2016

PLoS ONE

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The pine moth Dendrolimus punctatus (Walker) is a common insect pest that confers serious damage to conifer forests in south of China. Extensive physiology and ecology studies on D. punctatus have been carried out, but the lack of genetic information has limited our understanding of the molecular mechanisms behind its development and resistance. Using RNA-seq approach, we characterized the transcriptome of this pine moth and investigated its developmental expression profiles during egg, larval, pupal, and adult stages. A total of 107.6 million raw reads were generated that were assembled into 70,664 unigenes. More than 30% unigenes were annotated by searching for homology in protein databases. To better understand the process of metamorphosis, we pairwise compared four developmental phases and obtained 17,624 differential expression genes. Functional enrichment analysis of differentially expressed genes showed positive correlation with specific physiological activities of each stage, and these results were confirmed by qRT-PCR experiments. This study provides a valuable genomic resource of D. punctatus covering all its developmental stages, and will promote future studies on biological processes at the molecular level.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Characterization of Dendrolimus punctatus and Expression Profiles at Different Developmental Stages

Yang

et al. 2016

PLoS ONE

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“…However, nanos genes in invertebrates were likely to have similar expression pattern. In B. mori, RT-PCR and Western Blot analysis confirmed that nanos was expressed in diverse somatic tissue, such as fat body, blood, midgut and so on (Zhao et al, 2008). Similarly, during early cleavage stages of polychaete Capitella sp.…”

Section: Discussionmentioning

confidence: 86%

“…In the ovary of B. mori larvae, Nanos proteins were primarily localized in oocytes. Moreover, co-localization of Vasa and Nanos further revealed that Nanos was a germline marker in silkworm (Zhao et al, 2008). Besides, in Botryllus primigenus, nanos was a strong marker of spermatogonia and spermatocytes, although it was also detected in the somatic tissues (Sunanaga et al, 2008).…”

Section: Discussionmentioning

confidence: 93%

“…In addition to its germline functions, nanos was expressed widely in multipotent cells and somatic tissues. The homologue of nanos gene was identified in diverse invertebrates, such as Nematostella vectensis (Extavour et al, 2005), Ilyanassa obsoleta (Rabinowitz et al, 2008), Hydra magnipapillata (Mochizuki et al, 2000), Platynereis dumerilii (Rebscher et al, 2008), Bombyx mori (Zhao et al, 2008), Hemicentrotus pulcherrimus (Fujii et al, 2006), Strongylocentrotus purpuratus and so on. In sea anemone, nanos gene was expressed in multiple somatic types during early embryogenesis (Extavour et al, 2005), while Hydra nanos was expressed in multipotent interstitial cells, which produced both several somatic cell types and germ cells (Mochizuki et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

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Molecular and expression characterization of a nanos1 homologue in Chinese sturgeon, Acipenser sinensis

Chen

Wei

et al. 2012

Gene

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“…The presence of nanos-related genes occurs in a great variety of other taxa and is associated with the germline (Zhao et al 2008;Dearden 2006;Calvo et al 2005;Juhn et al 2008). Some mosquitoes are vectors of parasitic and viral pathogens.…”

Section: Communicated By S Rothmentioning

confidence: 99%

Rananos expression pattern during oogenesis and early embryonic development in Rhynchosciara americana

2012

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The Dipteran Rhynchosciara americana, a native Brazilian insect that has become a valuable model system for developmental biology research because it provides an interesting opportunity to study a different type of insect oogenesis. Sequences from a cDNA library that was constructed with poly A+RNA from the ovaries of R. americana larvae at different ages were analyzed. Molecular characterization confirmed interesting findings, such as the presence of Rananos. The nanos gene encodes a conserved RNA-binding protein that is required during early development for the maintenance and division of the primordial germ cells of Diptera. nanos plays an important role in specifying the posterior regions of insect embryos and is important for abdomen formation. In the present work, we showed the spatial and temporal expression profiles of this important gene, which is involved in oogenesis and early development. Data mining techniques were used to obtain the complete sequence of Rananos. Bioinformatic tools were used to determine the following: (1) the secondary structure of the 3'-untranslated region of the Rananos mRNA, (2) the encoded protein of the isolated Rananos gene, (3) the conserved zinc-finger domains of the RaNanos protein, and (4) phylogenetic analyses. Furthermore, RNA in situ hybridization and immunolocalization were used to determine mRNA and protein expression in the tissues that were studied and to define Rananos as a germ cell molecular marker.

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The nanos gene of Bombyx mori and its expression patterns in developmental embryos and larvae tissues

Cited by 14 publications

References 32 publications

Transcriptome Characterization of Dendrolimus punctatus and Expression Profiles at Different Developmental Stages

Transcriptome Characterization of Dendrolimus punctatus and Expression Profiles at Different Developmental Stages

Molecular and expression characterization of a nanos1 homologue in Chinese sturgeon, Acipenser sinensis

Rananos expression pattern during oogenesis and early embryonic development in Rhynchosciara americana

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