The Nanostructured lipid carrier gel of Oroxylin A reduced UV-induced skin oxidative stress damage

Zhushan,; Zhao, Zhiyue; Qin, Wenxiao; Yang, Yi; Wang, Zijing; Ma, Hongfei; Wang, Xiang; Liu, Tao; Qi, Dongli; Guo, Pan; Pi, Jiaxin; Tian, Baocheng; Zhang, Han; Li, Nan

doi:10.1016/j.colsurfb.2022.112578

Cited by 17 publications

(2 citation statements)

References 61 publications

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“…Skin photoaging is a complicated process in which skin changes are caused by persistent ultraviolet (UV) exposure [1][2][3]. Epidemiological studies and surveys have shown that skin photoaging contributes to the development of numerous skin disorders and skin tumors [4,5].…”

Section: Introductionmentioning

confidence: 99%

Needle-Free Injection of Metformin Ameliorates Skin Photoaging Through Inhibition of Ferroptosis and Oxidative Stress

Zhang,

Qin,

Zhang

et al. 2024

Discovery Medicine

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Background: Skin photoaging is a complex process of skin aging caused by continuous exposure to ultraviolet (UV) radiation through oxidative stress and other pathways, yet effective treatments are scarce. Metformin is a drug with both anti-senescence and antioxidant functions; however, there are fewer studies on photoaging. The study aimed to investigate the role of needlefree injection of metformin in alleviating ultraviolet radiation B (UVB) induced skin photoaging, and to explore the mechanisms through which metformin alleviates fibroblast photoaging by inhibiting ferroptosis and oxidative stress. Methods: In our study, we initially performed bioinformatic analysis on the gene expression profile (GSE38308), and our RNA sequencing (RNA-Seq) found that photoaging is associated with ferroptosis. We investigated the potential skin-protective mechanism of metformin by utilizing a UVB-induced rat skin photoaging model and human skin fibroblasts (HSF) treated with UVB. For in vitro experiments, cellular senescence was detected using SA-β-galactosidase staining and p16 in western blot. Ferroptosis and oxidative stress were assessed via western blot (glutathione Peroxidase 4 (GPX4) and nuclear factor erythroid-2-related factor 2 (Nrf2)), reactive oxygen species (ROS) levels, transmission electron microscope, Lillie's staining, and immunofluorescence staining. During in vivo experiments, metformin was administered by needle-free jet injectors injected into the backs of rats. The effectiveness of metformin was detected using the Masson staining and western blot. Results: We found that the ferroptosis pathway was closely associated with photoaging through bioinformatics analysis. In the UVB-induced photoaging HSF cells, treatment with metformin exhibits the following effects: a reduction in blue-stained granules in SA-β-galactosidase staining and a decrease in the expression of p16, indicating a reduction in cellular senescence. Moreover, metformin leads to decreased ROS levels and increased expression of the oxidative stress-related protein Nrf2, suggesting inhibition of oxidative stress within the cells. Additionally, metformin results in an elevation of GPX4 expression, a decrease in blue-stained granules in Lillie's staining, and a reduction in ferroptosis-associated mitochondrial damage, indicating a decline in ferroptosis. Needle-free injection of metformin could directly achieve therapeutic effects by affecting HSF cells in the dermis. The needle-free injection of metformin treatment effectively improved the photoaging skin in rats compared to the photoaging group, ameliorated oxidative stress, and reduced ferroptosis. Conclusions: Our data highlights a novel needle-free injection of metformin that improves photoaging and has good therapeutic potential.

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Section: Introductionmentioning

confidence: 99%

Needle-Free Injection of Metformin Ameliorates Skin Photoaging Through Inhibition of Ferroptosis and Oxidative Stress

Zhang,

Qin,

Zhang

et al. 2024

Discovery Medicine

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“…Moreover, our previous studies had revealed that corylin from Psoralea corylifolia L. and Oroxylin A from Eucommia ulmoides Oliv. inhibit oxidative stress by activating Nrf2 to prevent UV-induced damage ( Zhao et al, 2021 ; Li et al, 2022 ; Zhu et al, 2022 ). However, whether MQEF could prevent UV radiation-induced photoaging remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Modified Qing’e Formula protects against UV-induced skin oxidative damage via the activation of Nrf2/ARE defensive pathway

et al. 2022

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Exposure to ultraviolet (UV) light triggers the rapid generation and accumulation of reactive oxygen species (ROS) in skin cells, which increases oxidative stress damage and leads to photoaging. Nuclear factor E2-related factor 2 (Nrf2) modulates the antioxidant defense of skin cells against environmental factors, especially ultraviolet radiation. Natural products that target Nrf2-regulated antioxidant reactions are promising candidates for anti-photoaging. The aim of this study was to investigate the protective effect of Modified Qing’e Formula (MQEF) on UV-induced skin oxidative damage and its molecular mechanisms. In this study, the photoaging models of human keratinocytes (HaCaT) and ICR mice were established by UV irradiation. In vitro models showed that MQEF displayed potent antioxidant activity, significantly increased cell viability and reduced apoptosis and excess ROS levels. Meanwhile, the knockdown of Nrf2 reversed the antioxidant and anti-apoptotic effects of MQEF. In vivo experiments indicated that MQEF could protect the skin against UV-exposed injury which manifested by water loss, sensitivity, tanning, wrinkling, and breakage of collagen and elastic fibers. The application of MQEF effectively increased the activity of antioxidant enzymes and reduced the content of malondialdehyde (MDA) in mice. In addition, MQEF was able to activate Nrf2 nuclear translocation in mouse skin tissue. In summary, MQEF may attenuate UV-induced photoaging by upregulating Nrf2 expression and enhancing antioxidant damage capacity. MQEF may be a potential candidate to prevent UV-induced photoaging by restoring redox homeostasis.

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Antimicrobial and Antioxidant Hydrogels Based on the Water‐Insoluble β‐Glucan From Poria cocos Loaded With Total Flavonoids of Ampelopsis grossedentata

Wang,

et al. 2024

ChemistrySelect

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This study aims to develop and assess an antimicrobial hydrogel that provides a sanitizer effect while avoiding skin oxidative damage. The hydrogel is composed of Poria cocos polysaccharide and loaded with total flavonoids extracted from Ampelopsis grossedentata (TF@PCPA‐G). The antibacterial activity of TF@PCPA‐G was assessed through minimum inhibitory concentration (MIC), zone of inhibition, and SYTOX green staining measurements. The findings indicate that TF@PCPA‐G exhibited remarkable antibacterial potency. Additionally, the antioxidant properties of the hydrogel were examined using DPPH, ABTS, and hydroxyl radical scavenging assay models, revealing that TF@PCPA‐G possessed a persistent ability to neutralize free radicals. Subsequently, the antioxidant properties of TF@PCPA‐G were further investigated using cellular and animal models of oxidative damage. As anticipated, TF@PCPA‐G effectively alleviated oxidative stress in cells and animal skin. In conclusion, TF@PCPA‐G has been demonstrated to eliminate bacteria effectively and prevent skin damage.

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The Nanostructured lipid carrier gel of Oroxylin A reduced UV-induced skin oxidative stress damage

Cited by 17 publications

References 61 publications

Needle-Free Injection of Metformin Ameliorates Skin Photoaging Through Inhibition of Ferroptosis and Oxidative Stress

Needle-Free Injection of Metformin Ameliorates Skin Photoaging Through Inhibition of Ferroptosis and Oxidative Stress

Modified Qing’e Formula protects against UV-induced skin oxidative damage via the activation of Nrf2/ARE defensive pathway

Antimicrobial and Antioxidant Hydrogels Based on the Water‐Insoluble β‐Glucan From Poria cocos Loaded With Total Flavonoids of Ampelopsis grossedentata

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