The Native Monomer of Bacillus Pumilus Ribonuclease Does Not Exist Extracellularly

Ilinskaya, О. N.; Ulyanova, Vera; Lisevich, Irina; Dudkina, Elena; Zakharchenko, N. L.; Kusova, Aleksandra M.; Faizullin, Dzhigangir A.; Zuev, Yu. F.

doi:10.1155/2018/4837623

Cited by 17 publications

(6 citation statements)

References 44 publications

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“…Secretion of enzymes including RNases, which participate in mobilization of organic phosphates, is one of the mechanisms of bacterial adaptation to changing environmental conditions. The ability of Bacillus pumilus , Bacillus amyloliquefaciens , and Bacillus licheniformis to produce extracellular RNases (binases, baRNases, and baliphases, respectively) is actively investigated ( Ulyanova et al, 2016 ; Ilinskaya et al, 2018 ). Low concentrations of RNases stimulate plant growth and resistance to a broad spectrum of stress factors, and high levels of them show antiviral properties by destroying viral RNA.…”

Section: Introductionmentioning

confidence: 99%

Endophytic Bacillus spp. as a Prospective Biological Tool for Control of Viral Diseases and Non-vector Leptinotarsa decemlineata Say. in Solanum tuberosum L.

et al. 2020

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Viral diseases and their damage causing significant loss to economically important crops have increased by several folds during the last decade. All the conventional approaches are not able to eradicate the viral infection. Therefore, there is a need to look for efficient and eco-friendly viral disease-preventive measures. The genomic material of the majority of deleterious viruses of higher plants is RNA. One of the possible measures to control viruses is the use of ribonucleases (RNases), which can cleave RNA in the viral genome. Based on this, we investigated the RNase activity of endophytic Bacillus spp., which can enrich in 10 3 –10 5 colony-forming units per gram of wet mass of aboveground part of potato plants. A high level of RNase activity was observed in the culture medium of Bacillus thuringiensis B-6066, Bacillus sp. STL-7, Bacillus sp. TS2, and Bacillus subtilis 26D. B. thuringiensis B-5351 had low RNase activity but high ability to colonize internal plant tissues, Bacillus sp. STL-7 with high RNase activity have relatively low number of cells in internal tissues of plants. B. thuringiensis B-6066, B. subtilis 26D, and Bacillus sp. TS stimulate RNase activity in potato plants for a long time after application. Strains with high ability to colonize internal plant tissues combined with high RNase activity reduced severity of viral diseases symptoms on plants and reduced the incidence of potato viruses M, S, and Y. It is worth noting that Bacillus spp. under investigation reduced the number of Leptinotarsa decemlineata Say. egg clusters and larvae on treated plants and showed antifeedant activity. This results in increase of potato productivity mainly in the fraction of major tubers. B. subtilis 26D and Bacillus sp. TS2 combining endophytic lifestyle, RNase, and antifeedant activity may become the basis for the development of biocontrol agents for plant protection.

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Section: Introductionmentioning

confidence: 99%

Endophytic Bacillus spp. as a Prospective Biological Tool for Control of Viral Diseases and Non-vector Leptinotarsa decemlineata Say. in Solanum tuberosum L.

et al. 2020

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“…This method is helpful to detect aggregates in macromolecular solutions, to determine the size of proteins and to monitor the binding of ligands. The hydrodynamic diameter of binase and balifase was found to be 3.67 nm and 3.25 nm, respectively (Figure 3), while the calculated size of their monomers is 1.6 nm [44]. Therefore, these RNases are represented in solution mainly by dimeric forms.…”

Section: Structural Characterization Of Homologous Rnasesmentioning

confidence: 94%

Structural and Functional Differences between Homologous Bacterial Ribonucleases

Ulyanova

Nadyrova

Dudkina

et al. 2022

IJMS

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Small cationic guanyl-preferring ribonucleases (RNases) produced by the Bacillus species share a similar protein tertiary structure with a high degree of amino acid sequence conservation. However, they form dimers that differ in conformation and stability. Here, we have addressed the issues (1) whether the homologous RNases also have distinctions in catalytic activity towards different RNA substrates and interactions with the inhibitor protein barstar, and (2) whether these differences correlate with structural features of the proteins. Circular dichroism and dynamic light scattering assays revealed distinctions in the structures of homologous RNases. The activity levels of the RNases towards natural RNA substrates, as measured spectrometrically by acid-soluble hydrolysis products, were similar and decreased in the row high-polymeric RNA >>> transport RNA > double-stranded RNA. However, stopped flow kinetic studies on model RNA substrates containing the guanosine residue in a hairpin stem or a loop showed that the cleavage rates of these enzymes were different. Moreover, homologous RNases were inhibited by the barstar with diverse efficiency. Therefore, minor changes in structure elements of homologous proteins have a potential to significantly effect molecule stability and functional activities, such as catalysis or ligand binding.

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“…Currently, more than 20 extracellular RNases of Bacillus have been discovered. For example, B. amyloliquefaciens, B. pumilus, and B. licheniformis produce extracellular RNases called barnases, binases, and baliphases, respectively [63,64]. Among endophytic microorganisms isolated from diverse cultivated Cucurbitaceae, 73% of Bacillus, 27% of Paenibacillus, and 30% of Enterobacteriaceae isolates, as well as all Cronobacter, Pantoea, Microbacterium, and Staphylococcus isolates displayed RNase activity [65].…”

Section: Bacterial Nucleases and Their Antiviral Activitymentioning

confidence: 99%

Mechanisms of Plant Tolerance to RNA Viruses Induced by Plant-Growth-Promoting Microorganisms

Максимов

Sorokan

Бурханова

et al. 2019

Plants

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Plant viruses are globally responsible for the significant crop losses of economically important plants. All common approaches are not able to eradicate viral infection. Many non-conventional strategies are currently used to control viral infection, but unfortunately, they are not always effective. Therefore, it is necessary to search for efficient and eco-friendly measures to prevent viral diseases. Since the genomic material of 90% higher plant viruses consists of single-stranded RNA, the best way to target the viral genome is to use ribonucleases (RNase), which can be effective against any viral disease of plants. Here, we show the importance of the search for endophytes with protease and RNase activity combined with the capacity to prime antiviral plant defense responses for their protection against viruses. This review discusses the possible mechanisms used to suppress a viral attack as well as the use of local endophytic bacteria for antiviral control in crops.

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The Native Monomer of Bacillus Pumilus Ribonuclease Does Not Exist Extracellularly

Cited by 17 publications

References 44 publications

Endophytic Bacillus spp. as a Prospective Biological Tool for Control of Viral Diseases and Non-vector Leptinotarsa decemlineata Say. in Solanum tuberosum L.

Endophytic Bacillus spp. as a Prospective Biological Tool for Control of Viral Diseases and Non-vector Leptinotarsa decemlineata Say. in Solanum tuberosum L.

Structural and Functional Differences between Homologous Bacterial Ribonucleases

Mechanisms of Plant Tolerance to RNA Viruses Induced by Plant-Growth-Promoting Microorganisms

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