The natural antisense transcript HAS2-AS1 regulates breast cancer cells aggressiveness independently from hyaluronan metabolism

Parnigoni, Arianna; Caon, Ilaria; Teo, Wei Xuan; Hua, San Hue; Moretto, Paola; Bartolini, Barbara; Viola, Manuela; Karousou, Evgenia; Yip, George W.; Götte, Martin; Heldin, Paraskevi; Passi, Alberto; Vigetti, Davide

doi:10.1016/j.matbio.2022.03.009

Cited by 16 publications

(11 citation statements)

References 82 publications

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“…On the contrary, the transfection of HAS2 siRNA can inhibit the development of breast cancer and the occurrence of metastatic colonization through the EGF-mediated kinase/PI3K/Akt signalling pathway [ 65 ]. In contrast, suppression of HAS2 by using antisense-transcript HAS2-AS1 has been shown to attenuate the proliferation and malignant phenotype of glioma, osteosarcoma and breast cancer [ 66 – 68 ]. Furthermore, in the analysis of gene expression profiles of pancreatic cancer cells and fibroblasts, HAS2 was highly expressed as a differential expression gene and was associated with increased invasiveness of pancreatic cancer cells [ 69 ].…”

Section: Property and Variety Of Ha Under Different Microenvironmentmentioning

confidence: 99%

“…The amino acid abbreviation in white color with red background presents the aligned sequences are identical, and words in red color present the aligned sequences have similar hydrophobicity, hydrophilicity or similar side chain. Word in black presents the aligned sequences that are not conservation suppression of HAS2 by using antisense-transcript HAS2-AS1 has been shown to attenuate the proliferation and malignant phenotype of glioma, osteosarcoma and breast cancer [66][67][68]. Furthermore, in the analysis of gene expression profiles of pancreatic cancer cells and fibroblasts, HAS2 was highly expressed as a differential expression gene and was associated with increased invasiveness of pancreatic cancer cells [69].…”

Section: Ha Bio-synthesismentioning

confidence: 99%

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‘Two-faces’ of hyaluronan, a dynamic barometer of disease progression in tumor microenvironment

Liu

Wang

et al. 2023

Discov Onc

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Hyaluronan (HA) is a linear polysaccharide consisting of disaccharide units which are the d-glucuronic acid and n-acetyl-d-glucosamine. As the largest component of the extracellular matrix in microenvironment, HA polymers with different molecular weights vary in properties to molecular biology function. High molecular weight HA (HMW-HA) is mainly found in normal tissue or physiological condition, and exhibits lubrication and protection properties due to its good water retention and viscoelasticity. On the other hand, an increase in HA catabolism leads to the accumulation of low molecular weight HA (LMW-HA) under pathological circumstances such as inflammation, pre-cancerous and tumor microenvironment. LMW-HA acts as extracellular signals to enhance tumorigenic and metastatic phenotype, such as energy reprogramming, angiogenesis and extracellular matrix (ECM) remodeling. This review discusses the basic properties of this simplest carbohydrate molecule in ECM with enormous potential, and its regulatory role between tumorigenesis and microenvironmental homeostasis. The extensive discoveries of the mechanisms underlying the roles of HA in various physiological and pathological processes would provide more information for future research in the fields of biomimetic materials, pharmaceutical and clinical applications.

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Section: Property and Variety Of Ha Under Different Microenvironmentmentioning

confidence: 99%

Section: Ha Bio-synthesismentioning

confidence: 99%

‘Two-faces’ of hyaluronan, a dynamic barometer of disease progression in tumor microenvironment

Liu

Wang

et al. 2023

Discov Onc

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“…Zhao et al demonstrated that LINC02310 significantly promoted the growth and proliferation of LUAD [ 24 ]. Experiments showed that HAS2-AS1 overexpression strongly reduced breast cancer cell viability, migration and invasion [ 25 ]. This is consistent with our study results.…”

Section: Discussionmentioning

confidence: 99%

Prognostic value and immune landscapes of anoikis-associated lncRNAs in lung adenocarcinoma

Wu,

Zhang,

Feng

et al. 2024

Aging

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Background: Methods for predicting the outcome of lung adenocarcinoma (LUAD) in the clinic are limited. Anoikis is an important route to programmed cell death in LUAD, and the prognostic value of a model constructed with anoikis-related lncRNAs (ARlncRNAs) in LUAD is unclear. Methods: Transcriptome and basic information for LUAD patients was obtained from the Cancer Genome Atlas. Coexpression and Cox regression analyses were utilized to identify prognostically significant ARlncRNAs and construct a prognostic signature. Furthermore, the signature was combined with clinical characteristics to create a nomogram. Finally, we performed principal component, enrichment, tumor mutation burden (TMB), tumor microenvironment (TME) and drug sensitivity analyses to evaluate the basic research and clinical merit of the signature. Results: The prognostic signature developed with eleven ARlncRNAs can accurately predict that high-risk group patients have a worse prognosis, as proven by the receiver operating characteristic (ROC) curve (AUC: 0.718). Independent prognostic analyses indicated that the risk score is a significant independent prognostic element for LUAD (P<0.001). In the high-risk group, enrichment analysis demonstrated that glucose metabolism and DNA replication were the main enrichment pathways. TMB analysis indicated that the high-risk group had a high TMB (P<0.05). Drug sensitivity analyses can recognize drugs that are sensitive to different risk groups. Finally, 11 ARlncRNAs of this signature were verified by RT-qPCR analysis. Conclusions: A novel prognostic signature developed with 11 ARlncRNAs can accurately predict the OS of LUAD patients and offer clinical guidance value for immunotherapy and chemotherapy treatment.

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“…At present, most NATs are lncRNAs, which can directly interact with sense strand transcripts and regulate the expression of sense strand genes at the transcriptional and epigenetic levels, thus participating in various biological processes, which is of great significance to the growth and development of organisms [ 21 , 22 ]. Studies have shown that approximately 20% of the genes in the human cell transcriptome can produce NATs [ 23 ], and more than 70% of the transcription units in the mouse genome can produce antisense transcripts [ 3 , 24 ].…”

Section: Discussionmentioning

confidence: 99%

Identification cloning and functional analysis of novel natural antisense lncRNA CFL1-AS1 in cattle

Zhao

et al. 2023

Epigenetics

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Long noncoding RNAs have been identified as important regulators of gene expression and animal development. The expression of natural antisense transcripts (NATs) transcribed in the opposite direction to protein-coding genes is usually positively correlated with the expression of homologous sense genes and is the key factor for expression. Here, we identified a conserved noncoding antisense transcript, CFL1-AS1, that plays an important role in muscle growth and development. CFL1-AS1 overexpression and knockout vectors were constructed and transfected into 293T and C2C12 cells. CFL1-AS1 positively regulated CFL1 gene expression, and the expression of CFL2 was also downregulated when CFL1-AS1 was knocked down. CFL1-AS1 promoted cell proliferation, inhibited apoptosis and participated in autophagy. This study expands the research on NATs in cattle and lays a foundation for the study of the biological function of bovine CFL1 and its natural antisense chain transcript CFL1-AS1 in bovine skeletal muscle development. The discovery of this NAT can provide a reference for subsequent genetic breeding and data on the characteristics and functional mechanisms of NATs.

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The natural antisense transcript HAS2-AS1 regulates breast cancer cells aggressiveness independently from hyaluronan metabolism

Cited by 16 publications

References 82 publications

‘Two-faces’ of hyaluronan, a dynamic barometer of disease progression in tumor microenvironment

‘Two-faces’ of hyaluronan, a dynamic barometer of disease progression in tumor microenvironment

Prognostic value and immune landscapes of anoikis-associated lncRNAs in lung adenocarcinoma

Identification cloning and functional analysis of novel natural antisense lncRNA CFL1-AS1 in cattle

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