The natural environment as a reservoir of pathogenic and non-pathogenic <i>Acinetobacter</i> species

Adewoyin, Mary Ayobami; Okoh, Anthony I.

doi:10.1515/reveh-2017-0034

Cited by 48 publications

(27 citation statements)

References 67 publications

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“…Acinetobacter baumannii ( A. baumannii ) has an inevitable ability to sustain harsh conditions and spread as a serious pathogen 1 – 4 . A. baumannii infects moist tissues such as mucous membranes or areas of the skin that are exposed to this pathogen, either through wound or injury 5 , 6 .…”

Section: Introductionmentioning

confidence: 99%

Antibiofilm and antivirulence potential of silver nanoparticles against multidrug-resistant Acinetobacter baumannii

Hetta

Al-Kadmy

Khazaal

et al. 2021

Sci Rep

106

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We aimed to isolate Acinetobacter baumannii (A. baumannii) from wound infections, determine their resistance and virulence profile, and assess the impact of Silver nanoparticles (AgNPs) on the bacterial growth, virulence and biofilm-related gene expression. AgNPs were synthesized and characterized using TEM, XRD and FTIR spectroscopy. A. baumannii (n = 200) were isolated and identified. Resistance pattern was determined and virulence genes (afa/draBC, cnf1, cnf2, csgA, cvaC, fimH, fyuA, ibeA, iutA, kpsMT II, PAI, papC, PapG II, III, sfa/focDE and traT) were screened using PCR. Biofilm formation was evaluated using Microtiter plate method. Then, the antimicrobial activity of AgNPs was evaluated by the well-diffusion method, growth kinetics and MIC determination. Inhibition of biofilm formation and the ability to disperse biofilms in exposure to AgNPs were evaluated. The effect of AgNPs on the expression of virulence and biofilm-related genes (bap, OmpA, abaI, csuA/B, A1S_2091, A1S_1510, A1S_0690, A1S_0114) were estimated using QRT-PCR. In vitro infection model for analyzing the antibacterial activity of AgNPs was done using a co-culture infection model of A. baumannii with human fibroblast skin cell line HFF-1 or Vero cell lines. A. baumannii had high level of resistance to antibiotics. Most of the isolates harbored the fimH, afa/draBC, cnf1, csgA and cnf2, and the majority of A. baumannii produced strong biofilms. AgNPs inhibited the growth of A. baumannii efficiently with MIC ranging from 4 to 25 µg/ml. A. baumannii showed a reduced growth rate in the presence of AgNPs. The inhibitory activity and the anti-biofilm activity of AgNPs were more pronounced against the weak biofilm producers. Moreover, AgNPs decreased the expression of kpsMII , afa/draBC,bap, OmpA, and csuA/B genes. The in vitro infection model revealed a significant antibacterial activity of AgNPs against extracellular and intracellular A. baumannii. AgNPs highly interrupted bacterial multiplication and biofilm formation. AgNPs downregulated the transcription level of important virulence and biofilm-related genes. Our findings provide an additional step towards understanding the mechanisms by which sliver nanoparticles interfere with the microbial spread and persistence.

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Section: Introductionmentioning

confidence: 99%

Antibiofilm and antivirulence potential of silver nanoparticles against multidrug-resistant Acinetobacter baumannii

Hetta

Al-Kadmy

Khazaal

et al. 2021

Sci Rep

106

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“…In addition to the samples from indoor and built environment, soil samples from parks, JUST university campus and roundabouts were collected to investigate the presence of A. baumannii in an attempt to have an insight into its possible reservoir. Soil supplies micro‐organisms with basic needs, making it one of the best environments for them to survive and thrive (Adewoyin & Okoh, 2018). Few studies have demonstrated the isolation of A. baumannii from soils, especially polluted and contaminated ones (Byrne‐Bailey et al, 2009; Furlan et al, 2018; Hafa et al, 2019; Hrenovic et al, 2014; Obuekwe et al, 2009; Ranjbariyan et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Prevalence, genetic diversity, antibiotic resistance and biofilm formation of Acinetobacter baumannii isolated from urban environments

Ababneh

Laila

Jaradat

2022

Journal of Applied Microbiology

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Aim: Acinetobacter baumannii is a well-known nosocomial pathogen that has been isolated from different clinical sources. This pathogen also causes communityacquired infections, with mortality rates as high as 64%. The exact natural habitat of this bacterium is still unknown. In this study, we investigated the prevalence of A. baumannii in diverse soil and high-touch surface samples collected from a university campus, malls, parks, hypermarkets and produce markets, roundabout playground slides and bank ATMs. Methods and Results: All obtained isolates were characterized for their antibiotic susceptibility, biofilm formation capacities, and were typed by multi-locus sequence analysis. A total of 63 A. baumannii isolates were recovered, along with 46 Acinetobacter pittii and 8 Acinetobacter nosocomialis isolates. Sequence typing revealed that 25 A. baumannii isolates are novel strains. Toilets and sink washing basins were the most contaminated surfaces, accounting for almost 50% of the isolates. A number of A. baumannii (n = 10), A. pittii (n = 19) and A. nosocomialis (n = 5) isolates were recovered from handles of shopping carts and baskets. The majority of isolates were strong biofilm formers and 4 isolates exhibited a multi-drug resistant phenotype. Conclusions:Our study is the first to highlight community restrooms and shopping carts as potential reservoirs for pathogenic Acinetobacter species. Further studies are required to identify the reasons associated with the occurrence of A. baumannii inside restrooms. Proper disinfection of community environmental surfaces and spreading awareness about the importance of hand hygiene may prevent the dissemination of pathogenic bacteria within the community.Significance and Impact of the study: Serious gaps remain in our knowledge of how A. baumannii spreads to cause disease. This study will advance our understanding of how this pathogen spreads between healthcare and community environments.

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“…Enterobacteriaceae are a group of gram-negative bacteria with strong degradation ability for organic compounds, which dominate in many plant endophytic microflora and are the endophytic core microflora of many plant seeds; members of this family are related to plant health and/or disease ( Rossmann et al., 2012 ; Erlacher et al., 2015 ; Cernava et al., 2019 ; Zhang et al., 2020 ; Bziuk et al., 2021 ). In other relatively high-abundance core endo-bacteriome, Acinetobacter is a class of gram-negative bacteria, and some strains of this genus can grow hormones, solubilise phosphate, and produce siderophores, which can promote plant growth ( Sachdev et al., 2010 ; Rokhbakhsh-Zamin et al., 2011 ; Adewoyin and Okoh, 2018 ). In this study, Acinetobacter accounted for a relatively high proportion of Lhasa landraces and modern cultivars and was the most abundant bacterial genus in the seeds of modern cultivars.…”

Section: Discussionmentioning

confidence: 99%

Deciphering the core seed endo-bacteriome of the highland barley in Tibet plateau

Zhao¹,

Wang²,

Guo³

et al. 2022

Front. Plant Sci.

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Highland barley (Hordeum vulgare var. nudum (L.) Hook.f., qingke) has unique physical and chemical properties and good potential for industrial applications. As the only crop that can be grown at high altitudes of 4200–4500 m, qingke is well adapted to extreme habitats at high altitudes. In this study, we analysed the seed bacterial community of 58 genotypes of qingke grown in different regions of Tibet, including qingke landraces, modern cultivars, and winter barley varieties, and characterised endophytic bacterial communities in seeds from different sources and the core endo-bacteriome of qingke. This study aim to provide a reference for the application of seed endophytes as biological inoculants for sustainable agricultural production and for considering microbe-plant interactions in breeding strategies. A total of 174 qingke seed samples from five main agricultural regions in Tibet were collected and subjected to investigation of endophytic endo-bacteriome using high-throughput sequencing and bioinformatics approaches. The phyla of endophytic bacteria in qingke seeds from different sources were similar; however, the relative proportions of each phylum were different. Different environmental conditions, growth strategies, and modern breeding processes have significantly changed the community structure of endophytic bacteria in seeds, among which the growth strategy has a greater impact on the diversity of endophytic bacteria in seeds. Seeds from different sources have conserved beneficial core endo-bacteriome. The core endo-bacteriome of qingke seeds dominated by Enterobacteriaceae may maintain qingke growth by promoting plant growth and assisting plants in resisting pests and diseases. This study reveals the core endo-bacteriome of qingke seeds and provides a basis for exploiting the endophytic endo-bacteriome of qingke seeds.

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The natural environment as a reservoir of pathogenic and non-pathogenic Acinetobacter species

Cited by 48 publications

References 67 publications

Antibiofilm and antivirulence potential of silver nanoparticles against multidrug-resistant Acinetobacter baumannii

Antibiofilm and antivirulence potential of silver nanoparticles against multidrug-resistant Acinetobacter baumannii

Prevalence, genetic diversity, antibiotic resistance and biofilm formation of Acinetobacter baumannii isolated from urban environments

Deciphering the core seed endo-bacteriome of the highland barley in Tibet plateau

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