The natural product vioprolide A exerts anti-inflammatory actions through inhibition of its cellular target NOP14 and downregulation of importin-dependent NF-ĸB p65 nuclear translocation

Burgers, Luisa D.; Luong, Betty; Li, Yanfen; Fabritius, Matthias P.; Michalakis, Stylianos; Reichel, Christoph; Müller, Rolf; Fürst, Robert

doi:10.1016/j.biopha.2021.112255

Cited by 9 publications

(4 citation statements)

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“…These inflammatory circumstances comprise extensive immune cell activation and expansion, dilation of the dermal microvasculature, and, consequently, accelerated leukocyte recruitment to the injured tissue [ 46 ]. To evaluate whether S. pilosa influenced inflammatory processes through altering the leukocyte–endothelial cell interaction, a well-established cell-adhesion assay with TNF as the inflammatory stimulus was performed [ 47 , 48 ]. For this, a monolayer of endothelial cells was pretreated with the extracts and isolated compounds from S. pilosa for 30 min, and then was inflammatorily activated using TNF, followed by the adhesion of fluorescence-labeled THP-1 cells, a monocytic cell line ( Figure 6 ).…”

Section: Resultsmentioning

confidence: 99%

HPLC- and NMR-Based Chemical Profiling, Wound-Healing Potential, Anti-Inflammatory and Antibacterial Activities of Satureja pilosa (Lamiaceae), a Neglected Medicinal–Aromatic Herb

Panagiotidou,

Burgers,

Tsadila

et al. 2023

Plants

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Satureja pilosa Velen. (Lamiaceae) is a perennial and melliferous aromatic–medicinal subshrub which is range-restricted in adjacent parts of Greece and Bulgaria and locally in Italy, known in Northern Greece as wild oregano (“agriorigani”) and traditionally collected from the wild for culinary purposes. Since the ethnopharmacological data and modern biological activities of Satureja spp. suggest promising applications in skin conditions, the present study aimed to investigate the hitherto unknown phenolic content of cultivated S. pilosa and its potential biological activities, focusing mainly on wound-healing and anti-inflammatory effects. An HPLC-PDA-MS-targeted phytochemical investigation, along with NMR, allowed for the isolation and characterization of the main constituents, resulting in 18 compounds. Representative extracts and purified compounds were tested for wound-healing activity on NIH/3T3 fibroblasts. The butanol extract exhibited a significantly higher cell migration rate (73.4%) compared to aqueous (50.6%) and methanolic (49.6%) ones, enhancing the cell migration more rapidly at both concentration levels, whilst rosmarinic acid was the most potent among the isolated compounds, with a migration rate of 64.0% at the concentration level of 10−5 mg/mL, followed by 3,4-dihydrophenyllactic acid (54.7%). Moreover, potential effects on endothelial activation processes were explored, including the leukocyte–endothelial cell interaction during inflammatory processes and the migratory capacity during angiogenic actions, since these processes are commonly associated with skin diseases. Finally, extracts and purified compounds demonstrated weak antibacterial potential against two important pathogens (Staphylococcus aureus and Pseudomonas aeruginosa), suggesting that further investigation is warrented.

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Section: Resultsmentioning

confidence: 99%

HPLC- and NMR-Based Chemical Profiling, Wound-Healing Potential, Anti-Inflammatory and Antibacterial Activities of Satureja pilosa (Lamiaceae), a Neglected Medicinal–Aromatic Herb

Panagiotidou,

Burgers,

Tsadila

et al. 2023

Plants

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“…NOP14 , which is an evolutionally conserved protein in eukaryotes, mainly locates in nucleus and nucleus membrane and plays an important role in pre-18 s rRNA processing and small ribosomal subunit assembly [ 15 ]. It has been recently reported that NOP14 can be considered as an immune regulator factor, and that is involved in various diseases [ 16 , 17 ]. In chronic inflammatory disease, NOP14 activates NF-κB signaling pathway, and upregulates proinflammatory gene expression, and increases the expression of adhesion molecules in endothelial cells, thereby promoting the infiltrations of immune cells [ 18 ].…”

Section: Discussionmentioning

confidence: 99%

“…It has been recently reported that NOP14 can be considered as an immune regulator factor, and that is involved in various diseases [ 16 , 17 ]. In chronic inflammatory disease, NOP14 activates NF-κB signaling pathway, and upregulates proinflammatory gene expression, and increases the expression of adhesion molecules in endothelial cells, thereby promoting the infiltrations of immune cells [ 18 ]. This highlights that NOP14 is closely related with immune response and that it plays an important role in biological processes of various cells.…”

Section: Discussionmentioning

confidence: 99%

Increased expression of NOP14 is associated with improved prognosis due to immune regulation in colorectal cancer

Liao²,

Huang

et al. 2022

BMC Gastroenterol

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Background Colorectal cancer (CRC) is the third most common of cancer-related deaths. Nucleolar protein 14 (NOP14) is known to play different roles in diverse types of cancers. However, little is known about its roles in CRC. Here, we assessed the prognostic value and functions of NOP14 in CRC using the data from The Cancer Genome Atlas (TCGA) and validated them based on the data from Gene Expression Omnibus (GEO). Methods NOP14 mRNA and protein data in CRC was obtained from the TCGA, GEO, human protein atlas (HPA), and UALCAN databases. Survival and Cox regression analysis was performed to assess the prognostic value of NOP14 in CRC patients. Next, to evaluate the potential functions of NOP14, a protein–protein interaction (PPI) network was constructed and gene set enrichment analysis (GSEA) of differential expression genes (DEGs) associated with dysregulated NOP14 was performed. Finally, to investigate the immune response associated with NOP14 expression in CRC, we analyzed the correlations between immune cells infiltration and NOP14 expression level. Additionally, the correlations between immune molecule expression levels with NOP14 expression level were analyzed. Results High NOP14 mRNA expression was observed in CRC tissues based on the data from TCGA and GEO datasets. Similarly, high NOP14 protein levels were found in CRC tissues according to the immunohistochemical images from HPA. Interestingly, high NOP14 expression level was associated with an improved prognosis in CRC patients. Univariate and multivariate Cox regression analysis indicated that high NOP14 expression level was an independent protective factor for CRC patients. With the support of PPI network analysis, we found several risk genes interacted with NOP14. GSEA revealed that high NOP14 expression inhibited several signal pathways involved in tumor formation and development. Additionally, high NOP14 expression was positively associated with most kinds of immune cell infiltrations and the expression levels of some molecules related to immune activation. Conclusion Altogether, these results indicated that high NOP14 expression leads to improved prognosis in CRC patients by inhibiting the signaling pathways involved in tumor growth and promoting the immune responses.

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“…Human umbilical vein endothelial cells (HUVECs) were isolated from human umbilical veins, as previously described [ 22 , 23 ]. These HUVECs were cultivated to passage 2 and used for experimental purposes in passage 3.…”

Section: Methodsmentioning

confidence: 99%

Integrating Siderophore Substructures in Thiol-Based Metallo-β-Lactamase Inhibitors

et al. 2023

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Metallo beta lactamases (MBLs) are among the most problematic resistance mechanisms of multidrug-resistant Gram-negative pathogens due to their broad substrate spectrum and lack of approved inhibitors. In this study, we propose the integration of catechol substructures into the design of thiol-based MBL inhibitors, aiming at mimicking bacterial siderophores for the active uptake by the iron acquisition system of bacteria. We synthesised two catechol-containing MBL inhibitors, as well as their dimethoxy counterparts, and tested them for in vitro inhibitory activity against NDM-1, VIM-1, and IMP-7. We demonstrated that the most potent catechol-containing MBL inhibitor is able to bind Fe3+ ions. Finally, we could show that this compound restores the antibiotic activity of imipenem in NDM-1-expressing K. pneumoniae, while leaving HUVEC cells completely unaffected. Thus, siderophore-containing MBL inhibitors might be a valuable strategy to overcome bacterial MBL-mediated resistance to beta lactam antibiotics.

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The natural product vioprolide A exerts anti-inflammatory actions through inhibition of its cellular target NOP14 and downregulation of importin-dependent NF-ĸB p65 nuclear translocation

Cited by 9 publications

References 45 publications

HPLC- and NMR-Based Chemical Profiling, Wound-Healing Potential, Anti-Inflammatory and Antibacterial Activities of Satureja pilosa (Lamiaceae), a Neglected Medicinal–Aromatic Herb

HPLC- and NMR-Based Chemical Profiling, Wound-Healing Potential, Anti-Inflammatory and Antibacterial Activities of Satureja pilosa (Lamiaceae), a Neglected Medicinal–Aromatic Herb

Increased expression of NOP14 is associated with improved prognosis due to immune regulation in colorectal cancer

Integrating Siderophore Substructures in Thiol-Based Metallo-β-Lactamase Inhibitors

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