The Nature of Chemiluminescent Reactions

Abstract

Chemiluminescence has evolved from being an interested fact, via a promising analytical technique, to a fairly well understood phenomenon. The research during the last two decades has resulted in a much better understanding of the chemistry of chemiluminescence. This has lead to exciting (sic) new systems which are not only interesting from a basic scientific perspective, but also has resulted in commercial exploitation of chemiluminescence in all kinds of applications. This chapter surveys the most important chemiluminescent systems (for example, those involving luminol, dioxetanes, acridan esters, peroxyoxalates), and describes the properties of such chemiluminescent systems and the known or hypothesised molecular mechanisms involved in their chemiluminescence emission.

Dioxetane-Doped Silica Nanoparticles as Ultrasensitive Reagentless Thermochemiluminescent Labels for Bioanalytics

“…In fact, thermal decomposition of these compounds should lead to N-substituted acridanones in the singlet excited state, which have fluorescence quantum yields higher than that of adamantanone (Φ F = 0.015). Indeed, 1,2-dioxetanes containing an acridine moiety have been postulated as intermediates in the CL reaction of acridinium salts and acridane esters …”

Peptide probes for proteases – innovations and applications for monitoring proteolytic activity

“…The phenomena of chemiluminescence can be defined as the emission of light resulting from a chemical reaction (Scheme 6) and differs from fluorescence in that the generation of the excited state arises thanks to the energy of a chemical reaction and does not depend on light irradiation. 151 The field of chemiluminescence for imaging applications has grown rapidly with the discovery of several natural and new synthetic luminogens. 152,153…”

“…The phenomena of chemiluminescence can be defined as the emission of light resulting from a chemical reaction (Scheme 6) and differs from fluorescence in that the generation of the excited state arises thanks to the energy of a chemical reaction and does not depend on light irradiation. 151 The field of chemiluminescence for imaging applications has grown rapidly with the discovery of several natural and new synthetic luminogens. 152,153 Synthetic chemiluminescent systems take inspiration from natural bioluminescent systems that generate light following two sequential reactions, firstly oxidation of a substrate by an oxidizing agent or an enzyme into a ''high energy intermediate'' and secondly, the rapid decomposition of this intermediate in a process that produces light, with the Luciferin/Luciferase/O 2 system being the most studied.…”

Paper-Based Immunosensors with Bio-Chemiluminescence Detection

“…HRP shows good stability combined with a high enzymatic turnover number, making it an excellent candidate for CL-based biospecific assays. By adding to the CL cocktails suitable enhancers acting as electron transfer mediators (e.g., substituted phenols, substituted boronic acids, indophenols, N-alkyl phenothiazines [ 17 ] and 4-dialkylaminopyridine [ 18 ]), it is possible to increase the signal intensity and to stabilize the CL signal over time.…”