The nature of pre beta (very low density) lipoproteins.

Levy, Robert I.; Lees, Rachel; Fredrickson, Donald S.

doi:10.1172/jci105324

Cited by 194 publications

(49 citation statements)

References 31 publications

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“…Discussion Both absence and deficiency of beta lipoproteins have been described in patients with abetalipoproteinemia (2-5, 9, 11, 30, 31 (13,32), but more complete delipidation usually denatures the protein residue (13,32,33), making immunochemical analyses quite difficult. Proof is therefore lacking that apoprotein B would always be recognized by antisera that react with its lipid-laden form.…”

Section: Methodsmentioning

confidence: 99%

“…Lipoprotein fractions were isolated in the preparative ultracentrifuge by techniques identical to those previously described (12 hours to permit isolation of any very low density lipoprotein (VLDL) (13). Isotonic NaCl solution was usually added to the top and bottom fractions to return them to plasma concentrations before they were further utilized.…”

Section: Methodsmentioning

confidence: 99%

“…The paper strips were dried in an oven at 1000 C for 30 min-1 Sample kindly provided by Dr Immunochemistry. The techniques employed for immunoelectrophoresis and double diffusion on plates or in microtubes with either agar or agarose were previously described (12,13). The preparation and characteristics of the specific antisera to alpha or beta lipoproteins and the antiwhole human sera that were used were described earlier (12).…”

Section: Methodsmentioning

confidence: 99%

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The lipoproteins and lipid transport in abetalipoproteinemia.

Levy¹,

Fredrickson²,

Laster³

1966

J. Clin. Invest.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

The lipoproteins and lipid transport in abetalipoproteinemia.

Levy¹,

Fredrickson²,

Laster³

1966

J. Clin. Invest.

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“…The infranate was then adjusted to a density of 1.063 g/ml by the addition of NaCl-KBr solution of density 1.35 g/ml and subjected to further ultracentrifuga- Each preparation of LDL used in turnover studies was demonstrated to be free of contaminating lipoproteins by immunoelectrophoresis in agarose gel employing specific antisera prepared against high density (alpha), low density (beta) lipoproteins, and very low density lipoprotein apoproteins (4). In addition, no other contaminating serum proteins were found using antisera reacting with albumin, gamma globulin, and whole human serum (4).…”

Section: Preparation Of Ldlmentioning

confidence: 96%

The metabolism of low density lipoprotein in familial type II hyperlipoproteinemia

Langer¹,

Strober²,

Levy³

1972

J. Clin. Invest.

617

182

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A B S T R A C T The metabolism of low density lipoprotein (LDL, beta lipoprotein) was studied in 10 normal individuals and 10 patients with familial type II hyperlipoproteinemia using purified radioiodinated LDL. Over 97% of the label was bound to the protein moiety of LDL and therefore the turnover data reflect the fate and distribution of LDL-apoprotein. Comparison of the metabolic behavior of biologically screened and utnscreened labeled LDL preparations in dogs as well as the analysis of the urinary excretion of radioiodide derived from labeled LDL degradation in humans indicated that no significant denaturation resulted from the isolation, purification, and labeling techniques.The plasma concentration of LDL-cholesterol in nor-.mals was 105±21 mg/100 ml (mean +1 SD) in contrast to 254±47 mg/100 mg in patients with type II hyperlipoproteinemia; these values corresponded to LDL-apoprotein concentrations of 63±13 mg/100 ml and 153±(30 mg/100 ml, respectively. Despite these differences in concentration, the synthetic rate of LDL-apoprotein in both groups was not significantly different (14

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“…Plates were developed at 4°C and were observed for 7 days. The immunoelectrophoretic techniques used have been previously described (31,32). Rabbit antisera to LDL or apoLDL were produced by two injections into the foot pads of white New Zealand rabbits of 1.25 ml of protein solution (6-8 mg of protein) mixed with 0.75 ml of complete Freund's adjuvant.…”

Section: Immunological Techniquesmentioning

confidence: 99%

Evidence for the identity of the major apoprotein in low density and very low density lipoproteins in normal subjects and patients with familial hyperlipoproteinemia

Gotto¹,

Brown²,

Levy³

et al. 1972

J. Clin. Invest.

108

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A B S T R A C T The major apoprotein (s) from human plasma low density lipoproteins was isolated and compared with a major protein fraction (fraction I) from very low density lipoproteins (VLDL). Fraction I had been previously found to comprise approximately 40% of the total protein of VLDL. Fraction I from VLDL and apoLDL from normal subjects were indistinguishable in amino acid compositions and circular dichroic spectra. They yielded indistinguishable displacement curves of LDL-'I by radioimmunoassay and formed immunoprecipitin lines of complete identity. Fraction I from VLDL of normal subjects was compared with the fraction isolated from patients with familial types II, III, IV, and V hyperlipoproteinemia. There were no detectable differences between any of these fractions in amino acid compositions, circular dichroic spectra, and immunochemical properties. It was, therefore, concluded that short of peptide mapping or determination of amino acid sequences, fraction I from VLDL of each subject with familial hyperlipoproteinemia appears to be identical with fraction I and apoLDL from normal individuals.A new convenient method of preparation of soluble apoLDL, modified from a procedure previously described from this laboratory, is presented.

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The nature of pre beta (very low density) lipoproteins.

Cited by 194 publications

References 31 publications

The lipoproteins and lipid transport in abetalipoproteinemia.

The lipoproteins and lipid transport in abetalipoproteinemia.

The metabolism of low density lipoprotein in familial type II hyperlipoproteinemia

Evidence for the identity of the major apoprotein in low density and very low density lipoproteins in normal subjects and patients with familial hyperlipoproteinemia

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