The Nature of the Membrane Injury in Irradiated Human Erythrocytes

Shapiro, Barry A.; Kollmann, George

doi:10.2307/3572559

Cited by 58 publications

(11 citation statements)

References 9 publications

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“…4 Effect of CPZ on 16 O-induced microsomal (0.6 mg/ml) lipid peroxidation molysis increased with time, the rate varied as a function of fluence. The hemolytic effect could be ascribed to disturbances in the membrane electrolytic transport [28]. It was quite possible that the free radicals, particularly · OH, apart from inducing the cross-linking in the membranes had oxidised the sulphydryl groups [9,29].…”

Section: Resultsmentioning

confidence: 99%

Effect of high linear energy transfer radiation on biological membranes

Choudhary

Srivastava

Sarma

et al. 1998

Radiation and Environmental Biophysics

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Cellular membranes are vital elements, and their integrity is extremely essential for the viability of the cells. We studied the effects of high linear energy transfer (LET) radiation on the membranes. Rabbit erythrocytes (1 x 10(7) cells/ml) and microsomes (0.6 mg protein/ml) prepared from liver of rats were irradiated with 7Li ions of energy 6.42 MeV/u and 16O ions of energy 4.25 MeV/u having maximum LET values of 354 keV/microm and 1130 keV/microm, respectively. 7Li- and 16O-induced microsomal lipid peroxidation was found to increase with fluence. The 16O ions were more effective than 7Li ions, which could be due to the denser energy distribution in the track and the yield of free radicals. These findings suggested that the biological membranes could be peroxidized on exposure to high-LET radiation. Inhibition of the lipid peroxidation was observed in the presence of a membrane-active drug, chlorpromazine (CPZ), which could be due to scavenging of free radicals (mainly HO* and ROO*), electron donation, and hydrogen transfer reactions. The 7Li and 16O ions also induced hemolysis in erythrocytes. The extent of hemolysis was found to be a function of time and fluence, and showed a characteristic sigmoidal pattern. The 16O ions were more effective in the lower fluence range than 7Li ions. These results were compared with lipid peroxidation and hemolysis induced by gamma-radiation.

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Section: Resultsmentioning

confidence: 99%

Effect of high linear energy transfer radiation on biological membranes

Choudhary

Srivastava

Sarma

et al. 1998

Radiation and Environmental Biophysics

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“…27,28 Radiation mainly involves sulfhydryl groups which produces damage to human erythrocyte membrane. 29,30 Such damage occurs by a site specific formation of hydroxyl radicals by transition metal ions bound to proteins. 31 It is shown that that senescent cell antigens present in old and damaged erythrocytes have been derived from band 3 protein and these antigens play a role in removal of aged red blood cells from circulation.…”

Section: Discussionmentioning

confidence: 99%

Degradation of Spectrin and band 3 protein

Audi¹

2020

IJCBR

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Oxygen free radicals generated during radiation injury damage human erythrocyte membrane proteins including spectrin and band 3 protein. Results show an appreciable degree of spectrin and band 3 protein loss of about 55-65% due to crosslinking, and proteolysis when sodium dodecyl sulphate polyacrylamide gel electrophoresis carried out on erythrocyte ghosts. Oxidative damage to membrane proteins lead to degradation of its cytoskeletal components.

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“…The importance of sulfhydryls and unsaturated fatty acids have been shown in those membrane alterations induced by irradiation (33,36). In agreement with this, EPM reduction after irradiation has been shown to be inhibited by sulfhydryl blocking agents and by low temperature (25).…”

Section: Discussionmentioning

confidence: 99%

No elevated cell surface charge at mitosis in X-ray-irradiated mammalian cells.

Nishizawa

Kojima

Sato

1985

Cell Struct. Funct.

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ABSTRACT. Rounded mitotic cells showed 30 % enhanced electrophoretic mobility (EPM) when compared to spindle-formed interphase cells. This increase in EPM that was not present in interphase cells that had been rounded chemically by EDTA is considered to reflect a structural change in the cell membrane during mitosis. X-ray irradiation induced a dose-dependent EPM decrease in both interphase and mitotic cells during a 4-hour period. During the next 20 h of incubation, EPM recovery took place in cells irradiated with 250R, but not in cells exposed to 1000R. EPM was enhanced during mitosis in cells irradiated with low doses, but was absent in cells irradiated with 1000R. The ratio of colony-forming cells and of electrophoretically recovered mitotic cells after 24 h of exposure showed a good statistical correlation. These results indicate that unrepaired membrane damage contributes to mitotic cell death after irradiation.We used an electrophoretic assay to investigate membrane damage to cells that showed impaired proliferation and death after X-ray irradiation. The advantages of our method are its high sensitivity as a probe for detecting alterations in the dynamic physiological charge-related properties on the surface of living cells (6, 11, 15) and the simultaneous detection of electrophoretic mobility (EPM) and the morphology of individual cells under phase microscopy.Decreases in EPM after irradiation have been reported in several types of mammalian cells and in yeasts (19, 20, 25). Our previous studies of three lines of cultured mammalian cells showed that the percent of cells whose EPM did not recover within 24 h after irradiation agreed statistically with the ratio of non-colony forming cells (23, 24,26). Our first studies of the relation between cell division and the cell surface charge on individual cells used Escherichia coli as the model system. E. coli, which is inhibited during cell division, is visible during EPM measurements because of its filamentous form. The results for E. coli indicated that EPM decreased in cells whose division was inhibited and that recovery took place when cell division was resumed (30).Since Spear and Glticksmann's 1938 report, the common belief has been that cellular death is associated with mitosis after X-ray irradiation. Irradiation does not result in immediate death, it is delayed until the first mitosis after irradiation (40).

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The Nature of the Membrane Injury in Irradiated Human Erythrocytes

Cited by 58 publications

References 9 publications

Effect of high linear energy transfer radiation on biological membranes

Effect of high linear energy transfer radiation on biological membranes

Degradation of Spectrin and band 3 protein

No elevated cell surface charge at mitosis in X-ray-irradiated mammalian cells.

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