The nature of the serum alkaline phosphatases in liver diseases

Price, Christopher P.; Sammons, H. G.

doi:10.1136/jcp.27.5.392

Cited by 54 publications

(35 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, the profound elevations in the serum AST and ALP in the CFA-treated rats could also have arisen from extra-hepatic sources such as acute myocardial infarction and/or bone diseases and remodeling (Kantharia and Woolf, 1987;Aida, 1993). Thus, the profound elevation in the serum ALP in the arthritic rats strongly points to the establishment of an active bone lesion such as arthritis in the CFA-treated rats which is in complete agreement with results of other studies (Price and Sammons, 1974;Rosalki et al, 1982;. Further elevations in the serum levels of AST and ALP caused by oral treatment with 100-400 mg/kg of HEAC in both pre-and post-arthritic rat models could possibly have arisen from worsening of the chemically induced arthritis or remodeling phase of bone healing.…”

Section: Discussionsupporting

confidence: 81%

Evaluation Of The Anti-Arthritic Activity Of The Hydroethanolic Leaf Extract Of <i>Alchornea Cordifolia</i> In Rats

Adeneye¹,

Oreagba²,

Ishola³

et al. 2014

Afr. J. Trad. Compl. Alt. Med.

View full text Add to dashboard Cite

Background: Different decoctions of Alchornea cordifolia leaves are used by Yoruba herbalists (Southwest Nigeria) for the local treatment of ulcers, rheumatic pains, febrile convulsions, and for enhancing physical performance. Materials and methods: In this study, the anti-arthritic effect of 100 -400 mg/kg/day of the hydroethanolic leaf extract of Alchornea cordifolia (HEAC) was investigated in Complete Freund's Adjuvant (CFA)-induced arthritic rats as a way of evaluating its efficacy in the local management of arthritis. In addition, the effects of HEAC on liver and renal function parameters as well as its effect on the antioxidant enzyme system were investigated. Arthritis was induced using 0.1 ml of 10 mg/ml of Complete Freund's Adjuvant (CFA) following 1 h oral pretreatment and 8 th day postarthritic induction with 100, 200 and 400 mg/kg/day of HEAC and 3 mg/kg/day of celecoxib as the reference drug. The anti-arthritic activity of HEAC was assessed based on the ability of HEAC to alter the paw edema diameter, body weight, full blood count, renal and liver function markers, glycoprotein, lysosomal enzymes and possible antioxidant potential in the arthritic rats. Results: Oral pretreatment with 100, 200, and 400 mg/kg/day of HEAC produced significant (p<0.001, p<0.05 and p<0.01) reductions in the paw edema diameter in a non-dose dependent fashion in ACF-induced arthritic rats with the 100 mg/kg/day of HEAC producing the most significant antiarthritic effect. Similarly, HEAC increased hepatic GSH levels, CAT and SOD activities suggesting possible antioxidant mechanism for its antiarthritic effect. Conclusion: Overall, results of this study lend credence to the folkloric use of water decoction of Alchornea cordifolia leaves against rheumatoid arthritis. However, further pharmacological investigations would be required at isolating and determining the active anti-arthritic molecule(s) in HEAC in the nearest future.

show abstract

Section: Discussionsupporting

confidence: 81%

Evaluation Of The Anti-Arthritic Activity Of The Hydroethanolic Leaf Extract Of <i>Alchornea Cordifolia</i> In Rats

Adeneye¹,

Oreagba²,

Ishola³

et al. 2014

Afr. J. Trad. Compl. Alt. Med.

View full text Add to dashboard Cite

show abstract

“…Although all enzyme biomarkers of cholestasis are membrane bound, the majority of serum cholephilic enzyme activity is believed to be normally hydrophilic (Price and Sammons, 1974;De Broe et al, 1975;Tsuji et al, 1980). While this could imply the production of specialized secretory forms of these enzymes, it has actually been shown that the enzymes are synthesized in their normal form but then become solubilized through the loss of their hydrophobic membrane anchoring motifs Hoffmann, 1995, 1999).…”

Section: Cellular Redistribution Of Cholephilic Enzyme Activitymentioning

confidence: 99%

Clinical Pathology Approaches to Hepatic Injury

Solter

2005

Toxicol Pathol

View full text Add to dashboard Cite

Testing the blood for evidence of hepatic damage and dysfunction frequently involves measuring several blood constituents simultaneously to screen for disease. While useful, this approach occasionally leads to apparent disparities between the blood test results, and the results of other diagnostic tests such as histology. In part, these perceived discrepancies may stem from a lack of appreciation for tissue, cellular, and molecular factors that affect the appearance of hepatic disease biomarkers in the blood. Further confusing the matter is that in some instances the mechanisms responsible for the appearance of diagnostic compounds in blood are only partially understood. Many of the known factors that affect hepatic biomarkers are similar to those affecting other tissue markers, while others are unique to the liver, such as those involved with cholestasis. Disease conditions can also cause misleading results by affecting tissue concentrations of test compounds, hepatic mass, and the clearance rate of compounds from the blood. Knowledge of the factors affecting the blood concentrations of biomarkers, as well as investigations into the mechanisms behind changes to hepatic biomarker concentrations, may allow for a better interpretation of blood test results and fewer inconsistencies between diagnostic results.

show abstract

“…Gombos (1976) also described protein denaturation by organic solvents in extraction of membranes. However, studies using other enzymes have shown that they may be delipidated by organic solvents and that activity may either be retained (Price and Sammons, 1974), or reactivation may take place by readdition of phospholipid (Vore et al, 1974;IngelmanSundberg, 1977). It is not yet known whether the loss of y-GT activity which occurs with butanol is caused by denaturation or by delipidation, but Discussion to butanol: 88 % had more than 75 % residual activity, and most patients retained 100% of y-GT activity.…”

Section: Group 4 Reflected Normal Sera In Their Responsementioning

confidence: 99%

Butanol Extraction of Serum and Urinary Gamma-Glutamyltransferase and its Application in Clinical Diagnosis

Beck

1978

Ann Clin Biochem

View full text Add to dashboard Cite

Summary Gamma-glutamyltransferase (γ-GT) activity was measured in serum and urine before and after extraction with n-butanol. Residual γ-GT activity after extraction of normal serum with butanol was more than 75 %, whereas for normal urine the figure was less than 68 %. The loss of activity from urine was not affected by prior dialysis, and the stabilisation which resulted from adding bovine serum albumin at a concentration of 40 g/l did not approach the residual activity with normal serum. In patients with a variety of renal diseases, residual γ-GT activity in the urine after butanol extraction was inversely correlated with the creatinine clearance. Butanol extraction was performed on serum samples from 182 patients with a variety of diseases. Eighty-one per cent of patients with elevated γ-GT activities caused by hepatobiliary disease had an increased loss of activity after butanol extraction. By contrast, only 34 % of patients with increased γ-GT activities in whom there was no clinical or other biochemical evidence of hepatic disease, had increased loss of γ-GT activity after butanol. The reasons for differences between urinary and serum γ-GT in response to butanol, and the implications in interpreting serum γ-GT activities are discussed.

show abstract

The nature of the serum alkaline phosphatases in liver diseases

Cited by 54 publications

References 20 publications

Evaluation Of The Anti-Arthritic Activity Of The Hydroethanolic Leaf Extract Of <i>Alchornea Cordifolia</i> In Rats

Evaluation Of The Anti-Arthritic Activity Of The Hydroethanolic Leaf Extract Of <i>Alchornea Cordifolia</i> In Rats

Clinical Pathology Approaches to Hepatic Injury

Butanol Extraction of Serum and Urinary Gamma-Glutamyltransferase and its Application in Clinical Diagnosis

Contact Info

Product

Resources

About