The NAV2 homolog Sickie regulates F-actin-mediated axonal growth in <i>Drosophila</i> mushroom body neurons via the non-canonical Rac-Cofilin pathway

Abe, Takashi; Murakami, Satoshi; Hiroi, Makoto; Nitta, Yohei; Maeyama, Yuko; Tabata, Tetsuya

doi:10.1242/dev.122713

Cited by 12 publications

(12 citation statements)

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“…Cofilin phosphorylation increases in the neural cells of sickie mutants and induces actin over-polymerization, resulting in the inhibition of the axonal outgrowth. Furthermore, the defect caused by the sickie mutation is rescued by overexpression of the active forms of cofilin or slingshot, but not their inactive forms (Abe et al 2014). These results indicate that axonal outgrowth requires cofilin dephosphorylation by the Rac-Sickie-slingshot pathway, which functions in parallel to the Rac-Pak-LIMK pathway.…”

Section: Neural Tissue Development and Neurite Growthmentioning

confidence: 84%

“…Studies have described the molecular mechanism by which cofilin phosphoregulation regulates axon growth. Loss of sickie, a Drosophila ortholog of the mammalian microtubuleassociated protein neuron navigator 2 (NAV2 MAP), suppresses axon growth of neural cells in the mushroom body (Abe et al 2014). Sickie, which is a Rac effector protein, is required for decreasing of the phosphorylation level of cofilin in developing neurons via a mechanism that depends on Rac but not on Pak.…”

Section: Neural Tissue Development and Neurite Growthmentioning

confidence: 99%

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Roles of cofilin in development and its mechanisms of regulation

Ohashi

2015

Dev Growth Differ

104

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Reorganization of the actin cytoskeleton is essential for cellular processes during animal development. Cofilin and actin depolymerizing factor (ADF) are potent actin-binding proteins that sever and depolymerize actin filaments, acting to generate the dynamics of the actin cytoskeleton. The activity of cofilin is spatially and temporally regulated by a variety of intracellular molecular mechanisms. Cofilin is regulated by cofilin binding molecules, is phosphorylated at Ser-3 (inactivation) by LIM-kinases (LIMKs) and testicular protein kinases (TESKs), and is dephosphorylated (reactivation) by slingshot protein phosphatases (SSHs). Although studies of the molecular mechanisms of cofilin-induced reorganization of the actin cytoskeleton have been ongoing for decades, the multicellular functions of cofilin and its regulation in development are just becoming apparent. This review describes the molecular mechanisms of generating actin dynamics by cofilin and the intracellular signaling pathways for regulating cofilin activity. Furthermore, recent findings of the roles of cofilin in the development of several tissues and organs, especially neural tissues and cells, in model animals are described. Recent developmental studies have indicated that cofilin and its regulatory mechanisms are involved in cellular proliferation and migration, the establishment of cellular polarity, and the dynamic regulation of organ morphology.

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Section: Neural Tissue Development and Neurite Growthmentioning

confidence: 84%

Section: Neural Tissue Development and Neurite Growthmentioning

confidence: 99%

Roles of cofilin in development and its mechanisms of regulation

Ohashi

2015

Dev Growth Differ

104

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“…Cofilin is also a substrate of the PAK1 kinase, which is required for neuronal polarity in culture (Jacobs et al, 2007). In the Drosophila mushroom body, two antagonistic signaling pathways downstream of Rac converge onto Cofilin to regulate axon growth (Abe et al, 2014). Rac1 signaling also controls polarity and axonal growth by recruiting the actin-regulatory complex WAVE1 to the membrane (Tahirovic et al, 2010).…”

Section: Actin Destabilization In Polarity and Growthmentioning

confidence: 99%

Establishing Neuronal Polarity with Environmental and Intrinsic Mechanisms

Yogev

Shen

2017

Neuron

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Neurons are among the most morphologically complex cells. A distinction between two compartments, axon and dendrite, generates cellular domains that differ in membrane composition and cytoskeletal structure, and sets the platform on which morphogens, transcription programs, and synaptic activity sculpt neuronal form. The establishment of this distinction, called Neuronal Polarity, entails interpreting spatial and intrinsic cues and converting them to cytoskeletal rearrangements that give rise to axons and dendrites. Hence, this early developmental event underpins the future functional properties of the neuron to receive and transmit information. Here we review the current understanding of developmental cues and cell biological mechanisms that establish polarity in newborn neurons, synthesizing information from vertebrate and invertebrate model systems.

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“…Histological analyses of fly brains were performed using a standard protocol (Abe et al, 2014;Huang and Kunes, 1996). After anesthetizing flies in ice-cold ethanol, brains were dissected in PBS and fixed for 30 min in 4% paraformaldehyde in PBS at room temperature.…”

Section: Histologymentioning

confidence: 99%

Two Parallel Pathways Assign Opposing Odor Valences during Drosophila Memory Formation

et al. 2018

Self Cite

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During olfactory associative learning in Drosophila, odors activate specific subsets of intrinsic mushroom body (MB) neurons. Coincident exposure to either rewards or punishments is thought to activate extrinsic dopaminergic neurons, which modulate synaptic connections between odor-encoding MB neurons and MB output neurons to alter behaviors. However, here we identify two classes of intrinsic MB γ neurons based on cAMP response element (CRE)-dependent expression, γCRE-p and γCRE-n, which encode aversive and appetitive valences. γCRE-p and γCRE-n neurons act antagonistically to maintain neutral valences for neutral odors. Activation or inhibition of either cell type upsets this balance, toggling odor preferences to either positive or negative values. The mushroom body output neurons, MBON-γ5β'2a/β'2mp and MBON-γ2α'1, mediate the actions of γCRE-p and γCRE-n neurons. Our data indicate that MB neurons encode valence information, as well as odor information, and this information is integrated through a process involving MBONs to regulate learning and memory.

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The NAV2 homolog Sickie regulates F-actin-mediated axonal growth in Drosophila mushroom body neurons via the non-canonical Rac-Cofilin pathway

Cited by 12 publications

References 0 publications

Roles of cofilin in development and its mechanisms of regulation

Roles of cofilin in development and its mechanisms of regulation

Establishing Neuronal Polarity with Environmental and Intrinsic Mechanisms

Two Parallel Pathways Assign Opposing Odor Valences during Drosophila Memory Formation

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