The Negative Effect of Repeated Equine Chorionic Gonadotropin Treatment on Subsequent Fertility in Alpine Goats Is Due to a Humoral Immune Response Involving the Major Histocompatibility Complex1

F, Roy; Maurel, M.C.; Combes, Bruno; Vaiman, Daniel; Cribiu, Edmond; Lantier, Isabelle; Pobel, T.; Deletang, F.; Combarnous, Yves; Guillou, Florian

doi:10.1095/biolreprod60.4.805

Cited by 91 publications

(77 citation statements)

References 33 publications

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“…Indeed, in the present experiment, only 15% and 39% of the does receiving 8 or 25 IU, respectively, developed a significant immune reaction just before the 11th injection. Recently, Roy et al (1999) demonstrated that the negative effect of repeated eCG treatments on subsequent fertility in Alpine goats was due to a humoral immune response involving the major histocompatibility complex. Unfortunately, we could not find any study on genetic sensibility to eCG realized in the rabbit species.…”

Section: Discussionmentioning

confidence: 99%

Evolution of anti-eCG antibodies in response to eCG doses and number of injections. Relationship with productivity of rabbit does

Theau-Clément¹,

Lebas²,

Beckers

et al. 2008

Animal

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The aim of this experiment was to study the kinetics of anti-eCG (equine chorionic gonadotrophin) antibodies in relation to eCG dose (8 or 25 IU) and number of injections (n 5 11) in comparison with a control group (no injection), and to relate antibody production to sexual receptivity and productivity of rabbit does. In all, 124 lactating primiparous rabbit does were inseminated every 35 days for a year. Just before eCG injection (48 h before insemination), blood samples were collected from all the does to assay anti-eCG antibodies. The anti-eCG antibody binding rate, regardless of the injected dose, shows that none of the does developed detectable anti-eCG antibodies before the 7th injection. The level of detectable anti-eCG antibodies began to show an increase at the 7th injection and was significant only for the 25 IU dose at the 11th injection. At the end of the experiment, 15% and 39% of does treated with 8 and 25 IU, respectively, developed immunity to eCG (binding rate .6%: higher binding rate of the control group). Consequently, the immune response depends on the eCG dose and on the number of injections. Moreover, productivity of does estimated from the number of weaned rabbits produced per insemination is not influenced by the level of eCG antibodies (7.0 and 6.9 for binding rate ,6% and binding rate >6%, respectively). Only 19 inseminations (n 5 6 and n 5 13 for 8 and 25 IU, respectively) were made on hyperimmune does. Consequently, the immune response to eCG seems to be marginal for rabbit does. Moreover, under the described experimental conditions, reproductive performances of hyperimmune does were not affected.

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Section: Discussionmentioning

confidence: 99%

Evolution of anti-eCG antibodies in response to eCG doses and number of injections. Relationship with productivity of rabbit does

Theau-Clément¹,

Lebas²,

Beckers

et al. 2008

Animal

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“…eCG's effect after repetitive use (revised by DRION et al, 1998;ROY et al, 1999a;DRION et al, 2001). the positive effect of eCG on follicle development has not been completely determined.…”

Section: Discussionmentioning

confidence: 99%

“…the eCG administration can increase the concentration of anti-eCG antibodies after the first use (ROY et al, 1999b;MaNtOVaNI, 2010) and some studies have reported an additive effect of subsequent treatments (ROY et al, 1999a;DRION et al, 2001). Furthermore, most studies have concluded that there is an animal individuality to the immune response against eCG (DRION et al, 2001;MaNtOVaNI, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…the production of anti-eCG antibodies has been reported in goats, sheep, cows, and rabbits (ROY et al, 1999a;ROY et al, 1999b;DRION et al, 2001;thEaU-ClEMENt et al, 2008, respectively). these antibodies are responsible for reducing the interaction of eCG with FSh and lh receptors (MaUREl et al, 2003).…”

Section: Introductionmentioning

confidence: 96%

“…as a consequence a delay could occur in the lh surge and ovulation, reducing the females' fertility. Some studies report antibody production from the first eCG application while others claim that there is an additive effect in repeated applications (ROY et al, 1999a;DRION et al, 2001;MaNtOVaNI, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Consequences of eight consecutive applications of equine chorionic gonadotropin on ovarian follicle development and ovulation in bos indicus cows

et al. 2016

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Ovarian and immunological responses to alternating exogenous gonadotropin regimens in the ocelot (Leopardus pardalis) and tigrina (Leopardus tigrinus)

et al. 2005

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Exogenous gonadotropins frequently are used to stimulate ovarian follicular growth and ovulation in mammalian species, including felids. However, repeated exogenous gonadotropin treatment can result in decreased ovarian responsiveness due to antibody formation. In this study, our objectives were to assess the effectiveness of alternating gonadotropin regimens on ovarian responses in ocelots and tigrinas, and investigate the humoral immune responses to these gonadotropins in each species. Females were treated four to six times with alternating equine chorionic gonadotropin (eCG)/human chorionic gonadotropin (hCG) and porcine follicle stimulating hormone (pFSH)/luteinizing hormone (pLH) regimens at 4-month intervals. With each treatment, the females were evaluated laparoscopically to assess ovarian follicular development and recover oocytes from mature follicles. Blood was collected before each treatment and at laparoscopy. Overall, the ocelots averaged more (Po0.05) follicles and corpus luteum (CL) (6.870.8; mean7SEM) per stimulation than the tigrinas (2.370.4), but the percentage of mature oocytes (mean range ¼ 54-55%) did not differ (Po0.05). Within species, both gonadotropin regimens were equally effective (P40.05) in inducing follicular growth and oocyte maturation. The total number of ovarian structures and oocyte maturation percentages did not decrease (Po0.05) in either species with sequential stimulations. Although the percentage of blood samples containing anti-gonadotropin immunoglobulins increased (Po0.05) with sequential treatment, the presence of positive titers did not cause a decrease (Po0.05) in ovarian responsiveness. In summary, the female ocelots and tigrinas continued to respond to these alternating ovarian stimulation protocols after repeated use, despite the formation of anti-gonadotropin antibodies in some of the females. These findings suggest that the use of alternating gonadotropin regimens may permit more intensive reproductive management of these endangered cat species for conservation. Zoo Biol 24: 247-260, 2005.

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The Negative Effect of Repeated Equine Chorionic Gonadotropin Treatment on Subsequent Fertility in Alpine Goats Is Due to a Humoral Immune Response Involving the Major Histocompatibility Complex1

Cited by 91 publications

References 33 publications

Evolution of anti-eCG antibodies in response to eCG doses and number of injections. Relationship with productivity of rabbit does

Evolution of anti-eCG antibodies in response to eCG doses and number of injections. Relationship with productivity of rabbit does

Consequences of eight consecutive applications of equine chorionic gonadotropin on ovarian follicle development and ovulation in bos indicus cows

Ovarian and immunological responses to alternating exogenous gonadotropin regimens in the ocelot (Leopardus pardalis) and tigrina (Leopardus tigrinus)

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