Recent large helical device experiments revealed that the transition from ion root to electron root occurred for the first time in neutral-beam-heated discharges, where no nonthermal electrons exist. The measured values of the radial electric field were found to be in qualitative agreement with those estimated by neoclassical theory. A clear reduction of ion thermal diffusivity was observed after the mode transition from ion root to electron root as predicted by neoclassical theory when the neoclassical ion loss is more dominant than the anomalous ion loss. Neoclassical ion transport is important in stellarator plasmas, because the helical ripple losses are comparable to or sometimes even higher than the anomalous losses in contrast to those in tokamaks. The crucial issues of neoclassical ion transport are (1) the reduction of ion thermal diffusivity due to a large positive radial electric field in the electron root [1][2][3], and (2) the reduction of ion thermal diffusivity due to the optimization of the magnetic field structure (s optimization) [3][4][5][6]. However, there has been no experimental study to test these issues on the neoclassical ion transport in stellarator plasmas. This is because the transition of the radial electric field from small negative (ion root) to large positive (electron root) was observed only in plasmas with the assistance of electron cyclotron heating (ECH), where electron heating is dominant [7][8][9][10][11]. The ion temperature is much lower than the electron temperature because ions are heated only by the energy exchange between ions and electrons. In these experiments, the significant increase of electron temperature and a clear reduction of electron thermal diffusivity were observed in the plasma core in the electron root. However, no reduction of ion thermal diffusivity was observed because of the lack of direct ion heating. There have been no experimental results to show the improvement of ion transport in the electron root, although a significant improvement of ion transport (rather than the electron transport) is predicted by the neoclassical theory [6]. This paper describes the experimental results of the neoclassical feature of ion transport for the first time, the reduction of ion thermal diffusivity due to the transition to the large positive electric field (electron root), and/or the optimization of the magnetic field structure (s optimization).The large helical device (LHD) [12] is a Heliotron device (poloidal period number L 2, and toroidal period number M 10) with a major radius of R ax 3.5 4.1 m, an average minor radius of 0.6 m, and magnetic field up to 3 T. The radial electric field ͑E r ͒ is derived from the poloidal and toroidal rotation velocity and pressure gradient of neon impurity measured with charge exchange spectroscopy [13] at the midplane in LHD (vertically elongated cross section) using a radial force balance. The radial force balance equation can be expressed as E r ͑en I Z I ͒ 21 ͑≠p I ͞≠r͒ 2 ͑y u B f 2 y f B u ͒, where B f and B u are toroidal a...