The Nephroprotective And Antioxidant Activity of Sterculia rubiginosa Zoll. Ex Miq. Leaves

Abstract

Background: Sterculia has an antioxidant activity. The Sterculia genus has phenols and flavonoids content, and this chemical content may be have an nephroprotective activity. Objective: The study was to investigate the in vitro study of antioxidant activity with DPPH and FRAP study and nephroprotective activity of Sterculia rubiginosa Zoll. Ex Miq. Leaves extract. Materials and Methods: The leaves was extracted using ethanol. This extract was determined for antioxidant activity by in vitro study with DPPH and FRAP methods, determined the content of total phenols, total flavonoids, and also identification of chemical content. Nephrotoxicity study done by induced gentamycin. The groups divided 6 group, consist: negative control, positive control, normal control, and the extract with dose 50 mg/kg, 100 mg/kg, and 200 mg/ kg. The parameter for nephroprotective activity was tubular necrosis, the presence of tubules casts and glomerular damage, creatinine serum, and urea. Results: The ethanol extract has IC 50 162.34 µg/ml for DPPH scavenging activity and 18.65 ± 3.53 FeEAC (Mol/g) for FRAP. The secondary metabolite presence flavonoids, tannins, terpenes, alkaloids, and glycosides. The total phenols 462.36 ± 9.23 mg GAE/gr, total flavonoids content 59.44 ± 0.11 mg QE/gr extract. All the dose have an nephroprotective activity, but the best dose was 50 mg/kg. Conclusion: The ethanol extract of Sterculia rubiginosa showed antioxidant activity and nephroprotective activity.

Phytochemical Analysis and Cytotoxic Activities of Hantap Leaves (<i>Sterculia coccinea</i> Jack) Extract

“…The determination of the levels of secondary metabolites in the leaf extract showed that the most numerous groups of phenolic compounds were tannins and flavonoids, with values of 72.16 and 28.66% w/w. Previous studies showed that phenolics and flavonoids were the most important secondary metabolites in the genus Sterculia [32].…”

The Acute Toxicity of Ki Hampelas Leaves (Sterculia rubiginosa Zoll. Ex Miq)

“…Starting from screening to look for active compounds, then continued with testing the effectiveness or selectivity and its mechanism of action in experimental animals or microbes, after being declared to have certain pharmacological activities by a series of safety tests on experimental animals, the toxicity test 17 . In previous studies, Ki Hampelas leaves have activity as a nephroprotective and antioxidant with a minimum dose of 50 mg/kg 16 . So it is necessary to do a toxicity test to ensure the safety of Ki Hampelas leaves extract.…”

“…The antioxidant activity and total flavonoids equivalent to quercetine 17 . So it is interesting to study whether Sterculia rubiginosa has antioxidant and nephroprotective activity 16 .…”