The neuropeptidomics of Ixodes scapularis synganglion

Ancestral reconstruction of tick lineages

“…A complex network of peptidergic neurons has been identified in the tick synganglion with at least 34 neuropeptides in the I. scapularis genome using bioinformatics, of which 20 neuropeptides could be confirmed using mass spectrometry and localized using antibodies (Christie, 2008;Neupert et al 2009;Šimo et al 2014). More recently, using transcriptome sequencing of the synganglion, 15 neuropeptides and 14 neuropeptide receptors were detected in I. scapularis, while genome screening detected 51 neuropeptides (Egekwu et al 2016;, 16…”

Crustacean neuroendocrine systems and their signaling agents

“…As in crustaceans, mass spectrometry and transcriptome mining have been major contributors to peptide discovery in members of other arthropod subphyla (e.g., Huybrechts et al 2005;Audsley and Weaver 2006;Clynen et al 2006;Christie 2008a, b;Weaver and Audsley 2008;Neupert et al 2009;Ons et al 2009;Christie et al 2011c). However, the sequencing of several insect genomes has provided an additional resource for peptide discovery in members of this taxon (e.g., Hummon et al 2006;Liu et al 2006;Amare and Sweedler 2007;Li et al 2007;Roller et al 2008;Wegener and Gorbashov 2008;Hauser et al 2010;Huybrechts et al 2010;Predel et al 2010).…”

In silico cloning of genes encoding neuropeptides, neurohormones and their putative G-protein coupled receptors in a spider mite

“…Those studies have identified neuropeptide and neuropeptide receptor EST and cDNA sequences and have also identified some peptides directly by mass spectrometry (e.g. Holmes et al, 2003;Simo et al, 2009a,b;Neupert et al, 2009;Donohue et al, 2010;Bissinger et al, 2011). There are also a few publications on the horse shoe crab (e.g.…”